Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice
Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with...
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| Veröffentlicht in: | Rice science 2009-06, Vol.16 (2), p.157-160 |
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| creator | Fang, Yuan Xu, Li-hui Tian, Wen-xiao Huai, Yan Yu, Shan-hong Lou, Miao-miao Xie, Guan-lin |
| description | Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1：100. |
| doi_str_mv | 10.1016/S1672-6308(08)60073-6 |
| format | Article |
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It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1：100.</description><identifier>ISSN: 1672-6308</identifier><identifier>EISSN: 1876-4762</identifier><identifier>DOI: 10.1016/S1672-6308(08)60073-6</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>bacterial grain rot ; Burkholderia glumae ; detection ; PCR扩增法 ; PCR方法 ; real-time fluorescence polymerase chain reaction ; rice ; 实时PCR ; 实时荧光PCR检测方法 ; 探测效率 ; 有效管理 ; 种子检疫</subject><ispartof>Rice science, 2009-06, Vol.16 (2), p.157-160</ispartof><rights>2009 China National Rice Research Institute</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-593895c06553b242f75fe11647efe709bff62847b7a7edb6908fc90e8acf682b3</citedby><cites>FETCH-LOGICAL-c372t-593895c06553b242f75fe11647efe709bff62847b7a7edb6908fc90e8acf682b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84241A/84241A.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Fang, Yuan</creatorcontrib><creatorcontrib>Xu, Li-hui</creatorcontrib><creatorcontrib>Tian, Wen-xiao</creatorcontrib><creatorcontrib>Huai, Yan</creatorcontrib><creatorcontrib>Yu, Shan-hong</creatorcontrib><creatorcontrib>Lou, Miao-miao</creatorcontrib><creatorcontrib>Xie, Guan-lin</creatorcontrib><title>Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice</title><title>Rice science</title><addtitle>Rice science</addtitle><description>Burkholderia glumae causing seedling rot and grain rot of rice was listed as a plant quarantine disease of China in 2007. It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. 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It＇s quite necessary to set up effective detection methods for the pathogen to manage further dispersal of this disease. The present study combined the real-time PCR method with classical PCR to increase the detecting efficiency, and to develop an accurate, rapid and sensitive method to detect the pathogen in the seed quarantine for effective management of the disease. The results showed that all the tested strains of B. glumae produced about 139 bp specific fragments by the real-time PCR and the general PCR methods, while others showed negative PCR result. The bacteria could be detected at the concentrations of 1×10^4 CFU/mL by general PCR method and at the concentrations below 100 CFU/mL by real-time fluorescence PCR method. B. glumae could be detected when the inoculated and healthy seeds were mixed with a proportion of 1：100.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S1672-6308(08)60073-6</doi><tpages>4</tpages></addata></record> |
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| ispartof | Rice science, 2009-06, Vol.16 (2), p.157-160 |
| issn | 1672-6308 1876-4762 |
| language | eng |
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| source | Alma/SFX Local Collection |
| subjects | bacterial grain rot Burkholderia glumae detection PCR扩增法 PCR方法 real-time fluorescence polymerase chain reaction rice 实时PCR 实时荧光PCR检测方法 探测效率 有效管理 种子检疫 |
| title | Real-time Fluorescence PCR Method for Detection of Burkholderia glumae from Rice |
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