Effect of impaction on gene-modified cells seeded on granular bone allografts in vitro and in vivo
Background While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone graftin...
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description | Background While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.Methods Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.Results A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro.However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.Conclusions Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability. |
doi_str_mv | 10.3760/cma.j.issn.0366-6999.2010.21.019 |
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A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.Methods Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.Results A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro.However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.Conclusions Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><identifier>DOI: 10.3760/cma.j.issn.0366-6999.2010.21.019</identifier><identifier>PMID: 21162955</identifier><language>eng</language><publisher>China: Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, China%Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China</publisher><subject>Animals ; Bone Marrow Cells - cytology ; Bone Transplantation - methods ; Cell Survival - physiology ; Cells, Cultured ; Dogs ; Male ; Microscopy, Fluorescence ; Stem Cells - cytology ; Stromal Cells - cytology ; Transplantation, Homologous ; 基因修饰 ; 基质细胞 ; 绿色荧光蛋白 ; 骨髓基质干细胞</subject><ispartof>Chinese medical journal, 2010-11, Vol.123 (21), p.3055-3060</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21162955$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Zhen</creatorcontrib><creatorcontrib>Mao, Yuan-Qing</creatorcontrib><creatorcontrib>Zhu, Zhen-An</creatorcontrib><title>Effect of impaction on gene-modified cells seeded on granular bone allografts in vitro and in vivo</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.Methods Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.Results A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro.However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.Conclusions Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability.</description><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Transplantation - methods</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Dogs</subject><subject>Male</subject><subject>Microscopy, Fluorescence</subject><subject>Stem Cells - cytology</subject><subject>Stromal Cells - cytology</subject><subject>Transplantation, Homologous</subject><subject>基因修饰</subject><subject>基质细胞</subject><subject>绿色荧光蛋白</subject><subject>骨髓基质干细胞</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1v1DAQhi0EotvCX0BWD9BLgseJHfuIVqWtVIkLnKOJP7beJvY2Torg1-PVlkojjWbm0bwzLyFXwOqmk-yrmbDe1yHnWLNGykpqrWvOyphDzUC_IRsuWl4J2cJbsnllzsh5znvGuBCdfE_OOIDkWogNGa69d2ahydMwHdAsIUVaYueiq6Zkgw_OUuPGMdPsnC3FcTpjXEec6ZCioziOqXT8kmmI9Dksc6IY7al4Th_IO49jdh9f8gX59f365_a2uv9xc7f9dl8ZLtVSdW3ntVeu0wN02CFHYUBpLx0MzjhogTXCWIDWcl3uV0pw3nhrgaFF6JoL8vm09zdGj3HX79M6x6LY_30w0_5oE4diUgG_nMDDnJ5Wl5d-Cvn4IkaX1twrULJtuVCF_PRCrsPkbH-Yw4Tzn_6_fwW4PAHmIcXdUyiqA5pHH0bXN0JxKVrZ_AO-mIGE</recordid><startdate>20101105</startdate><enddate>20101105</enddate><creator>Yuan, Zhen</creator><creator>Mao, Yuan-Qing</creator><creator>Zhu, Zhen-An</creator><general>Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, China%Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China</general><general>Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20101105</creationdate><title>Effect of impaction on gene-modified cells seeded on granular bone allografts in vitro and in vivo</title><author>Yuan, Zhen ; Mao, Yuan-Qing ; Zhu, Zhen-An</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-747f9f8e79b17a7a2a5c189f6e1bece141035cd114d29116885223fdd10ada173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Transplantation - methods</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Dogs</topic><topic>Male</topic><topic>Microscopy, Fluorescence</topic><topic>Stem Cells - cytology</topic><topic>Stromal Cells - cytology</topic><topic>Transplantation, Homologous</topic><topic>基因修饰</topic><topic>基质细胞</topic><topic>绿色荧光蛋白</topic><topic>骨髓基质干细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Zhen</creatorcontrib><creatorcontrib>Mao, Yuan-Qing</creatorcontrib><creatorcontrib>Zhu, Zhen-An</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Zhen</au><au>Mao, Yuan-Qing</au><au>Zhu, Zhen-An</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of impaction on gene-modified cells seeded on granular bone allografts in vitro and in vivo</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2010-11-05</date><risdate>2010</risdate><volume>123</volume><issue>21</issue><spage>3055</spage><epage>3060</epage><pages>3055-3060</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.Methods Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.Results A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro.However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.Conclusions Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability.</abstract><cop>China</cop><pub>Department of Orthopedics, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, China%Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China</pub><pmid>21162955</pmid><doi>10.3760/cma.j.issn.0366-6999.2010.21.019</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Bone Marrow Cells - cytology Bone Transplantation - methods Cell Survival - physiology Cells, Cultured Dogs Male Microscopy, Fluorescence Stem Cells - cytology Stromal Cells - cytology Transplantation, Homologous 基因修饰 基质细胞 绿色荧光蛋白 骨髓基质干细胞 |
title | Effect of impaction on gene-modified cells seeded on granular bone allografts in vitro and in vivo |
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