Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis
Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Re...
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| Veröffentlicht in: | Progress in natural science 2002-09, Vol.12 (9), p.713-716 |
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| creator | JIN Jian ZHOU Yan HU Xiaoyan SHI Ning HAN Chunyu XU Caimin QIANG Boqin YUAN Jiangang PENG Xiaozhong |
| description | Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin. |
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Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin.</description><identifier>ISSN: 1002-0071</identifier><language>eng</language><publisher>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, National Human Genome Center, Beijing 100005, China</publisher><subject>apoptosis ; assay ; cerebrum ; fetal ; gene ; hTRXL ; human ; reduction ; thioredoxin-like</subject><ispartof>Progress in natural science, 2002-09, Vol.12 (9), p.713-716</ispartof><rights>Copyright © Wanfang Data Co. Ltd. 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Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin.</description><subject>apoptosis</subject><subject>assay</subject><subject>cerebrum</subject><subject>fetal</subject><subject>gene</subject><subject>hTRXL</subject><subject>human</subject><subject>reduction</subject><subject>thioredoxin-like</subject><issn>1002-0071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNotkD1PwzAQhj2ARCn8Bw8sDJHsWEmcEVV8SZWQKmCNLva5cRLsEDtQ-usxKtNzw_O-p7szsuKM5RljFb8glyH07G8sqxV536FeVISAFFS0Xzb-UG9ot3yAo7GzfkbtD9Zlox2QTrOPaB0Fp6mNgaIxqBK9owrHkcLkp-iDDVfk3MAY8Pqfa_L2cP-6ecq2L4_Pm7ttpnIuY4ZaC6MKAFmVvIICciY5xxalqFutQfC6rIxktRYtE1rXKYaqMBJbXcpcizW5PfV-gzPg9k3vl9mljc1xHg79scE8Hc5qxkVyb06u6rzbf9pkt6AGY0ds0nu4kEVeMPELOONcDw</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>JIN Jian ZHOU Yan HU Xiaoyan SHI Ning HAN Chunyu XU Caimin QIANG Boqin YUAN Jiangang PENG Xiaozhong</creator><general>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, National Human Genome Center, Beijing 100005, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20020901</creationdate><title>Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis</title><author>JIN Jian ZHOU Yan HU Xiaoyan SHI Ning HAN Chunyu XU Caimin QIANG Boqin YUAN Jiangang PENG Xiaozhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c218t-edd3fc5aa87617a5a20811ebe839bdda31967f809d3b03dd9c21ec5f8ebd682d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>apoptosis</topic><topic>assay</topic><topic>cerebrum</topic><topic>fetal</topic><topic>gene</topic><topic>hTRXL</topic><topic>human</topic><topic>reduction</topic><topic>thioredoxin-like</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JIN Jian ZHOU Yan HU Xiaoyan SHI Ning HAN Chunyu XU Caimin QIANG Boqin YUAN Jiangang PENG Xiaozhong</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Progress in natural science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIN Jian ZHOU Yan HU Xiaoyan SHI Ning HAN Chunyu XU Caimin QIANG Boqin YUAN Jiangang PENG Xiaozhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis</atitle><jtitle>Progress in natural science</jtitle><addtitle>Progress in Natural Science</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>12</volume><issue>9</issue><spage>713</spage><epage>716</epage><pages>713-716</pages><issn>1002-0071</issn><abstract>Thioredoxin is a ubiquitous dithiol oxidoreductase found in many organisms and involved in numerous biochemical processes. Human thioredoxin-like protein (hTRXL) is differentially expressed at different development stages of human fetal cerebrum and belongs to an expanding family of thioredoxins. Recombinant hTRXL and truncated hTRXLs corresponding to the N-terminal (hTRXL-N) and C-terminal (hTRXL-C) domains are expressed and purified. In insulin disulfide reduction assay, both full-length hTRXL and hTRXL-N show reducing activity for the insulin disulfide bonds. As expected, the hTRXL-C failed to reduce insulin. MCF-7 cell stably transfected with hTRXL cDNA exhibits increased sensitivity to apoptosis induced by phorbol myristic acetate (PMA) and ionomysin.</abstract><pub>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Peking Union Medical College & Chinese Academy of Medical Sciences, National Human Genome Center, Beijing 100005, China</pub><tpages>4</tpages></addata></record> |
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| ispartof | Progress in natural science, 2002-09, Vol.12 (9), p.713-716 |
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| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | apoptosis assay cerebrum fetal gene hTRXL human reduction thioredoxin-like |
| title | Reductase activity of human thioredoxin-like protein and its effects on cell apoptosis |
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