Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth
Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad...
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| Veröffentlicht in: | Journal of integrative plant biology 2011, Vol.53 (1), p.14-24 |
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| creator | Gong, Chunyan Li, Tang Li, Qi Yan, Longfeng Wang, Tai |
| description | Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. Here, we report on the cloning and characterization of OsRAD21-2 from rice (Oryza sativa L.). Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily. Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers. Further RNA in situ hybridization analysis and promoter::β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen, stem intercalary meristem, leaf meristem, and root pericycle. Ectopic expression of OsRAD21-2 in fission yeast resulted in cell growth delay and morphological abnormality. Flow cytometric analysis revealed that the OsRAD21-2-expressed cells were arrested in G2 phase. Our results suggest that OsRad21-2 functions in regulation of cell division and growth. |
| doi_str_mv | 10.1111/j.1744-7909.2010.01009.x |
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In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. Here, we report on the cloning and characterization of OsRAD21-2 from rice (Oryza sativa L.). Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily. Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers. Further RNA in situ hybridization analysis and promoter::β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen, stem intercalary meristem, leaf meristem, and root pericycle. Ectopic expression of OsRAD21-2 in fission yeast resulted in cell growth delay and morphological abnormality. Flow cytometric analysis revealed that the OsRAD21-2-expressed cells were arrested in G2 phase. Our results suggest that OsRad21-2 functions in regulation of cell division and growth.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/j.1744-7909.2010.01009.x</identifier><identifier>PMID: 21205177</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Cell Division ; Chromosomal Proteins, Non-Histone - genetics ; Chromosomal Proteins, Non-Histone - metabolism ; Chromosome Segregation ; Cloning, Molecular ; Oryza - genetics ; Oryza - metabolism ; Oryza sativa ; Phylogeny ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Schizosaccharomyces</subject><ispartof>Journal of integrative plant biology, 2011, Vol.53 (1), p.14-24</ispartof><rights>2011 Institute of Botany, Chinese Academy of Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5609-a9ade5948bde0e310c2720c83e67cd6e23bd41d4dabc3505e0f5bc260e488bac3</citedby><cites>FETCH-LOGICAL-c5609-a9ade5948bde0e310c2720c83e67cd6e23bd41d4dabc3505e0f5bc260e488bac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zwxb/zwxb.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-7909.2010.01009.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-7909.2010.01009.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21205177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gong, Chunyan</creatorcontrib><creatorcontrib>Li, Tang</creatorcontrib><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Yan, Longfeng</creatorcontrib><creatorcontrib>Wang, Tai</creatorcontrib><title>Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth</title><title>Journal of integrative plant biology</title><addtitle>J Integr Plant Biol</addtitle><description>Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. Here, we report on the cloning and characterization of OsRAD21-2 from rice (Oryza sativa L.). Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily. Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers. Further RNA in situ hybridization analysis and promoter::β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen, stem intercalary meristem, leaf meristem, and root pericycle. Ectopic expression of OsRAD21-2 in fission yeast resulted in cell growth delay and morphological abnormality. Flow cytometric analysis revealed that the OsRAD21-2-expressed cells were arrested in G2 phase. Our results suggest that OsRad21-2 functions in regulation of cell division and growth.</description><subject>Cell Division</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>Chromosome Segregation</subject><subject>Cloning, Molecular</subject><subject>Oryza - genetics</subject><subject>Oryza - metabolism</subject><subject>Oryza sativa</subject><subject>Phylogeny</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Schizosaccharomyces</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd9u0zAUhyMEYmPwCuAbBDcp_pPE8Q1S6bYytDHoNiauLMc5Le7SpLPTteUNeGtO2q6XCEuWj-LvfI79iyLCaI_h-DDtMZkksVRU9TjFrzixXD2JDvcbT7HOJI8VlfwgehHClFKR04w_jw444zRlUh5Gf0bOArkMo_4xZzEnLpCT1dxDCFASV5O-bd0DVGty7B5c6eoJuXYhLCAQUyPQIm7bZu7sY5tr6q7vJ5jQkhGERYVMJyrAe1O3ZABVtbFt0M4y9M2y_fUyejY2VYBXu_Uoujk9uR58js8vh2eD_nls04yq2ChTQqqSvCiBgmDUcsmpzQVk0pYZcFGUCSuT0hRWpDQFOk4LyzMKSZ4Xxoqj6O3WuzT12NQTPW0WvsYT9e_lqsDXZN1jCuTebbm5b-7xwq2euWDx500NzSLoPMlypbjMkXz_T5IlCtGMsgTRfIta34TgYazn3s2MX2tGdRetnuouQd0lqLto9SZavcLW17tTFsUMyn3jY5YIfNxdzFWw_m-x_nL27VNXoiDeClxoYbUXGH-nMylkqm-_DvX3i9srJeQPfYH8my0_No02E--CvrlCtaBMCabSVPwFif_KQA</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Gong, Chunyan</creator><creator>Li, Tang</creator><creator>Li, Qi</creator><creator>Yan, Longfeng</creator><creator>Wang, Tai</creator><general>Blackwell Publishing Asia</general><general>Graduate School Graduate School of the Chinese Academy of Sciences,Beijing 100049,China%Research Center for Molecular & Developmental Biology,Key Laboratory of Photosynthesis & Environmental Molecular Physiology,Institute of Botany,the Chinese Academy of Sciences,and National Center for Plant Gene Research,Beijing 100093,China</general><general>Research Center for Molecular & Developmental Biology,Key Laboratory of Photosynthesis & Environmental Molecular Physiology,Institute of Botany,the Chinese Academy of Sciences,and National Center for Plant Gene Research,Beijing 100093,China</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2011</creationdate><title>Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth</title><author>Gong, Chunyan ; Li, Tang ; Li, Qi ; Yan, Longfeng ; Wang, Tai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5609-a9ade5948bde0e310c2720c83e67cd6e23bd41d4dabc3505e0f5bc260e488bac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Cell Division</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>Chromosome Segregation</topic><topic>Cloning, Molecular</topic><topic>Oryza - genetics</topic><topic>Oryza - metabolism</topic><topic>Oryza sativa</topic><topic>Phylogeny</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Schizosaccharomyces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Chunyan</creatorcontrib><creatorcontrib>Li, Tang</creatorcontrib><creatorcontrib>Li, Qi</creatorcontrib><creatorcontrib>Yan, Longfeng</creatorcontrib><creatorcontrib>Wang, Tai</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of integrative plant biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Chunyan</au><au>Li, Tang</au><au>Li, Qi</au><au>Yan, Longfeng</au><au>Wang, Tai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth</atitle><jtitle>Journal of integrative plant biology</jtitle><addtitle>J Integr Plant Biol</addtitle><date>2011</date><risdate>2011</risdate><volume>53</volume><issue>1</issue><spage>14</spage><epage>24</epage><pages>14-24</pages><issn>1672-9072</issn><eissn>1744-7909</eissn><abstract>Rad21 and its meiotic counterpart Rec8, the key components of the cohesin complex, are essential for sister chromatid cohesion and chromosome segregation in mitosis and meiosis, respectively. In contrast to yeast and vertebrates, which have only two RAD21/REC8 genes, the rice genome encodes four Rad21/Rec8 proteins. Here, we report on the cloning and characterization of OsRAD21-2 from rice (Oryza sativa L.). Phylogenetic analysis of the full-length amino acids showed that OsRad21-2 was grouped into the plant-specific Rad21 subfamily. Semi-quantitative reverse transcription-polymerase chain reaction revealed OsRAD21-2 preferentially expressed in premeiotic flowers. Further RNA in situ hybridization analysis and promoter::β-glucuronidase staining indicated that OsRAD21-2 was mainly expressed in actively dividing tissues including premeiotic stamen, stem intercalary meristem, leaf meristem, and root pericycle. Ectopic expression of OsRAD21-2 in fission yeast resulted in cell growth delay and morphological abnormality. Flow cytometric analysis revealed that the OsRAD21-2-expressed cells were arrested in G2 phase. 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| subjects | Cell Division Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosome Segregation Cloning, Molecular Oryza - genetics Oryza - metabolism Oryza sativa Phylogeny Plant Proteins - genetics Plant Proteins - metabolism Schizosaccharomyces |
| title | Rice OsRAD21-2 is Expressed in Actively Dividing Tissues and its Ectopic Expression in Yeast Results in Aberrant Cell Division and Growth |
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