Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice
We identified a leafy head mutant plal-5 （plastochron 1-5） from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γ ray irradiation. The plal-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, plal-5 has more leaves and fewer tillers, and it fails to produ...
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| Veröffentlicht in: | Rice science 2013-09, Vol.20 (5), p.329-335 |
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| creator | FENG, Gong-neng ZHANG, Chang-quan ZHAO, Dong-sheng ZHU, Kong-zhi TU, Huai-zhou XU, Chen-wu LIU, Qiao-quan |
| description | We identified a leafy head mutant plal-5 （plastochron 1-5） from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γ ray irradiation. The plal-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, plal-5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the plal-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the plal-5 mutant, which might result in a downstream frame shift and premature termination. These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5. |
| doi_str_mv | 10.1016/S1672-6308(13)60140-7 |
| format | Article |
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The plal-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, plal-5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the plal-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the plal-5 mutant, which might result in a downstream frame shift and premature termination. These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.</description><identifier>ISSN: 1672-6308</identifier><identifier>EISSN: 1876-4762</identifier><identifier>DOI: 10.1016/S1672-6308(13)60140-7</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>chromosomes ; cultivars ; cytochrome P-450 ; exons ; gene cloning ; genes ; genetic analysis ; genetic markers ; inflorescences ; irradiation ; leafy head mutant ; leaves ; maturity stage ; microsatellite repeats ; molecular cloning ; mutants ; Oryza sativa ; P450 CYP78A11 gene ; phenotype ; progeny ; rice ; sequence analysis ; tillers ; 叶球 ; 图位克隆 ; 水稻 ; 目标基因 ; 突变基因 ; 简单重复序列 ; 精细定位 ; 细胞色素P450</subject><ispartof>Rice science, 2013-09, Vol.20 (5), p.329-335</ispartof><rights>2013 China National Rice Research Institute</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-bb2ad45cad8dec6b1543556b7c6537d3c3ce4cb1971ef93fd5fd6924959875023</citedby><cites>FETCH-LOGICAL-c395t-bb2ad45cad8dec6b1543556b7c6537d3c3ce4cb1971ef93fd5fd6924959875023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84241A/84241A.jpg</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids></links><search><creatorcontrib>FENG, Gong-neng</creatorcontrib><creatorcontrib>ZHANG, Chang-quan</creatorcontrib><creatorcontrib>ZHAO, Dong-sheng</creatorcontrib><creatorcontrib>ZHU, Kong-zhi</creatorcontrib><creatorcontrib>TU, Huai-zhou</creatorcontrib><creatorcontrib>XU, Chen-wu</creatorcontrib><creatorcontrib>LIU, Qiao-quan</creatorcontrib><title>Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice</title><title>Rice science</title><addtitle>Rice science</addtitle><description>We identified a leafy head mutant plal-5 （plastochron 1-5） from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γ ray irradiation. The plal-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, plal-5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the plal-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the plal-5 mutant, which might result in a downstream frame shift and premature termination. These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.</description><subject>chromosomes</subject><subject>cultivars</subject><subject>cytochrome P-450</subject><subject>exons</subject><subject>gene cloning</subject><subject>genes</subject><subject>genetic analysis</subject><subject>genetic markers</subject><subject>inflorescences</subject><subject>irradiation</subject><subject>leafy head mutant</subject><subject>leaves</subject><subject>maturity stage</subject><subject>microsatellite repeats</subject><subject>molecular cloning</subject><subject>mutants</subject><subject>Oryza sativa</subject><subject>P450 CYP78A11 gene</subject><subject>phenotype</subject><subject>progeny</subject><subject>rice</subject><subject>sequence analysis</subject><subject>tillers</subject><subject>叶球</subject><subject>图位克隆</subject><subject>水稻</subject><subject>目标基因</subject><subject>突变基因</subject><subject>简单重复序列</subject><subject>精细定位</subject><subject>细胞色素P450</subject><issn>1672-6308</issn><issn>1876-4762</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkMtOwzAQRS0EEqXwCQizA6GAHb-SFaoq2iK1QqJ0bTl-BFfFKUkKKl-P2wBbVjOLM3dmDgDnGN1ihPndHHORJpyg7AqTa44wRYk4AD2cCZ5QwdPD2P8ix-CkaZYIccpR3gODkQ8WztR67UMJVTBwuKrCrq8cnFrltnBilYGzTatCC8c20uuVwgmDPsBnr-0pOHJq1dizn9oHi9HDy3CSTJ_Gj8PBNNEkZ21SFKkylGllMmM1LzCjhDFeCM0ZEYZooi3VBc4Fti4nzjBneJ7SnOWZYCglfXDT5X6q4FQo5bLa1CFulF9lY7bLtpA2RZgghhCJNOtoXVdNU1sn17V_U_VWYiR31uTemtwpkZjIvTUp4txFN-dUJVVZ-0Yu5jE2hiKW0ZjfB_cdYeOzH97WstHeBm2Nr61upan8vzsuf257rUL5HmX_HUcFyfL4M_kGrnGF_Q</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>FENG, Gong-neng</creator><creator>ZHANG, Chang-quan</creator><creator>ZHAO, Dong-sheng</creator><creator>ZHU, Kong-zhi</creator><creator>TU, Huai-zhou</creator><creator>XU, Chen-wu</creator><creator>LIU, Qiao-quan</creator><general>Elsevier B.V</general><general>yancheng Institute of Technology, Yancheng 224051, China%Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China</general><general>Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W95</scope><scope>~WA</scope><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20130901</creationdate><title>Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice</title><author>FENG, Gong-neng ; ZHANG, Chang-quan ; ZHAO, Dong-sheng ; ZHU, Kong-zhi ; TU, Huai-zhou ; XU, Chen-wu ; LIU, Qiao-quan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-bb2ad45cad8dec6b1543556b7c6537d3c3ce4cb1971ef93fd5fd6924959875023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>chromosomes</topic><topic>cultivars</topic><topic>cytochrome P-450</topic><topic>exons</topic><topic>gene cloning</topic><topic>genes</topic><topic>genetic analysis</topic><topic>genetic markers</topic><topic>inflorescences</topic><topic>irradiation</topic><topic>leafy head mutant</topic><topic>leaves</topic><topic>maturity stage</topic><topic>microsatellite repeats</topic><topic>molecular cloning</topic><topic>mutants</topic><topic>Oryza sativa</topic><topic>P450 CYP78A11 gene</topic><topic>phenotype</topic><topic>progeny</topic><topic>rice</topic><topic>sequence analysis</topic><topic>tillers</topic><topic>叶球</topic><topic>图位克隆</topic><topic>水稻</topic><topic>目标基因</topic><topic>突变基因</topic><topic>简单重复序列</topic><topic>精细定位</topic><topic>细胞色素P450</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>FENG, Gong-neng</creatorcontrib><creatorcontrib>ZHANG, Chang-quan</creatorcontrib><creatorcontrib>ZHAO, Dong-sheng</creatorcontrib><creatorcontrib>ZHU, Kong-zhi</creatorcontrib><creatorcontrib>TU, Huai-zhou</creatorcontrib><creatorcontrib>XU, Chen-wu</creatorcontrib><creatorcontrib>LIU, Qiao-quan</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-农业科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>AGRIS</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Rice science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FENG, Gong-neng</au><au>ZHANG, Chang-quan</au><au>ZHAO, Dong-sheng</au><au>ZHU, Kong-zhi</au><au>TU, Huai-zhou</au><au>XU, Chen-wu</au><au>LIU, Qiao-quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice</atitle><jtitle>Rice science</jtitle><addtitle>Rice science</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>20</volume><issue>5</issue><spage>329</spage><epage>335</epage><pages>329-335</pages><issn>1672-6308</issn><eissn>1876-4762</eissn><abstract>We identified a leafy head mutant plal-5 （plastochron 1-5） from the progeny of japonica rice cultivar Taipei 309 treated with 60Co-γ ray irradiation. The plal-5 mutant has a dwarf phenotype and small leaves. Compared with its wild type, plal-5 has more leaves and fewer tillers, and it fails to produce normal panicles at the maturity stage. Genetic analysis showed that the plal-5 phenotype is controlled by a single recessive nuclear gene. Using the map-based cloning strategy, we narrowed down the location of the target gene to a 58-kb region between simple sequence repeat markers CHR1027 and CHR1030 on the long arm of chromosome 10. The target gene cosegregated with molecular markers CHR1028 and CHR1029. There were five predicted genes in the mapped region. The results from sequencing analysis revealed that there was one base deletion in the first exon of LOC_Os10g26340 encoding cytochrome P450 CYP78A11 in the plal-5 mutant, which might result in a downstream frame shift and premature termination. These results suggest that the P450 CYP78A11 gene is the candidate gene of PLA1-5.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S1672-6308(13)60140-7</doi><tpages>7</tpages></addata></record> |
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| ispartof | Rice science, 2013-09, Vol.20 (5), p.329-335 |
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| subjects | chromosomes cultivars cytochrome P-450 exons gene cloning genes genetic analysis genetic markers inflorescences irradiation leafy head mutant leaves maturity stage microsatellite repeats molecular cloning mutants Oryza sativa P450 CYP78A11 gene phenotype progeny rice sequence analysis tillers 叶球 图位克隆 水稻 目标基因 突变基因 简单重复序列 精细定位 细胞色素P450 |
| title | Fine Mapping and Cloning of Leafy Head Mutant Gene pla1-5 in Rice |
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