A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation
Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/recep...
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| Veröffentlicht in: | Chinese medical journal 2006-03, Vol.119 (6), p.496-503 |
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| creator | Liu, Feng Guo, Xi-rong Gong, Hai-xia Ni, Yu-hui Fei, Li Pan, Xiao-qin Guo, Mei Chen, Rong-hua |
| description | Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor.
Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide.
Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation.
Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte. |
| doi_str_mv | 10.1097/00029330-200603020-00011 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_zhcmj200606009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>21431887</cqvip_id><wanfj_id>zhcmj200606009</wanfj_id><sourcerecordid>zhcmj200606009</sourcerecordid><originalsourceid>FETCH-LOGICAL-c421t-3a8e8f4abdd1cdf01b03b476b548dda5ceef387289f9338659fecb7e7b76cad23</originalsourceid><addsrcrecordid>eNpFkUtvFDEQhK2IKNkE_gKyOHAb8GtszzGKCCCtxCU5W360d73Meia2V2jz65kkCzm11PqqWl2FEKbkCyWD-koIYQPnpGOESMIJI92yovQMrVgvWNdLQd-hFeFSdnIYhkt0VetuEfW9khfokspeCyn0CtkbXKCm2lLGLuWQ8gbPMLcUAFcYwTcI2B3xvLUbwCHVebRHnPI2udQq5ve8W1M8F7AhzZM_tmcoRiiQW7ItTfk9Oo92rPDhNK_Rw923-9sf3frX95-3N-vOC0Zbx60GHYV1IVAfIqGOcCeUdL3QIdjeA0SuFdNDXD7Xsh8ieKdAOSW9DYxfo8-vvn9sjjZvzG46lLxcNE9bv9-9JCUJGd7AuUyPB6jN7FP1MI42w3SoRirNhKJ8AfUr6MtUa4Fo5pL2thwNJea5B_OvB_O_B_PSwyL9eLpxcHsIb8JT8Avw6eS9nfLmcYndOOt_xzSCYVRwqrXifwEQPY9Z</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67824713</pqid></control><display><type>article</type><title>A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Liu, Feng ; Guo, Xi-rong ; Gong, Hai-xia ; Ni, Yu-hui ; Fei, Li ; Pan, Xiao-qin ; Guo, Mei ; Chen, Rong-hua</creator><creatorcontrib>Liu, Feng ; Guo, Xi-rong ; Gong, Hai-xia ; Ni, Yu-hui ; Fei, Li ; Pan, Xiao-qin ; Guo, Mei ; Chen, Rong-hua</creatorcontrib><description>Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor.
Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide.
Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation.
Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><identifier>DOI: 10.1097/00029330-200603020-00011</identifier><identifier>PMID: 16584648</identifier><language>eng</language><publisher>China: Department of Pediatrics, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China%Department of Pediatrics, Nanjing Maternity & Child Health Hospital of Nanjing Medical University, Nanjing 210029, China%Department of Pediatrics, Center of Human Functional Genomics, Nanjing Medical University, Nanjing 210029, China</publisher><subject>3T3-L1 Cells ; Adipocytes - cytology ; Adipocytes - drug effects ; Amino Acid Sequence ; Animals ; Base Sequence ; Carrier Proteins - isolation & purification ; Carrier Proteins - pharmacology ; Cell Differentiation - drug effects ; Mice ; Molecular Sequence Data ; Peptide Library ; Rats ; Resistin - antagonists & inhibitors ; Resistin - metabolism ; 前成脂肪细胞 ; 抗菌素 ; 缩氨酸 ; 锰铜电阻合金</subject><ispartof>Chinese medical journal, 2006-03, Vol.119 (6), p.496-503</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-3a8e8f4abdd1cdf01b03b476b548dda5ceef387289f9338659fecb7e7b76cad23</citedby><cites>FETCH-LOGICAL-c421t-3a8e8f4abdd1cdf01b03b476b548dda5ceef387289f9338659fecb7e7b76cad23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16584648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Feng</creatorcontrib><creatorcontrib>Guo, Xi-rong</creatorcontrib><creatorcontrib>Gong, Hai-xia</creatorcontrib><creatorcontrib>Ni, Yu-hui</creatorcontrib><creatorcontrib>Fei, Li</creatorcontrib><creatorcontrib>Pan, Xiao-qin</creatorcontrib><creatorcontrib>Guo, Mei</creatorcontrib><creatorcontrib>Chen, Rong-hua</creatorcontrib><title>A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor.
Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide.
Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation.
Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.</description><subject>3T3-L1 Cells</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Carrier Proteins - isolation & purification</subject><subject>Carrier Proteins - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Peptide Library</subject><subject>Rats</subject><subject>Resistin - antagonists & inhibitors</subject><subject>Resistin - metabolism</subject><subject>前成脂肪细胞</subject><subject>抗菌素</subject><subject>缩氨酸</subject><subject>锰铜电阻合金</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkUtvFDEQhK2IKNkE_gKyOHAb8GtszzGKCCCtxCU5W360d73Meia2V2jz65kkCzm11PqqWl2FEKbkCyWD-koIYQPnpGOESMIJI92yovQMrVgvWNdLQd-hFeFSdnIYhkt0VetuEfW9khfokspeCyn0CtkbXKCm2lLGLuWQ8gbPMLcUAFcYwTcI2B3xvLUbwCHVebRHnPI2udQq5ve8W1M8F7AhzZM_tmcoRiiQW7ItTfk9Oo92rPDhNK_Rw923-9sf3frX95-3N-vOC0Zbx60GHYV1IVAfIqGOcCeUdL3QIdjeA0SuFdNDXD7Xsh8ieKdAOSW9DYxfo8-vvn9sjjZvzG46lLxcNE9bv9-9JCUJGd7AuUyPB6jN7FP1MI42w3SoRirNhKJ8AfUr6MtUa4Fo5pL2thwNJea5B_OvB_O_B_PSwyL9eLpxcHsIb8JT8Avw6eS9nfLmcYndOOt_xzSCYVRwqrXifwEQPY9Z</recordid><startdate>20060320</startdate><enddate>20060320</enddate><creator>Liu, Feng</creator><creator>Guo, Xi-rong</creator><creator>Gong, Hai-xia</creator><creator>Ni, Yu-hui</creator><creator>Fei, Li</creator><creator>Pan, Xiao-qin</creator><creator>Guo, Mei</creator><creator>Chen, Rong-hua</creator><general>Department of Pediatrics, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China%Department of Pediatrics, Nanjing Maternity & Child Health Hospital of Nanjing Medical University, Nanjing 210029, China%Department of Pediatrics, Center of Human Functional Genomics, Nanjing Medical University, Nanjing 210029, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20060320</creationdate><title>A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation</title><author>Liu, Feng ; Guo, Xi-rong ; Gong, Hai-xia ; Ni, Yu-hui ; Fei, Li ; Pan, Xiao-qin ; Guo, Mei ; Chen, Rong-hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-3a8e8f4abdd1cdf01b03b476b548dda5ceef387289f9338659fecb7e7b76cad23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>3T3-L1 Cells</topic><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Carrier Proteins - isolation & purification</topic><topic>Carrier Proteins - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Peptide Library</topic><topic>Rats</topic><topic>Resistin - antagonists & inhibitors</topic><topic>Resistin - metabolism</topic><topic>前成脂肪细胞</topic><topic>抗菌素</topic><topic>缩氨酸</topic><topic>锰铜电阻合金</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Feng</creatorcontrib><creatorcontrib>Guo, Xi-rong</creatorcontrib><creatorcontrib>Gong, Hai-xia</creatorcontrib><creatorcontrib>Ni, Yu-hui</creatorcontrib><creatorcontrib>Fei, Li</creatorcontrib><creatorcontrib>Pan, Xiao-qin</creatorcontrib><creatorcontrib>Guo, Mei</creatorcontrib><creatorcontrib>Chen, Rong-hua</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Feng</au><au>Guo, Xi-rong</au><au>Gong, Hai-xia</au><au>Ni, Yu-hui</au><au>Fei, Li</au><au>Pan, Xiao-qin</au><au>Guo, Mei</au><au>Chen, Rong-hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2006-03-20</date><risdate>2006</risdate><volume>119</volume><issue>6</issue><spage>496</spage><epage>503</epage><pages>496-503</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background Resistin, a newly discovered cysteine-rich hormone secreted mainly by adipose tissues, has been proposed to form a biochemical link between obesity and type 2 diabetes. However, the resistin receptor has not yet been identified. This study aimed to identify resistin binding proteins/receptor.
Methods Three cDNA fragments with the same 11 bp 5＇ sequence were found by screening a cDNA phage display library of rat multiple tissues. As the reading frames of the same 11 bp 5＇ sequence were interrupted by a TGA stop codon, plaque lift assay was consequently used to prove the readthrough phenomenon. The stop codon in the same 11 bp 5＇ sequence was replaced by tryptophan, and the binding activity of the coded peptide [AWIL, which was designated as resistin binding peptide （RBP）] with resistin was identified by the confocal microscopy technique and the affinity chromatography experiment, pDual GC-resistin and pDual GC-resistin binding peptide were co-transfected into 3T3-L1 cells to confirm the function of resistin binding peptide.
Results Three cDNA fragments with the same 11 bp 5＇ sequence were found. The TGA stop codon in reading frames of the same 11 bp 5＇ sequence was proved to be readthroughed. The binding activity of RBP with resistin was consequently identified. The expression of the resistin binding peptide in 3T3-L1 preadipocytes expressing pDual G-C-resistin significantly inhibited the adipogenic differentiation.
Conclusion RBP could effectively rescue the promoted differentiation of resistin overxepressed 3T3-L1 preadipocyte.</abstract><cop>China</cop><pub>Department of Pediatrics, Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011, China%Department of Pediatrics, Nanjing Maternity & Child Health Hospital of Nanjing Medical University, Nanjing 210029, China%Department of Pediatrics, Center of Human Functional Genomics, Nanjing Medical University, Nanjing 210029, China</pub><pmid>16584648</pmid><doi>10.1097/00029330-200603020-00011</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3-L1 Cells Adipocytes - cytology Adipocytes - drug effects Amino Acid Sequence Animals Base Sequence Carrier Proteins - isolation & purification Carrier Proteins - pharmacology Cell Differentiation - drug effects Mice Molecular Sequence Data Peptide Library Rats Resistin - antagonists & inhibitors Resistin - metabolism 前成脂肪细胞 抗菌素 缩氨酸 锰铜电阻合金 |
| title | A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation |
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