Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture
Background Embryonic stem （ES） cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodege...
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| Veröffentlicht in: | Chinese medical journal 2010-03, Vol.123 (6), p.734-738 |
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| creator | Yao, Xing Zhang, Jia-rong Huang, Hua-rong Dai, Li-cheng Liu, Qing-jun Zhang, Ming |
| description | Background Embryonic stem （ES） cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase （HDAC） inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study,we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cellselection.Methods In this study, we used HDAC inhibitor sodium butyrate （NAB） to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system. Results Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable. Conclusion The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation. |
| doi_str_mv | 10.3760/cma.j.issn.0366-6999.2010.06.018 |
| format | Article |
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Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase （HDAC） inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study,we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cellselection.Methods In this study, we used HDAC inhibitor sodium butyrate （NAB） to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system. Results Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable. Conclusion The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><identifier>DOI: 10.3760/cma.j.issn.0366-6999.2010.06.018</identifier><identifier>PMID: 20368096</identifier><language>eng</language><publisher>China: Department of Hepatopancreatobilary Surgery, Huzhon Central Hospital, Huzhou, Zhejiang 313000, China%College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310058, China%Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China</publisher><subject>Animals ; Butyrates - pharmacology ; Cell Adhesion ; Cell Cycle - drug effects ; Cell Differentiation - drug effects ; Cells, Cultured ; Embryonic Stem Cells - cytology ; Embryonic Stem Cells - drug effects ; Fibroblast Growth Factor 2 - pharmacology ; Histone Deacetylase Inhibitors - pharmacology ; Mice ; Neurons - cytology ; Neurons - physiology ; 神经发生 ; 组蛋白乙酰化 ; 组蛋白去乙酰化酶 ; 细胞分化 ; 细胞粘附 ; 胚胎干细胞 ; 诱导分化 ; 酶抑制剂</subject><ispartof>Chinese medical journal, 2010-03, Vol.123 (6), p.734-738</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-efd774309b3ce8b1b99fedaa4eac9780896457f5b276c4601eb61aebde59ebe03</citedby><cites>FETCH-LOGICAL-c446t-efd774309b3ce8b1b99fedaa4eac9780896457f5b276c4601eb61aebde59ebe03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20368096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Xing</creatorcontrib><creatorcontrib>Zhang, Jia-rong</creatorcontrib><creatorcontrib>Huang, Hua-rong</creatorcontrib><creatorcontrib>Dai, Li-cheng</creatorcontrib><creatorcontrib>Liu, Qing-jun</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><title>Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background Embryonic stem （ES） cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase （HDAC） inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study,we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cellselection.Methods In this study, we used HDAC inhibitor sodium butyrate （NAB） to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system. Results Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable. Conclusion The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation.</description><subject>Animals</subject><subject>Butyrates - pharmacology</subject><subject>Cell Adhesion</subject><subject>Cell Cycle - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - drug effects</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Histone Deacetylase Inhibitors - pharmacology</subject><subject>Mice</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>神经发生</subject><subject>组蛋白乙酰化</subject><subject>组蛋白去乙酰化酶</subject><subject>细胞分化</subject><subject>细胞粘附</subject><subject>胚胎干细胞</subject><subject>诱导分化</subject><subject>酶抑制剂</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUGP1SAUhYnROM_Rv2CIC51NKy2Ulp2TiTomk7jRNQF6mUdtYQZoxuevl_qeb0FIuN-5514OQlcNqWnPyUezqHqqXUq-JpTzigsh6paUMuE1aYZnaNd2rK06zprnaHdmLtCrlCZC2q7r-Ut00ZbKQATfod-3LuXgAY-gDOTDrBJg5_dOuxwifohhCRkSHp21EMFnp7ILHgeLYdHxELwzOGVYsIF5TkWaA_awRjWfX7Aa9_-0eAk-mHXOa4TX6IVVc4I3p_sS_fzy-cfNbXX3_eu3m-u7yjDGcwV27HtGidDUwKAbLYSFUSlWxhX9QAbBWdfbTrc9N4yTBjRvFOgROgEaCL1E7499n5S3yt_LKazRF0f5Z2-Wafs8UmRDAT8cwbLz4wopy8WlbQXlIaxJ9pSy7YhCfjqSJoaUIlj5EN2i4kE2RG45yZKTnOSWk9wykFsGcrOShMuj2duT2aoXGM8N_gdTgHcnj33w94-uDK6V-WXdDJKyltOBEfoXX3-hgw</recordid><startdate>20100320</startdate><enddate>20100320</enddate><creator>Yao, Xing</creator><creator>Zhang, Jia-rong</creator><creator>Huang, Hua-rong</creator><creator>Dai, Li-cheng</creator><creator>Liu, Qing-jun</creator><creator>Zhang, Ming</creator><general>Department of Hepatopancreatobilary Surgery, Huzhon Central Hospital, Huzhou, Zhejiang 313000, China%College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310058, China%Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20100320</creationdate><title>Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture</title><author>Yao, Xing ; Zhang, Jia-rong ; Huang, Hua-rong ; Dai, Li-cheng ; Liu, Qing-jun ; Zhang, Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-efd774309b3ce8b1b99fedaa4eac9780896457f5b276c4601eb61aebde59ebe03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Butyrates - pharmacology</topic><topic>Cell Adhesion</topic><topic>Cell Cycle - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Embryonic Stem Cells - drug effects</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Histone Deacetylase Inhibitors - pharmacology</topic><topic>Mice</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>神经发生</topic><topic>组蛋白乙酰化</topic><topic>组蛋白去乙酰化酶</topic><topic>细胞分化</topic><topic>细胞粘附</topic><topic>胚胎干细胞</topic><topic>诱导分化</topic><topic>酶抑制剂</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yao, Xing</creatorcontrib><creatorcontrib>Zhang, Jia-rong</creatorcontrib><creatorcontrib>Huang, Hua-rong</creatorcontrib><creatorcontrib>Dai, Li-cheng</creatorcontrib><creatorcontrib>Liu, Qing-jun</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yao, Xing</au><au>Zhang, Jia-rong</au><au>Huang, Hua-rong</au><au>Dai, Li-cheng</au><au>Liu, Qing-jun</au><au>Zhang, Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2010-03-20</date><risdate>2010</risdate><volume>123</volume><issue>6</issue><spage>734</spage><epage>738</epage><pages>734-738</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background Embryonic stem （ES） cells poss unlimited self-renewal capacity and the ability to differentiate into cell of all three germ layers in vitro. Induced differentiation of ES cells to neural lineage cells has great potential in basic study of neurogenesis and regeneration therapy of neurodegenerative diseases. Histone deacetylase （HDAC） inhibitors enhance histone acetylation so that globularly activate gene expression and may initiate multilineage differentiation. In this study,we aimed to develop a method to induce the differentiation of ES cells to neural cells combining HDAC inhibition and neural cellselection.Methods In this study, we used HDAC inhibitor sodium butyrate （NAB） to induce the differentiation of mouse embryonic stem cells to neural cells through monolayer culture. After differentiation initiation by histone deacetylase inhibitor sodium butyrate, neural cells were induced and selected with a serum free culture system. Results Homogeneous neurons without glial cells demonstrated by molecular marker expression were differentiated with the method. The resultant neurons were excitable. Conclusion The method combined differentiation induction effect of HDAC inhibitors and selective culture system to derive neural cells from ES cells, and implied the involvement of epigenetic regulation in neural differentiation.</abstract><cop>China</cop><pub>Department of Hepatopancreatobilary Surgery, Huzhon Central Hospital, Huzhou, Zhejiang 313000, China%College of Life Science, Zhejiang University, Hangzhou, Zhejiang 310058, China%Biosensor National Special Laboratory, Department of Biomedical Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China</pub><pmid>20368096</pmid><doi>10.3760/cma.j.issn.0366-6999.2010.06.018</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Butyrates - pharmacology Cell Adhesion Cell Cycle - drug effects Cell Differentiation - drug effects Cells, Cultured Embryonic Stem Cells - cytology Embryonic Stem Cells - drug effects Fibroblast Growth Factor 2 - pharmacology Histone Deacetylase Inhibitors - pharmacology Mice Neurons - cytology Neurons - physiology 神经发生 组蛋白乙酰化 组蛋白去乙酰化酶 细胞分化 细胞粘附 胚胎干细胞 诱导分化 酶抑制剂 |
| title | Histone deacetylase inhibitor promotes differentiation of embryonic stem cells into neural cells in adherent monoculture |
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