A simple and efficient method of DNA extraction from formalin-fixed and paraffin-embedded tissues for screening EGFR gene mutation by gene sequencing
Epidermal growth factor receptor （EGFR） is frequently overexpressed in non-small-cell lung cancer （NSCLC） and plays a key role in tumorigenesis.1 Small molecule tyrosine kinase inhibitors （TKIs）,such as gefitinib,erlotinib,and icotinib,which can inhibit receptor tyrosine kinase activity of EGFR beco...
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| Veröffentlicht in: | Chinese medical journal 2014, Vol.127 (11), p.2191-2193 |
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| container_issue | 11 |
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| container_title | Chinese medical journal |
| container_volume | 127 |
| creator | Zhang, Dan Cao, Zhenqiang Zhang, Guizhen Wu, Mei Zhang, Yucheng Du, Zhenwu Zhang, Xuewen |
| description | Epidermal growth factor receptor （EGFR） is frequently overexpressed in non-small-cell lung cancer （NSCLC） and plays a key role in tumorigenesis.1 Small molecule tyrosine kinase inhibitors （TKIs）,such as gefitinib,erlotinib,and icotinib,which can inhibit receptor tyrosine kinase activity of EGFR become clinically available for the treatment of non-small-cell lung cancer （NSCLC）.2 NSCLC patients with EGFR mutation have experienced a marked response to EGFR-TKIs therapy.3 Detection of mutations of the EGFR gene is critical for predicting the response to therapy with TKIs.4 |
| doi_str_mv | 10.3760/cma.j.issn.0366-6999.20140130 |
| format | Article |
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Cao, Zhenqiang ; Zhang, Guizhen ; Wu, Mei ; Zhang, Yucheng ; Du, Zhenwu ; Zhang, Xuewen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-b1b4b3fc7ce0631f9bfafea34d54bee6f335ecbf1eda0f0bc83939e46b427b563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>DNA</topic><topic>DNA - isolation & purification</topic><topic>EGFR</topic><topic>Formaldehyde</topic><topic>Genes, erbB-1 - genetics</topic><topic>Genetic Techniques</topic><topic>Humans</topic><topic>Mutation</topic><topic>Paraffin</topic><topic>Tissue Fixation</topic><topic>基因测序</topic><topic>基因突变</topic><topic>石蜡包埋组织</topic><topic>福尔马林</topic><topic>表皮生长因子受体</topic><topic>酪氨酸激酶抑制剂</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Dan</creatorcontrib><creatorcontrib>Cao, Zhenqiang</creatorcontrib><creatorcontrib>Zhang, Guizhen</creatorcontrib><creatorcontrib>Wu, Mei</creatorcontrib><creatorcontrib>Zhang, Yucheng</creatorcontrib><creatorcontrib>Du, Zhenwu</creatorcontrib><creatorcontrib>Zhang, Xuewen</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Dan</au><au>Cao, Zhenqiang</au><au>Zhang, Guizhen</au><au>Wu, Mei</au><au>Zhang, Yucheng</au><au>Du, Zhenwu</au><au>Zhang, Xuewen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple and efficient method of DNA extraction from formalin-fixed and paraffin-embedded tissues for screening EGFR gene mutation by gene sequencing</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2014</date><risdate>2014</risdate><volume>127</volume><issue>11</issue><spage>2191</spage><epage>2193</epage><pages>2191-2193</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Epidermal growth factor receptor （EGFR） is frequently overexpressed in non-small-cell lung cancer （NSCLC） and plays a key role in tumorigenesis.1 Small molecule tyrosine kinase inhibitors （TKIs）,such as gefitinib,erlotinib,and icotinib,which can inhibit receptor tyrosine kinase activity of EGFR become clinically available for the treatment of non-small-cell lung cancer （NSCLC）.2 NSCLC patients with EGFR mutation have experienced a marked response to EGFR-TKIs therapy.3 Detection of mutations of the EGFR gene is critical for predicting the response to therapy with TKIs.4</abstract><cop>China</cop><pub>Department of Hepatobiliary-pancreatic Surgery, China-Japan Union Hospital, Jilin University,Changchun, Jilin 130033, China%Department of Electrodiagnosis, Second Hospital, Jilin University,Changchun, Jilin 130041, China%Department of Central Laboratory, China-Japan Union Hospital, Jilin University,Changchun, Jilin 130033, China</pub><pmid>24890176</pmid><doi>10.3760/cma.j.issn.0366-6999.20140130</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chinese medical journal, 2014, Vol.127 (11), p.2191-2193 |
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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | DNA DNA - isolation & purification EGFR Formaldehyde Genes, erbB-1 - genetics Genetic Techniques Humans Mutation Paraffin Tissue Fixation 基因测序 基因突变 石蜡包埋组织 福尔马林 表皮生长因子受体 酪氨酸激酶抑制剂 |
| title | A simple and efficient method of DNA extraction from formalin-fixed and paraffin-embedded tissues for screening EGFR gene mutation by gene sequencing |
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