Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats
Background Cell transplantation for myocardial repair is limited by early cell death. Gene therapy with human growth hormone （hGH） has been shown to promote angiogensis and attenuate apoptosis in the experimental animal. This study was conducted to explore the effects of myoblast-based hGH gene ther...
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| creator | Rong, Shu-ling Lu, Yong-xin Liao, Yu-hua Wang, Xiao-lin Wang, Yong-jin Chang, Chao Wang, Yu-qin Liu, Qi-yun Gao, Yan-zhang Mi, Shao-hua |
| description | Background Cell transplantation for myocardial repair is limited by early cell death. Gene therapy with human growth hormone （hGH） has been shown to promote angiogensis and attenuate apoptosis in the experimental animal. This study was conducted to explore the effects of myoblast-based hGH gene therapy on heart function restoration and angiogenesis after myocardial infarction, and to compare the differences between myoblast-based hGH gene therapy and myoblast therapy.
Methods Myoblasts were isolated from several SD rats, cultured, purified, and transfected with plasmid pLghGHSN and pLgGFPSN. Radioimmunoassay （RIA） was used to detect the expression of hGH in these myoblasts. SD rats underwent the ligation of the left anterior descending coronary artery so as to establish a heart ischemia model. Thirty surviving rats that underwent ligation were randomly divided into 3 equal groups 2 weeks after left coronary artery occlusion： pLghGHSN group received myoblast infected with hGH gene transplantation; pLgGFPSN group received myoblast infected with GFP gene transplantation; control group： received cultured medium only. Four weeks after the injection the surviving rat underwent evaluation of cardiac function by echocardiography. The rats were killed and ventricular samples were undergone immunohistochemistry with hematoxylin-eosin and factor Ⅷ. Cryosection was analyzed by fluorescence microscopy to examine the expression of green fluorescent protein. Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to examine the mRNA expression of vascular endothelial growth factor （VEGF）, bax and Bcl-2. hGH expression in myocardium was examined by Western blot.
Results Myoblast can be successfully isolated, cultured and transfected. The expression of hGH in transfected myoblast was demonstrated with RIA. Four weeks after therapy, the cardiac function was improved significantly in pLghGHSN group and pLgGFPSN group. Fractional shortening （FS） and ejection fraction （EF） in pLghGHSN group were elevated significantly compared with pLgGFPSN group and control group after therapy （FS： 36.9±5.3 vs 29.5±3.5, 21.8±2.9; EF： 56.9±4.3 vs 47.1±3.6, 38.4±4.8, P〈0.05）. Left ventricular end-diastolic dimension （LVEDD） and heart infracted size in pLghGHSN group were decreased significantly compared with pLgGFPSN group and control group after therapy （LVEDD： 5.9±0.3 vs 6.8±0.2, 8.6±0.3; heart infracted size： （34.5±4.2）% vs （40.0±3.9）%, （46.1±3.8）%, P〈0.05）; Green fluorescence was |
| doi_str_mv | 10.1097/00029330-200802020-00013 |
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| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_zhcmj200804013</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>26545576</cqvip_id><wanfj_id>zhcmj200804013</wanfj_id><sourcerecordid>zhcmj200804013</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-2a6e0209486f86f146765c081fa7ac1e9918773f11c411e4efaf803914d28d493</originalsourceid><addsrcrecordid>eNpFUV1vFCEUJUZj1-pfMMQH30b5GgYeTVNbkya-6DNhWdiddYAtMN1U_7x3tqsNhBsO59zLvQchTMknSvTwmRDCNOekY4QowmB1AFH-Aq1YL1jXS0FfohXhUnZSa32B3tS6B1HfD_I1uqCKEyGkXqE_1yF41yrOAbdiUz1MNjW_wfExrydb4eUELyRAj2Pb4d0cbcLbko_LJZeYk8dbD0dOeIyHkh989KktOR8gltHNky04zMm1ETiAF9vqW_Qq2Kn6d-d4iX5-vf5xddvdfb_5dvXlrnOC0dYxKz10qIWSATYVcpC9I4oGO1hHvdZUDQMPlDpBqRc-2KAI11RsmNoIzS_Rx6e8R5uCTVuzz3NJUNH83rm4P81QwPSeidDC_exrM3Gszk8wEp_nagbCheRcAVE9EV3JtRYfzKGM0ZZHQ4lZHDL_HDL_HTInh0D6_lxjXke_eRaeLQHCh3PuXU7b-xH-u7buVxgnb5jsxWIh_wsORZiD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70346338</pqid></control><display><type>article</type><title>Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Rong, Shu-ling ; Lu, Yong-xin ; Liao, Yu-hua ; Wang, Xiao-lin ; Wang, Yong-jin ; Chang, Chao ; Wang, Yu-qin ; Liu, Qi-yun ; Gao, Yan-zhang ; Mi, Shao-hua</creator><creatorcontrib>Rong, Shu-ling ; Lu, Yong-xin ; Liao, Yu-hua ; Wang, Xiao-lin ; Wang, Yong-jin ; Chang, Chao ; Wang, Yu-qin ; Liu, Qi-yun ; Gao, Yan-zhang ; Mi, Shao-hua</creatorcontrib><description>Background Cell transplantation for myocardial repair is limited by early cell death. Gene therapy with human growth hormone （hGH） has been shown to promote angiogensis and attenuate apoptosis in the experimental animal. This study was conducted to explore the effects of myoblast-based hGH gene therapy on heart function restoration and angiogenesis after myocardial infarction, and to compare the differences between myoblast-based hGH gene therapy and myoblast therapy.
Methods Myoblasts were isolated from several SD rats, cultured, purified, and transfected with plasmid pLghGHSN and pLgGFPSN. Radioimmunoassay （RIA） was used to detect the expression of hGH in these myoblasts. SD rats underwent the ligation of the left anterior descending coronary artery so as to establish a heart ischemia model. Thirty surviving rats that underwent ligation were randomly divided into 3 equal groups 2 weeks after left coronary artery occlusion： pLghGHSN group received myoblast infected with hGH gene transplantation; pLgGFPSN group received myoblast infected with GFP gene transplantation; control group： received cultured medium only. Four weeks after the injection the surviving rat underwent evaluation of cardiac function by echocardiography. The rats were killed and ventricular samples were undergone immunohistochemistry with hematoxylin-eosin and factor Ⅷ. Cryosection was analyzed by fluorescence microscopy to examine the expression of green fluorescent protein. Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to examine the mRNA expression of vascular endothelial growth factor （VEGF）, bax and Bcl-2. hGH expression in myocardium was examined by Western blot.
Results Myoblast can be successfully isolated, cultured and transfected. The expression of hGH in transfected myoblast was demonstrated with RIA. Four weeks after therapy, the cardiac function was improved significantly in pLghGHSN group and pLgGFPSN group. Fractional shortening （FS） and ejection fraction （EF） in pLghGHSN group were elevated significantly compared with pLgGFPSN group and control group after therapy （FS： 36.9±5.3 vs 29.5±3.5, 21.8±2.9; EF： 56.9±4.3 vs 47.1±3.6, 38.4±4.8, P〈0.05）. Left ventricular end-diastolic dimension （LVEDD） and heart infracted size in pLghGHSN group were decreased significantly compared with pLgGFPSN group and control group after therapy （LVEDD： 5.9±0.3 vs 6.8±0.2, 8.6±0.3; heart infracted size： （34.5±4.2）% vs （40.0±3.9）%, （46.1±3.8）%, P〈0.05）; Green fluorescence was detected in cryosection of pLgGFPSN group. The capillary density of the pLgGFPSN group was significantly greater than those of the pLghGHSN group and control group （P〈0.05）. The mRNA expression of VEGF and Bcl-2/bax in pLghGHSN group was higher than in pLgGFPSN group or control group （P〈0.05）. The expression of hGH clene in myocardium tissue can be detected by Westem blot assay in pLghGHSN group.
Conclusions Transplantation of heart cells transfected with hGH induced greater angiogenesis and effect of antiapoptosis than transplantation of cells transfected with GFP. Combined GH gene transfer and cell transplantation provided an effective strategy for improving postinfarction ventricular function.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><identifier>DOI: 10.1097/00029330-200802020-00013</identifier><identifier>PMID: 18304469</identifier><language>eng</language><publisher>China: Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430022,China%Department of Cardiology,Heping Hospital,Changzhi Medical College,Changzhi,Shanxi 046000,China</publisher><subject>Animals ; Blotting, Western ; Cells, Cultured ; Echocardiography ; Genetic Therapy ; Human Growth Hormone - blood ; Human Growth Hormone - genetics ; Immunohistochemistry ; Myoblasts, Skeletal - transplantation ; Myocardial Infarction - physiopathology ; Myocardial Infarction - therapy ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - analysis ; Transfection ; Ventricular Function ; 基因治疗 ; 心脏功能 ; 成肌细胞 ; 生长激素</subject><ispartof>Chinese medical journal, 2008-02, Vol.121 (4), p.347-354</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-2a6e0209486f86f146765c081fa7ac1e9918773f11c411e4efaf803914d28d493</citedby><cites>FETCH-LOGICAL-c421t-2a6e0209486f86f146765c081fa7ac1e9918773f11c411e4efaf803914d28d493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18304469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rong, Shu-ling</creatorcontrib><creatorcontrib>Lu, Yong-xin</creatorcontrib><creatorcontrib>Liao, Yu-hua</creatorcontrib><creatorcontrib>Wang, Xiao-lin</creatorcontrib><creatorcontrib>Wang, Yong-jin</creatorcontrib><creatorcontrib>Chang, Chao</creatorcontrib><creatorcontrib>Wang, Yu-qin</creatorcontrib><creatorcontrib>Liu, Qi-yun</creatorcontrib><creatorcontrib>Gao, Yan-zhang</creatorcontrib><creatorcontrib>Mi, Shao-hua</creatorcontrib><title>Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background Cell transplantation for myocardial repair is limited by early cell death. Gene therapy with human growth hormone （hGH） has been shown to promote angiogensis and attenuate apoptosis in the experimental animal. This study was conducted to explore the effects of myoblast-based hGH gene therapy on heart function restoration and angiogenesis after myocardial infarction, and to compare the differences between myoblast-based hGH gene therapy and myoblast therapy.
Methods Myoblasts were isolated from several SD rats, cultured, purified, and transfected with plasmid pLghGHSN and pLgGFPSN. Radioimmunoassay （RIA） was used to detect the expression of hGH in these myoblasts. SD rats underwent the ligation of the left anterior descending coronary artery so as to establish a heart ischemia model. Thirty surviving rats that underwent ligation were randomly divided into 3 equal groups 2 weeks after left coronary artery occlusion： pLghGHSN group received myoblast infected with hGH gene transplantation; pLgGFPSN group received myoblast infected with GFP gene transplantation; control group： received cultured medium only. Four weeks after the injection the surviving rat underwent evaluation of cardiac function by echocardiography. The rats were killed and ventricular samples were undergone immunohistochemistry with hematoxylin-eosin and factor Ⅷ. Cryosection was analyzed by fluorescence microscopy to examine the expression of green fluorescent protein. Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to examine the mRNA expression of vascular endothelial growth factor （VEGF）, bax and Bcl-2. hGH expression in myocardium was examined by Western blot.
Results Myoblast can be successfully isolated, cultured and transfected. The expression of hGH in transfected myoblast was demonstrated with RIA. Four weeks after therapy, the cardiac function was improved significantly in pLghGHSN group and pLgGFPSN group. Fractional shortening （FS） and ejection fraction （EF） in pLghGHSN group were elevated significantly compared with pLgGFPSN group and control group after therapy （FS： 36.9±5.3 vs 29.5±3.5, 21.8±2.9; EF： 56.9±4.3 vs 47.1±3.6, 38.4±4.8, P〈0.05）. Left ventricular end-diastolic dimension （LVEDD） and heart infracted size in pLghGHSN group were decreased significantly compared with pLgGFPSN group and control group after therapy （LVEDD： 5.9±0.3 vs 6.8±0.2, 8.6±0.3; heart infracted size： （34.5±4.2）% vs （40.0±3.9）%, （46.1±3.8）%, P〈0.05）; Green fluorescence was detected in cryosection of pLgGFPSN group. The capillary density of the pLgGFPSN group was significantly greater than those of the pLghGHSN group and control group （P〈0.05）. The mRNA expression of VEGF and Bcl-2/bax in pLghGHSN group was higher than in pLgGFPSN group or control group （P〈0.05）. The expression of hGH clene in myocardium tissue can be detected by Westem blot assay in pLghGHSN group.
Conclusions Transplantation of heart cells transfected with hGH induced greater angiogenesis and effect of antiapoptosis than transplantation of cells transfected with GFP. Combined GH gene transfer and cell transplantation provided an effective strategy for improving postinfarction ventricular function.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cells, Cultured</subject><subject>Echocardiography</subject><subject>Genetic Therapy</subject><subject>Human Growth Hormone - blood</subject><subject>Human Growth Hormone - genetics</subject><subject>Immunohistochemistry</subject><subject>Myoblasts, Skeletal - transplantation</subject><subject>Myocardial Infarction - physiopathology</subject><subject>Myocardial Infarction - therapy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - analysis</subject><subject>Transfection</subject><subject>Ventricular Function</subject><subject>基因治疗</subject><subject>心脏功能</subject><subject>成肌细胞</subject><subject>生长激素</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUV1vFCEUJUZj1-pfMMQH30b5GgYeTVNbkya-6DNhWdiddYAtMN1U_7x3tqsNhBsO59zLvQchTMknSvTwmRDCNOekY4QowmB1AFH-Aq1YL1jXS0FfohXhUnZSa32B3tS6B1HfD_I1uqCKEyGkXqE_1yF41yrOAbdiUz1MNjW_wfExrydb4eUELyRAj2Pb4d0cbcLbko_LJZeYk8dbD0dOeIyHkh989KktOR8gltHNky04zMm1ETiAF9vqW_Qq2Kn6d-d4iX5-vf5xddvdfb_5dvXlrnOC0dYxKz10qIWSATYVcpC9I4oGO1hHvdZUDQMPlDpBqRc-2KAI11RsmNoIzS_Rx6e8R5uCTVuzz3NJUNH83rm4P81QwPSeidDC_exrM3Gszk8wEp_nagbCheRcAVE9EV3JtRYfzKGM0ZZHQ4lZHDL_HDL_HTInh0D6_lxjXke_eRaeLQHCh3PuXU7b-xH-u7buVxgnb5jsxWIh_wsORZiD</recordid><startdate>20080220</startdate><enddate>20080220</enddate><creator>Rong, Shu-ling</creator><creator>Lu, Yong-xin</creator><creator>Liao, Yu-hua</creator><creator>Wang, Xiao-lin</creator><creator>Wang, Yong-jin</creator><creator>Chang, Chao</creator><creator>Wang, Yu-qin</creator><creator>Liu, Qi-yun</creator><creator>Gao, Yan-zhang</creator><creator>Mi, Shao-hua</creator><general>Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430022,China%Department of Cardiology,Heping Hospital,Changzhi Medical College,Changzhi,Shanxi 046000,China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20080220</creationdate><title>Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats</title><author>Rong, Shu-ling ; Lu, Yong-xin ; Liao, Yu-hua ; Wang, Xiao-lin ; Wang, Yong-jin ; Chang, Chao ; Wang, Yu-qin ; Liu, Qi-yun ; Gao, Yan-zhang ; Mi, Shao-hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-2a6e0209486f86f146765c081fa7ac1e9918773f11c411e4efaf803914d28d493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cells, Cultured</topic><topic>Echocardiography</topic><topic>Genetic Therapy</topic><topic>Human Growth Hormone - blood</topic><topic>Human Growth Hormone - genetics</topic><topic>Immunohistochemistry</topic><topic>Myoblasts, Skeletal - transplantation</topic><topic>Myocardial Infarction - physiopathology</topic><topic>Myocardial Infarction - therapy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - analysis</topic><topic>Transfection</topic><topic>Ventricular Function</topic><topic>基因治疗</topic><topic>心脏功能</topic><topic>成肌细胞</topic><topic>生长激素</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rong, Shu-ling</creatorcontrib><creatorcontrib>Lu, Yong-xin</creatorcontrib><creatorcontrib>Liao, Yu-hua</creatorcontrib><creatorcontrib>Wang, Xiao-lin</creatorcontrib><creatorcontrib>Wang, Yong-jin</creatorcontrib><creatorcontrib>Chang, Chao</creatorcontrib><creatorcontrib>Wang, Yu-qin</creatorcontrib><creatorcontrib>Liu, Qi-yun</creatorcontrib><creatorcontrib>Gao, Yan-zhang</creatorcontrib><creatorcontrib>Mi, Shao-hua</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rong, Shu-ling</au><au>Lu, Yong-xin</au><au>Liao, Yu-hua</au><au>Wang, Xiao-lin</au><au>Wang, Yong-jin</au><au>Chang, Chao</au><au>Wang, Yu-qin</au><au>Liu, Qi-yun</au><au>Gao, Yan-zhang</au><au>Mi, Shao-hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2008-02-20</date><risdate>2008</risdate><volume>121</volume><issue>4</issue><spage>347</spage><epage>354</epage><pages>347-354</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background Cell transplantation for myocardial repair is limited by early cell death. Gene therapy with human growth hormone （hGH） has been shown to promote angiogensis and attenuate apoptosis in the experimental animal. This study was conducted to explore the effects of myoblast-based hGH gene therapy on heart function restoration and angiogenesis after myocardial infarction, and to compare the differences between myoblast-based hGH gene therapy and myoblast therapy.
Methods Myoblasts were isolated from several SD rats, cultured, purified, and transfected with plasmid pLghGHSN and pLgGFPSN. Radioimmunoassay （RIA） was used to detect the expression of hGH in these myoblasts. SD rats underwent the ligation of the left anterior descending coronary artery so as to establish a heart ischemia model. Thirty surviving rats that underwent ligation were randomly divided into 3 equal groups 2 weeks after left coronary artery occlusion： pLghGHSN group received myoblast infected with hGH gene transplantation; pLgGFPSN group received myoblast infected with GFP gene transplantation; control group： received cultured medium only. Four weeks after the injection the surviving rat underwent evaluation of cardiac function by echocardiography. The rats were killed and ventricular samples were undergone immunohistochemistry with hematoxylin-eosin and factor Ⅷ. Cryosection was analyzed by fluorescence microscopy to examine the expression of green fluorescent protein. Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to examine the mRNA expression of vascular endothelial growth factor （VEGF）, bax and Bcl-2. hGH expression in myocardium was examined by Western blot.
Results Myoblast can be successfully isolated, cultured and transfected. The expression of hGH in transfected myoblast was demonstrated with RIA. Four weeks after therapy, the cardiac function was improved significantly in pLghGHSN group and pLgGFPSN group. Fractional shortening （FS） and ejection fraction （EF） in pLghGHSN group were elevated significantly compared with pLgGFPSN group and control group after therapy （FS： 36.9±5.3 vs 29.5±3.5, 21.8±2.9; EF： 56.9±4.3 vs 47.1±3.6, 38.4±4.8, P〈0.05）. Left ventricular end-diastolic dimension （LVEDD） and heart infracted size in pLghGHSN group were decreased significantly compared with pLgGFPSN group and control group after therapy （LVEDD： 5.9±0.3 vs 6.8±0.2, 8.6±0.3; heart infracted size： （34.5±4.2）% vs （40.0±3.9）%, （46.1±3.8）%, P〈0.05）; Green fluorescence was detected in cryosection of pLgGFPSN group. The capillary density of the pLgGFPSN group was significantly greater than those of the pLghGHSN group and control group （P〈0.05）. The mRNA expression of VEGF and Bcl-2/bax in pLghGHSN group was higher than in pLgGFPSN group or control group （P〈0.05）. The expression of hGH clene in myocardium tissue can be detected by Westem blot assay in pLghGHSN group.
Conclusions Transplantation of heart cells transfected with hGH induced greater angiogenesis and effect of antiapoptosis than transplantation of cells transfected with GFP. Combined GH gene transfer and cell transplantation provided an effective strategy for improving postinfarction ventricular function.</abstract><cop>China</cop><pub>Department of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei 430022,China%Department of Cardiology,Heping Hospital,Changzhi Medical College,Changzhi,Shanxi 046000,China</pub><pmid>18304469</pmid><doi>10.1097/00029330-200802020-00013</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chinese medical journal, 2008-02, Vol.121 (4), p.347-354 |
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| subjects | Animals Blotting, Western Cells, Cultured Echocardiography Genetic Therapy Human Growth Hormone - blood Human Growth Hormone - genetics Immunohistochemistry Myoblasts, Skeletal - transplantation Myocardial Infarction - physiopathology Myocardial Infarction - therapy Rats Rats, Sprague-Dawley RNA, Messenger - analysis Transfection Ventricular Function 基因治疗 心脏功能 成肌细胞 生长激素 |
| title | Effects of transplanted myoblasts transfected with human growth hormone gene on improvement of ventricular function of rats |
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