Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells
To investigate the effects of porous poly lactide-co-glycolide (PLGA) modified by type I collagen on the adhesion, proliferation, and differentiation of rabbit marrow-derived mesenchymal stem cells (MSCs). The third generation MSCs isolated from mature rabbits by density gradient centrifugation were...
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| Veröffentlicht in: | Chinese journal of traumatology 2004-12, Vol.7 (6), p.358-362 |
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| creator | Liu, Gang Hu, Yun-Yu Zhao, Jian-Ning Wu, Su-Jia Xiong, Zhuo Lu, Rong |
| description | To investigate the effects of porous poly lactide-co-glycolide (PLGA) modified by type I collagen on the adhesion, proliferation, and differentiation of rabbit marrow-derived mesenchymal stem cells (MSCs).
The third generation MSCs isolated from mature rabbits by density gradient centrifugation were cultured at different initial concentrations on 0.3 cm x 1.2 cm x 2.0 cm 3-D porous PLGA coated by type I collagen in RPMI 1640 containing 10% fetal calf serum, while cultured on PLGA without type I collagen as control. The cells adhesive and proliferative behavior at 7, 14, and 21 days after inoculation was assessed by determining the incorporation rate of [(3)H]-TdR. In order to examine MSCs differentiation, the expression of osteoblasts marker genes, osteocalcin (OCN), alkaline phosphatase (ALP), osteopontin (OPN) mRNA, were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and further more, the cell morphology at 21 days was also observed by scanning electron microscope (SEM).
Type I collagen promoted cell adhesion on PLGA. The valve was significantly higher than controls (6 h, 2144 cpm+/-141 cpm vs. 1797 cpm+/-118 cpm, P=0.017; 8 h, 2311 cpm+/-113 cpm vs. 1891 cpm+/-103 cpm, P=0.01). The cells which cultured on PLGA coated with type I collagen showed significantly higher cell proliferation than controls on the 7 th day (1021 cpm+/-159 cpm vs. 451 cpm+/-67 cpm, P=0.002), the 14th day (1472 cpm+/-82 cpm vs. 583 cpm+/-67 cpm, P |
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The third generation MSCs isolated from mature rabbits by density gradient centrifugation were cultured at different initial concentrations on 0.3 cm x 1.2 cm x 2.0 cm 3-D porous PLGA coated by type I collagen in RPMI 1640 containing 10% fetal calf serum, while cultured on PLGA without type I collagen as control. The cells adhesive and proliferative behavior at 7, 14, and 21 days after inoculation was assessed by determining the incorporation rate of [(3)H]-TdR. In order to examine MSCs differentiation, the expression of osteoblasts marker genes, osteocalcin (OCN), alkaline phosphatase (ALP), osteopontin (OPN) mRNA, were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and further more, the cell morphology at 21 days was also observed by scanning electron microscope (SEM).
Type I collagen promoted cell adhesion on PLGA. The valve was significantly higher than controls (6 h, 2144 cpm+/-141 cpm vs. 1797 cpm+/-118 cpm, P=0.017; 8 h, 2311 cpm+/-113 cpm vs. 1891 cpm+/-103 cpm, P=0.01). The cells which cultured on PLGA coated with type I collagen showed significantly higher cell proliferation than controls on the 7 th day (1021 cpm+/-159 cpm vs. 451 cpm+/-67 cpm, P=0.002), the 14th day (1472 cpm+/-82 cpm vs. 583 cpm+/-67 cpm, P<0.001) and 21 th day (1728 cpm+/-78 cpm vs. 632 cpm+/-55 cpm, P<0.001). Osteoblasts markers, OCN, ALP, OPN mRNA, were all detected on PLGA coated by type I collagen on the 21 th day, but OCN, OPN mRNA could not be found in controls. Spindle and polygonal cells well distributed on the polymer coated by type I collagen while cylindric or round cells in controls.
Type I collagen is effective in promoting the adhesion, proliferation and differentiation of MSCs on PLGA.</description><identifier>ISSN: 1008-1275</identifier><identifier>PMID: 15566693</identifier><language>eng</language><publisher>China: Department of Orthopedics, Nanjing General Hospital, Nanjing Military District, Nanjing, Jiangsu,210001, China%Institute of Orthopedics of Xijing Hospital, Fourth Military Medicical University, Xi'an, Shanxi, China%Department of Mechanical Engineering of Tsinghua University, Beijing, China</publisher><subject>Biocompatible Materials - pharmacology ; Cell Adhesion ; Cell Proliferation ; Collagen Type I - pharmacology ; Gene Expression ; Humans ; Lactic Acid - pharmacology ; Mesenchymal Stromal Cells - physiology ; Osteoblasts - physiology ; Polyglycolic Acid - pharmacology ; Polymers - pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; Tissue Engineering</subject><ispartof>Chinese journal of traumatology, 2004-12, Vol.7 (6), p.358-362</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zhcszz-e/zhcszz-e.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15566693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Hu, Yun-Yu</creatorcontrib><creatorcontrib>Zhao, Jian-Ning</creatorcontrib><creatorcontrib>Wu, Su-Jia</creatorcontrib><creatorcontrib>Xiong, Zhuo</creatorcontrib><creatorcontrib>Lu, Rong</creatorcontrib><title>Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells</title><title>Chinese journal of traumatology</title><addtitle>Chin J Traumatol</addtitle><description>To investigate the effects of porous poly lactide-co-glycolide (PLGA) modified by type I collagen on the adhesion, proliferation, and differentiation of rabbit marrow-derived mesenchymal stem cells (MSCs).
The third generation MSCs isolated from mature rabbits by density gradient centrifugation were cultured at different initial concentrations on 0.3 cm x 1.2 cm x 2.0 cm 3-D porous PLGA coated by type I collagen in RPMI 1640 containing 10% fetal calf serum, while cultured on PLGA without type I collagen as control. The cells adhesive and proliferative behavior at 7, 14, and 21 days after inoculation was assessed by determining the incorporation rate of [(3)H]-TdR. In order to examine MSCs differentiation, the expression of osteoblasts marker genes, osteocalcin (OCN), alkaline phosphatase (ALP), osteopontin (OPN) mRNA, were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and further more, the cell morphology at 21 days was also observed by scanning electron microscope (SEM).
Type I collagen promoted cell adhesion on PLGA. The valve was significantly higher than controls (6 h, 2144 cpm+/-141 cpm vs. 1797 cpm+/-118 cpm, P=0.017; 8 h, 2311 cpm+/-113 cpm vs. 1891 cpm+/-103 cpm, P=0.01). The cells which cultured on PLGA coated with type I collagen showed significantly higher cell proliferation than controls on the 7 th day (1021 cpm+/-159 cpm vs. 451 cpm+/-67 cpm, P=0.002), the 14th day (1472 cpm+/-82 cpm vs. 583 cpm+/-67 cpm, P<0.001) and 21 th day (1728 cpm+/-78 cpm vs. 632 cpm+/-55 cpm, P<0.001). Osteoblasts markers, OCN, ALP, OPN mRNA, were all detected on PLGA coated by type I collagen on the 21 th day, but OCN, OPN mRNA could not be found in controls. Spindle and polygonal cells well distributed on the polymer coated by type I collagen while cylindric or round cells in controls.
Type I collagen is effective in promoting the adhesion, proliferation and differentiation of MSCs on PLGA.</description><subject>Biocompatible Materials - pharmacology</subject><subject>Cell Adhesion</subject><subject>Cell Proliferation</subject><subject>Collagen Type I - pharmacology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Lactic Acid - pharmacology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Osteoblasts - physiology</subject><subject>Polyglycolic Acid - pharmacology</subject><subject>Polymers - pharmacology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tissue Engineering</subject><issn>1008-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1OwzAQhbMA0VK4AvIKCYlI_knsdImqApUqsYF15NjjJlViBzuhtCfg2Li0rEYz-t7MvHeRTAnGRUqoyCfJdQhbjDOKc3GVTEiec87nbJr8LI0BNSBn0LDvAa2Qcm0rN2CRs2ioAUldQ2icfUS9d21jwMvhr5VWIxcGcFUrw9AoFEWA4Lv3EI6C487KxVEnvXe7VINvvkCjDgJYVe872aIo75CCtg03yaWRbYDbc50lH8_L98Vrun57WS2e1mlPaSFSUck5VkbknGUFnxvDJMuZlBXWhBpCieIFozkIQVSmuckg05pDrpUmGVQVmyUPp707aY20m3LrRm_jxfJQq3A4lEBjTphjLCJ7f2Kj888RwlB2TTh-Ky24MZRcEJyxgkbw7gyOVQe67H0TTe_L_5zZL6H1evY</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Liu, Gang</creator><creator>Hu, Yun-Yu</creator><creator>Zhao, Jian-Ning</creator><creator>Wu, Su-Jia</creator><creator>Xiong, Zhuo</creator><creator>Lu, Rong</creator><general>Department of Orthopedics, Nanjing General Hospital, Nanjing Military District, Nanjing, Jiangsu,210001, China%Institute of Orthopedics of Xijing Hospital, Fourth Military Medicical University, Xi'an, Shanxi, China%Department of Mechanical Engineering of Tsinghua University, Beijing, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20041201</creationdate><title>Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells</title><author>Liu, Gang ; Hu, Yun-Yu ; Zhao, Jian-Ning ; Wu, Su-Jia ; Xiong, Zhuo ; Lu, Rong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2287-7ba90cf75634869ff3a353aab0d12f121c68325e771c4d6f4e4dd6e5dcd14ebb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biocompatible Materials - pharmacology</topic><topic>Cell Adhesion</topic><topic>Cell Proliferation</topic><topic>Collagen Type I - pharmacology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Lactic Acid - pharmacology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Osteoblasts - physiology</topic><topic>Polyglycolic Acid - pharmacology</topic><topic>Polymers - pharmacology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Tissue Engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Gang</creatorcontrib><creatorcontrib>Hu, Yun-Yu</creatorcontrib><creatorcontrib>Zhao, Jian-Ning</creatorcontrib><creatorcontrib>Wu, Su-Jia</creatorcontrib><creatorcontrib>Xiong, Zhuo</creatorcontrib><creatorcontrib>Lu, Rong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese journal of traumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Gang</au><au>Hu, Yun-Yu</au><au>Zhao, Jian-Ning</au><au>Wu, Su-Jia</au><au>Xiong, Zhuo</au><au>Lu, Rong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells</atitle><jtitle>Chinese journal of traumatology</jtitle><addtitle>Chin J Traumatol</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>7</volume><issue>6</issue><spage>358</spage><epage>362</epage><pages>358-362</pages><issn>1008-1275</issn><abstract>To investigate the effects of porous poly lactide-co-glycolide (PLGA) modified by type I collagen on the adhesion, proliferation, and differentiation of rabbit marrow-derived mesenchymal stem cells (MSCs).
The third generation MSCs isolated from mature rabbits by density gradient centrifugation were cultured at different initial concentrations on 0.3 cm x 1.2 cm x 2.0 cm 3-D porous PLGA coated by type I collagen in RPMI 1640 containing 10% fetal calf serum, while cultured on PLGA without type I collagen as control. The cells adhesive and proliferative behavior at 7, 14, and 21 days after inoculation was assessed by determining the incorporation rate of [(3)H]-TdR. In order to examine MSCs differentiation, the expression of osteoblasts marker genes, osteocalcin (OCN), alkaline phosphatase (ALP), osteopontin (OPN) mRNA, were evaluated by reverse transcription-polymerase chain reaction (RT-PCR), and further more, the cell morphology at 21 days was also observed by scanning electron microscope (SEM).
Type I collagen promoted cell adhesion on PLGA. The valve was significantly higher than controls (6 h, 2144 cpm+/-141 cpm vs. 1797 cpm+/-118 cpm, P=0.017; 8 h, 2311 cpm+/-113 cpm vs. 1891 cpm+/-103 cpm, P=0.01). The cells which cultured on PLGA coated with type I collagen showed significantly higher cell proliferation than controls on the 7 th day (1021 cpm+/-159 cpm vs. 451 cpm+/-67 cpm, P=0.002), the 14th day (1472 cpm+/-82 cpm vs. 583 cpm+/-67 cpm, P<0.001) and 21 th day (1728 cpm+/-78 cpm vs. 632 cpm+/-55 cpm, P<0.001). Osteoblasts markers, OCN, ALP, OPN mRNA, were all detected on PLGA coated by type I collagen on the 21 th day, but OCN, OPN mRNA could not be found in controls. Spindle and polygonal cells well distributed on the polymer coated by type I collagen while cylindric or round cells in controls.
Type I collagen is effective in promoting the adhesion, proliferation and differentiation of MSCs on PLGA.</abstract><cop>China</cop><pub>Department of Orthopedics, Nanjing General Hospital, Nanjing Military District, Nanjing, Jiangsu,210001, China%Institute of Orthopedics of Xijing Hospital, Fourth Military Medicical University, Xi'an, Shanxi, China%Department of Mechanical Engineering of Tsinghua University, Beijing, China</pub><pmid>15566693</pmid><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Biocompatible Materials - pharmacology Cell Adhesion Cell Proliferation Collagen Type I - pharmacology Gene Expression Humans Lactic Acid - pharmacology Mesenchymal Stromal Cells - physiology Osteoblasts - physiology Polyglycolic Acid - pharmacology Polymers - pharmacology Reverse Transcriptase Polymerase Chain Reaction Tissue Engineering |
| title | Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells |
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