Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella
Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic selection. The heterophils/lymphocytes (H/L) ratio in the blood reflects the immune system status of chicken. We compared the genome data and spleen transcriptomes between the H/L...
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| Veröffentlicht in: | Journal of Integrative Agriculture 2022-08, Vol.21 (8), p.2372-2383 |
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| container_title | Journal of Integrative Agriculture |
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| creator | WANG, Jie ZHANG, Qi SÁNCHEZ, Astrid Lissette BARRETO ZHU, Bo WANG, Qiao ZHENG, Mai-qing LI, Qing-he CUI, Huan-xian WEN, Jie ZHAO, Gui-ping |
| description | Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic selection. The heterophils/lymphocytes (H/L) ratio in the blood reflects the immune system status of chicken. We compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens, after Salmonella infection, aiming to identify the key genes participating in the antibacterial activity in the spleen. The results revealed that, the selected population had stronger (P |
| doi_str_mv | 10.1016/S2095-3119(21)63770-X |
| format | Article |
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The heterophils/lymphocytes (H/L) ratio in the blood reflects the immune system status of chicken. We compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens, after Salmonella infection, aiming to identify the key genes participating in the antibacterial activity in the spleen. The results revealed that, the selected population had stronger (P<0.05) liver resistance to Salmonella typhimurium (ST) than the non-selected population. In the selected and non-selected lines, the identified differentiation genes encode proteins involved in biological processes or metabolic pathways that included the TGF-beta signaling pathway, FoxO signaling pathway, and Salmonella infection pathway. The results of the analysis of all identified differentially expressed genes (DEGs) of spleen revealed that the G protein-coupled receptor (GPCR) and insulin-like growth factor (IGF-I) signaling pathways were involved in the Salmonella infection pathway. Integrated analysis of DEGs and FST (fixation index), identified candidate genes involved in Salmonella infection pathway, such as GPR39, NTRK2, and ANXA1. The extensive genomic changes highlight the polygenic genetic of the immune response in these chicken populations. Numerous genes related to the immune performance are differentially expressed in the selected and non-selected lines and the selected lines has a higher resistance to Salmonella.</description><identifier>ISSN: 2095-3119</identifier><identifier>EISSN: 2352-3425</identifier><identifier>DOI: 10.1016/S2095-3119(21)63770-X</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>disease-resistance ; heterophils/lymphocytes ; RNA-seq ; Salmonella typhimurium ; selective sweep</subject><ispartof>Journal of Integrative Agriculture, 2022-08, Vol.21 (8), p.2372-2383</ispartof><rights>2022 CAAS. Publishing services by Elsevier B.V</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-9500231c95d08f45ff7aa9ca17e36b4f0c1c2a6a576b416f6ecf8bc942b0d5563</citedby><cites>FETCH-LOGICAL-c390t-9500231c95d08f45ff7aa9ca17e36b4f0c1c2a6a576b416f6ecf8bc942b0d5563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgnykx-e/zgnykx-e.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S2095-3119(21)63770-X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>WANG, Jie</creatorcontrib><creatorcontrib>ZHANG, Qi</creatorcontrib><creatorcontrib>SÁNCHEZ, Astrid Lissette BARRETO</creatorcontrib><creatorcontrib>ZHU, Bo</creatorcontrib><creatorcontrib>WANG, Qiao</creatorcontrib><creatorcontrib>ZHENG, Mai-qing</creatorcontrib><creatorcontrib>LI, Qing-he</creatorcontrib><creatorcontrib>CUI, Huan-xian</creatorcontrib><creatorcontrib>WEN, Jie</creatorcontrib><creatorcontrib>ZHAO, Gui-ping</creatorcontrib><title>Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella</title><title>Journal of Integrative Agriculture</title><description>Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic selection. The heterophils/lymphocytes (H/L) ratio in the blood reflects the immune system status of chicken. We compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens, after Salmonella infection, aiming to identify the key genes participating in the antibacterial activity in the spleen. The results revealed that, the selected population had stronger (P<0.05) liver resistance to Salmonella typhimurium (ST) than the non-selected population. In the selected and non-selected lines, the identified differentiation genes encode proteins involved in biological processes or metabolic pathways that included the TGF-beta signaling pathway, FoxO signaling pathway, and Salmonella infection pathway. The results of the analysis of all identified differentially expressed genes (DEGs) of spleen revealed that the G protein-coupled receptor (GPCR) and insulin-like growth factor (IGF-I) signaling pathways were involved in the Salmonella infection pathway. Integrated analysis of DEGs and FST (fixation index), identified candidate genes involved in Salmonella infection pathway, such as GPR39, NTRK2, and ANXA1. The extensive genomic changes highlight the polygenic genetic of the immune response in these chicken populations. Numerous genes related to the immune performance are differentially expressed in the selected and non-selected lines and the selected lines has a higher resistance to Salmonella.</description><subject>disease-resistance</subject><subject>heterophils/lymphocytes</subject><subject>RNA-seq</subject><subject>Salmonella typhimurium</subject><subject>selective sweep</subject><issn>2095-3119</issn><issn>2352-3425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFUU1P3TAQjCoqFQE_oZKP5RC6dp6Tl1NVodIiIXGAStysfc6aGBw7sl0g_Jb-2Dq89tzTfmh2VjNTVR85nHHg7ecbAb2sG877T4Kftk3XQX33rjoUjRR1sxHyoPT_IB-qk5QeAIBLCdBuD6vftxF90tHOOUzE0KNbkk0sGJZHYml2RH6dRsoUwzxal1gOzC3TPAa9ZEosYrahTuRIZxqYHq1-JF_29EToyqYw2cgm0iN6m6aVbrDGUCSfLboCLC8zek0r9Q26KXhyDo-r9wZdopO_9aj6efHt9vxHfXX9_fL861Wtmx5y3RctouG6lwNszUYa0yH2GnlHTbvbGNBcC2xRdmXirWlJm-1O9xuxg0HKtjmqTve8z-gN-nv1EH7F4kRSr_d-eXxRJEAI2ALvClbusTqGlCIZNUc7YVwUB7Umot4SUavdSnD1loi6K3df9ndUhDxZiippS0XyYGPxTQ3B_ofhD3-wl-s</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>WANG, Jie</creator><creator>ZHANG, Qi</creator><creator>SÁNCHEZ, Astrid Lissette BARRETO</creator><creator>ZHU, Bo</creator><creator>WANG, Qiao</creator><creator>ZHENG, Mai-qing</creator><creator>LI, Qing-he</creator><creator>CUI, Huan-xian</creator><creator>WEN, Jie</creator><creator>ZHAO, Gui-ping</creator><general>Elsevier B.V</general><general>State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20220801</creationdate><title>Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella</title><author>WANG, Jie ; ZHANG, Qi ; SÁNCHEZ, Astrid Lissette BARRETO ; ZHU, Bo ; WANG, Qiao ; ZHENG, Mai-qing ; LI, Qing-he ; CUI, Huan-xian ; WEN, Jie ; ZHAO, Gui-ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-9500231c95d08f45ff7aa9ca17e36b4f0c1c2a6a576b416f6ecf8bc942b0d5563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>disease-resistance</topic><topic>heterophils/lymphocytes</topic><topic>RNA-seq</topic><topic>Salmonella typhimurium</topic><topic>selective sweep</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WANG, Jie</creatorcontrib><creatorcontrib>ZHANG, Qi</creatorcontrib><creatorcontrib>SÁNCHEZ, Astrid Lissette BARRETO</creatorcontrib><creatorcontrib>ZHU, Bo</creatorcontrib><creatorcontrib>WANG, Qiao</creatorcontrib><creatorcontrib>ZHENG, Mai-qing</creatorcontrib><creatorcontrib>LI, Qing-he</creatorcontrib><creatorcontrib>CUI, Huan-xian</creatorcontrib><creatorcontrib>WEN, Jie</creatorcontrib><creatorcontrib>ZHAO, Gui-ping</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Integrative Agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WANG, Jie</au><au>ZHANG, Qi</au><au>SÁNCHEZ, Astrid Lissette BARRETO</au><au>ZHU, Bo</au><au>WANG, Qiao</au><au>ZHENG, Mai-qing</au><au>LI, Qing-he</au><au>CUI, Huan-xian</au><au>WEN, Jie</au><au>ZHAO, Gui-ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella</atitle><jtitle>Journal of Integrative Agriculture</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>21</volume><issue>8</issue><spage>2372</spage><epage>2383</epage><pages>2372-2383</pages><issn>2095-3119</issn><eissn>2352-3425</eissn><abstract>Salmonella is one of the most common food-borne pathogens and its resistance in chicken can be improved through genetic selection. The heterophils/lymphocytes (H/L) ratio in the blood reflects the immune system status of chicken. We compared the genome data and spleen transcriptomes between the H/L ratio-selected and non-selected chickens, after Salmonella infection, aiming to identify the key genes participating in the antibacterial activity in the spleen. The results revealed that, the selected population had stronger (P<0.05) liver resistance to Salmonella typhimurium (ST) than the non-selected population. In the selected and non-selected lines, the identified differentiation genes encode proteins involved in biological processes or metabolic pathways that included the TGF-beta signaling pathway, FoxO signaling pathway, and Salmonella infection pathway. The results of the analysis of all identified differentially expressed genes (DEGs) of spleen revealed that the G protein-coupled receptor (GPCR) and insulin-like growth factor (IGF-I) signaling pathways were involved in the Salmonella infection pathway. Integrated analysis of DEGs and FST (fixation index), identified candidate genes involved in Salmonella infection pathway, such as GPR39, NTRK2, and ANXA1. The extensive genomic changes highlight the polygenic genetic of the immune response in these chicken populations. Numerous genes related to the immune performance are differentially expressed in the selected and non-selected lines and the selected lines has a higher resistance to Salmonella.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S2095-3119(21)63770-X</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | disease-resistance heterophils/lymphocytes RNA-seq Salmonella typhimurium selective sweep |
| title | Transcriptome analysis of the spleen of heterophils to lymphocytes ratio-selected chickens revealed their mechanism of differential resistance to Salmonella |
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