Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver
Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in...
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| Veröffentlicht in: | Chinese medical journal 2006-03, Vol.119 (6), p.504-509 |
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| creator | Zhang, Yu-mei Chen, Xiang-mei Wu, Di Zhang, Xue-guang Lü, Yang Shi, Suo-zhu Yin, Zhong |
| description | Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice.
Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured.
Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）.
Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse in the process of aging, indicating that the oxidative level increases and the anti-oxidative ability decreases in the liver of transgenic mouse. TIMP-1 plays an important role in the process of liver aging. |
| doi_str_mv | 10.1097/00029330-200603020-00013 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_zhcmj200606010</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>21431889</cqvip_id><wanfj_id>zhcmj200606010</wanfj_id><sourcerecordid>zhcmj200606010</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-c67d63f831023eab20f9cd7b603b573ba73dd83053c0d434e69c9fd34f28ce0f3</originalsourceid><addsrcrecordid>eNpFkU1v1DAQhi0EokvhL6CIA7fA2JM48RFV5UOqxAXOluOMs14Se2snUPj1uNuFnkYaPe98vC9jFYd3HFT3HgCEQoRaAEhAEFCXFscnbCfaRtStbPhTtgOUspZKqQv2IudDEbVtJ5-zCy7bvpGN2jF9fXdMlLOPoYquWn3OG1U-7P3g15jue4tZk7-rFlrNPMdjiiv5YDLVvHCVmXyYTtJkQp4oeFstcctUzf4npZfsmTNzplfnesm-f7z-dvW5vvn66cvVh5vaNoKvtZXdKNH1yEEgmUGAU3bshvLc0HY4mA7HsUdo0cLYYENSWeVGbJzoLYHDS_b2Ye4vE5wJkz7ELYWyUf_Z2-Vw8kkCh0ewPHK7UV714rOleTaBytladr1AybsC9g-gTTHnRE4fk19M-q056PsU9L8U9P8U9CmFIn193rENC42PwrPtBXhznr2PYbotDurB2B_Oz6QFb5D3vcK_7USOWg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67823617</pqid></control><display><type>article</type><title>Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Zhang, Yu-mei ; Chen, Xiang-mei ; Wu, Di ; Zhang, Xue-guang ; Lü, Yang ; Shi, Suo-zhu ; Yin, Zhong</creator><creatorcontrib>Zhang, Yu-mei ; Chen, Xiang-mei ; Wu, Di ; Zhang, Xue-guang ; Lü, Yang ; Shi, Suo-zhu ; Yin, Zhong</creatorcontrib><description>Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice.
Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured.
Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）.
Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse in the process of aging, indicating that the oxidative level increases and the anti-oxidative ability decreases in the liver of transgenic mouse. TIMP-1 plays an important role in the process of liver aging.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><identifier>DOI: 10.1097/00029330-200603020-00013</identifier><identifier>PMID: 16584649</identifier><language>eng</language><publisher>China: Department of Nephrology, Beijing Military Command General Hospital, Beijing 100700, China%Department of Nephrology, General Hospital of PLA, Beijing 100853, China</publisher><subject>Aging - metabolism ; Animals ; Female ; Liver - metabolism ; Liver - pathology ; Male ; Matrix Metalloproteinase 2 - analysis ; Matrix Metalloproteinase 2 - genetics ; Matrix Metalloproteinase 9 - analysis ; Matrix Metalloproteinase 9 - genetics ; Mice ; Mice, Transgenic ; Monoamine Oxidase - analysis ; Reactive Oxygen Species - metabolism ; RNA, Messenger - analysis ; Superoxide Dismutase - metabolism ; Tissue Inhibitor of Metalloproteinase-1 - analysis ; Tissue Inhibitor of Metalloproteinase-1 - genetics ; 动物实验 ; 氧化作用 ; 组织抑制剂</subject><ispartof>Chinese medical journal, 2006-03, Vol.119 (6), p.504-509</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-c67d63f831023eab20f9cd7b603b573ba73dd83053c0d434e69c9fd34f28ce0f3</citedby><cites>FETCH-LOGICAL-c421t-c67d63f831023eab20f9cd7b603b573ba73dd83053c0d434e69c9fd34f28ce0f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16584649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Yu-mei</creatorcontrib><creatorcontrib>Chen, Xiang-mei</creatorcontrib><creatorcontrib>Wu, Di</creatorcontrib><creatorcontrib>Zhang, Xue-guang</creatorcontrib><creatorcontrib>Lü, Yang</creatorcontrib><creatorcontrib>Shi, Suo-zhu</creatorcontrib><creatorcontrib>Yin, Zhong</creatorcontrib><title>Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice.
Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured.
Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）.
Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse in the process of aging, indicating that the oxidative level increases and the anti-oxidative ability decreases in the liver of transgenic mouse. TIMP-1 plays an important role in the process of liver aging.</description><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Female</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Matrix Metalloproteinase 2 - analysis</subject><subject>Matrix Metalloproteinase 2 - genetics</subject><subject>Matrix Metalloproteinase 9 - analysis</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Monoamine Oxidase - analysis</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - analysis</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - genetics</subject><subject>动物实验</subject><subject>氧化作用</subject><subject>组织抑制剂</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkU1v1DAQhi0EokvhL6CIA7fA2JM48RFV5UOqxAXOluOMs14Se2snUPj1uNuFnkYaPe98vC9jFYd3HFT3HgCEQoRaAEhAEFCXFscnbCfaRtStbPhTtgOUspZKqQv2IudDEbVtJ5-zCy7bvpGN2jF9fXdMlLOPoYquWn3OG1U-7P3g15jue4tZk7-rFlrNPMdjiiv5YDLVvHCVmXyYTtJkQp4oeFstcctUzf4npZfsmTNzplfnesm-f7z-dvW5vvn66cvVh5vaNoKvtZXdKNH1yEEgmUGAU3bshvLc0HY4mA7HsUdo0cLYYENSWeVGbJzoLYHDS_b2Ye4vE5wJkz7ELYWyUf_Z2-Vw8kkCh0ewPHK7UV714rOleTaBytladr1AybsC9g-gTTHnRE4fk19M-q056PsU9L8U9P8U9CmFIn193rENC42PwrPtBXhznr2PYbotDurB2B_Oz6QFb5D3vcK_7USOWg</recordid><startdate>20060320</startdate><enddate>20060320</enddate><creator>Zhang, Yu-mei</creator><creator>Chen, Xiang-mei</creator><creator>Wu, Di</creator><creator>Zhang, Xue-guang</creator><creator>Lü, Yang</creator><creator>Shi, Suo-zhu</creator><creator>Yin, Zhong</creator><general>Department of Nephrology, Beijing Military Command General Hospital, Beijing 100700, China%Department of Nephrology, General Hospital of PLA, Beijing 100853, China</general><general>Department of Nephrology, General Hospital of PLA, Beijing 100853, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20060320</creationdate><title>Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver</title><author>Zhang, Yu-mei ; Chen, Xiang-mei ; Wu, Di ; Zhang, Xue-guang ; Lü, Yang ; Shi, Suo-zhu ; Yin, Zhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-c67d63f831023eab20f9cd7b603b573ba73dd83053c0d434e69c9fd34f28ce0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Female</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>Matrix Metalloproteinase 2 - analysis</topic><topic>Matrix Metalloproteinase 2 - genetics</topic><topic>Matrix Metalloproteinase 9 - analysis</topic><topic>Matrix Metalloproteinase 9 - genetics</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Monoamine Oxidase - analysis</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - analysis</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - genetics</topic><topic>动物实验</topic><topic>氧化作用</topic><topic>组织抑制剂</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Yu-mei</creatorcontrib><creatorcontrib>Chen, Xiang-mei</creatorcontrib><creatorcontrib>Wu, Di</creatorcontrib><creatorcontrib>Zhang, Xue-guang</creatorcontrib><creatorcontrib>Lü, Yang</creatorcontrib><creatorcontrib>Shi, Suo-zhu</creatorcontrib><creatorcontrib>Yin, Zhong</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Yu-mei</au><au>Chen, Xiang-mei</au><au>Wu, Di</au><au>Zhang, Xue-guang</au><au>Lü, Yang</au><au>Shi, Suo-zhu</au><au>Yin, Zhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2006-03-20</date><risdate>2006</risdate><volume>119</volume><issue>6</issue><spage>504</spage><epage>509</epage><pages>504-509</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background Tissue inhibitor of matrix metaUoproteinase-1 （TIMP-1） is related to the aging of many organs, but few data are available on the change of TIMP-1 in liver aging. The purpose of this study was to investigate the expression and role of TIMP-1, matrix metalloproteinase-2 （MMP-2） and MMP-9 in the process of natural aging in the livers of normal and transgenic mice, and to detect the effects of TIMP-1 on oxidative level and anti-oxidative ability of the livers of transgenic young mice.
Methods Normal and transgenic mice were divided into 3 groups according to their age： 3-month-old group （n=5）, 12-month-old group （n=5） and 24-month-old group （n=5）. Histopathological changes of the liver were observed after HE and Masson staining. The messenger RNA （mRNA） levels of TIMP-1, MMP-2 and MMP-9 were determined by semi-quantitative reverse transcriptional polymerase chain reaction; protein expression was measured by Western blot in the livers of normal and transgenic mice of various ages. Changes in levels of superoxide dismutase （SOD）, monoamine oxidase （MAO）, malondialdehyde （MDA） as well as oxidative and anti-oxidative ability were measured.
Results Histologically, more fatty degeneration and collagen deposition were found in the aging livers of transgenic mice than in those of the normal mice as their age of months increased. The mRNA and protein expressions of TIMP-1 were significantly high in the oldest animals. The histopathological changes, mRNA and protein expressions of TIMP-1 increased significantly in the liver of transgenic mice as compared with normal mice. The expression of MMP-2 and MMP-9 showed a minor change in the process of aging. Liver change and collagen deposition were not observed in young mice, but the activity of SOD decreased （P〈0.05）, and the activity of MAO （P〈0.01） and the content of MDA increased in the liver of transgenic mice （P〈0.01）.
Conclusions The expression of TIMP-1 is significantly high in the liver of transgenic mouse in the process of aging, indicating that the oxidative level increases and the anti-oxidative ability decreases in the liver of transgenic mouse. TIMP-1 plays an important role in the process of liver aging.</abstract><cop>China</cop><pub>Department of Nephrology, Beijing Military Command General Hospital, Beijing 100700, China%Department of Nephrology, General Hospital of PLA, Beijing 100853, China</pub><pmid>16584649</pmid><doi>10.1097/00029330-200603020-00013</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Chinese medical journal, 2006-03, Vol.119 (6), p.504-509 |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aging - metabolism Animals Female Liver - metabolism Liver - pathology Male Matrix Metalloproteinase 2 - analysis Matrix Metalloproteinase 2 - genetics Matrix Metalloproteinase 9 - analysis Matrix Metalloproteinase 9 - genetics Mice Mice, Transgenic Monoamine Oxidase - analysis Reactive Oxygen Species - metabolism RNA, Messenger - analysis Superoxide Dismutase - metabolism Tissue Inhibitor of Metalloproteinase-1 - analysis Tissue Inhibitor of Metalloproteinase-1 - genetics 动物实验 氧化作用 组织抑制剂 |
| title | Expression of tissue inhibitor of matrix metalloproteinase-1 in aging of transgenic mouse liver |
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