Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava
Cassava （Manihot esculenta Crantz） sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibi...
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| Veröffentlicht in: | Journal of integrative plant biology 2010-07, Vol.52 (7), p.653-669 |
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| creator | Zhang, Peng Wang, Wen-Quan Zhang, Gen-Liang Kaminek, Miroslav Dobrev, Petre Xu, Jia Gruissem, Wilhelm |
| description | Cassava （Manihot esculenta Crantz） sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves. |
| doi_str_mv | 10.1111/j.1744-7909.2010.00956.x |
| format | Article |
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With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves.</description><identifier>ISSN: 1672-9072</identifier><identifier>EISSN: 1744-7909</identifier><identifier>DOI: 10.1111/j.1744-7909.2010.00956.x</identifier><identifier>PMID: 20590995</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>Alkyl and Aryl Transferases - genetics ; Alkyl and Aryl Transferases - metabolism ; Cellular Senescence - genetics ; Cellular Senescence - physiology ; Chlorophyll - metabolism ; Cytokinins - metabolism ; Dehydration ; Droughts ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Plant - physiology ; Manihot - enzymology ; Manihot - genetics ; Plant Leaves - enzymology ; Plant Leaves - genetics ; Plants, Genetically Modified - enzymology ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; 代谢水平 ; 叶片寿命 ; 异戊烯 ; 木薯叶 ; 植物细胞分裂素 ; 衰老抑制 ; 诱导表达 ; 转移酶</subject><ispartof>Journal of integrative plant biology, 2010-07, Vol.52 (7), p.653-669</ispartof><rights>2010 Institute of Botany, Chinese Academy of Sciences</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5796-d8ee3488ca42baa10ce9715f809e1d72b2cc6a32a65b1087a25453319d9ba7d3</citedby><cites>FETCH-LOGICAL-c5796-d8ee3488ca42baa10ce9715f809e1d72b2cc6a32a65b1087a25453319d9ba7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/94176A/94176A.jpg</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1744-7909.2010.00956.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1744-7909.2010.00956.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20590995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Peng</creatorcontrib><creatorcontrib>Wang, Wen-Quan</creatorcontrib><creatorcontrib>Zhang, Gen-Liang</creatorcontrib><creatorcontrib>Kaminek, Miroslav</creatorcontrib><creatorcontrib>Dobrev, Petre</creatorcontrib><creatorcontrib>Xu, Jia</creatorcontrib><creatorcontrib>Gruissem, Wilhelm</creatorcontrib><title>Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava</title><title>Journal of integrative plant biology</title><addtitle>Journal of Integrative Plant Biology</addtitle><description>Cassava （Manihot esculenta Crantz） sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves.</description><subject>Alkyl and Aryl Transferases - genetics</subject><subject>Alkyl and Aryl Transferases - metabolism</subject><subject>Cellular Senescence - genetics</subject><subject>Cellular Senescence - physiology</subject><subject>Chlorophyll - metabolism</subject><subject>Cytokinins - metabolism</subject><subject>Dehydration</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Manihot - enzymology</subject><subject>Manihot - genetics</subject><subject>Plant Leaves - enzymology</subject><subject>Plant Leaves - genetics</subject><subject>Plants, Genetically Modified - enzymology</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>代谢水平</subject><subject>叶片寿命</subject><subject>异戊烯</subject><subject>木薯叶</subject><subject>植物细胞分裂素</subject><subject>衰老抑制</subject><subject>诱导表达</subject><subject>转移酶</subject><issn>1672-9072</issn><issn>1744-7909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcuO0zAUjRCIGQZ-AVlIiA0pjh3HyYLFtPOgqDyn0iytG-em4zZ1OnbCtPMtfCwOLV3jje17z7nHPieKSEJHSVgflqNEpmksC1qMGA1VSguRjbZPotNj42k4Z5LFBZXsJHrh_ZJSntOMPY9OGBUBUYjT6PcNWvQarcZ4aqtem7JBcrndOPTetJa0NZn6doO2Q7tryNyB9TU68AMq1CpPZgg1mZka35Op1Q5Dz5ML1_aLu47cdMMk8hO98R0EGQK2IudNh86Tya5rV8YaS75gB2XbGL8m4TYB7-EXvIye1dB4fHXYz6L51eV88imefbueTs5nsRayyOIqR-RpnmtIWQmQUI2FTESd0wKTSrKSaZ0BZ5CJMqG5BCZSwXlSVEUJsuJn0dv92AewNdiFWra9s0FQPT5sy8FfKikVAfduj9u49r5H36m1CdY1DVhse68k5yI4ztKAzPdI7VrvHdZq48wa3E4lVA0JqqUaglJDUGpQUH8TVNtAfX0Q6cs1Vkfiv8gC4OPhtabB3X8PVp-n38fhFPjxnh_ywO2RD26lMsmlULdfr9XFePzjNivGKg_4N4e_3LV2cW-CPyXoVR3UFU8Fk3km-B8Jl8am</recordid><startdate>201007</startdate><enddate>201007</enddate><creator>Zhang, Peng</creator><creator>Wang, Wen-Quan</creator><creator>Zhang, Gen-Liang</creator><creator>Kaminek, Miroslav</creator><creator>Dobrev, Petre</creator><creator>Xu, Jia</creator><creator>Gruissem, Wilhelm</creator><general>Blackwell Publishing Asia</general><general>Department of Biology, Plant Biotechnology, ETH Zurich, Universit(a)tstrasse 2, 8092 Zurich, Switzerland%Institute of Tropical Biosciences and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China%Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 263, CZ-165 02 Prague 6, Czech Republic%Key Laboratory of Synthetic Biology, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China</general><general>Shanghai Center for Cassava Biotechnology, National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200032, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>201007</creationdate><title>Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava</title><author>Zhang, Peng ; 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With the production of SAG12-1PT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>20590995</pmid><doi>10.1111/j.1744-7909.2010.00956.x</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of integrative plant biology, 2010-07, Vol.52 (7), p.653-669 |
| issn | 1672-9072 1744-7909 |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Alma/SFX Local Collection |
| subjects | Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Cellular Senescence - genetics Cellular Senescence - physiology Chlorophyll - metabolism Cytokinins - metabolism Dehydration Droughts Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology Manihot - enzymology Manihot - genetics Plant Leaves - enzymology Plant Leaves - genetics Plants, Genetically Modified - enzymology Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism 代谢水平 叶片寿命 异戊烯 木薯叶 植物细胞分裂素 衰老抑制 诱导表达 转移酶 |
| title | Senescence-Inducible Expression of Isopentenyl Transferase Extends Leaf Life, Increases Drought Stress Resistance and Alters Cytokinin Metabolism in Cassava |
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