PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene
Perilipin1 (PLIN1) is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets (LDs) in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol (TG). Mammalian studies have shown that Plin1 gene transcription is mainly...
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| Veröffentlicht in: | Journal of Integrative Agriculture 2022-08, Vol.21 (8), p.2357-2371 |
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| container_title | Journal of Integrative Agriculture |
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| creator | SUN, Yu-hang ZHAI, Gui-ying PANG, Yong-jia LI, Rui LI, Yu-mao CAO, Zhi-ping WANG, Ning LI, Hui WANG, Yu-xiang |
| description | Perilipin1 (PLIN1) is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets (LDs) in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol (TG). Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptor-gamma (PPARγ), the master regulator of adipogenesis. However, the regulatory mechanism of the chicken Plin1 (cPlin1) gene is poorly understood. The present study aimed to investigate whether Plin1 is regulated by PPARγ in chickens and identify its exact molecular mechanism. Reporter gene and expression assays showed that PPARγ2, but not PPARγ1, activated (P |
| doi_str_mv | 10.1016/S2095-3119(21)63896-0 |
| format | Article |
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Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptor-gamma (PPARγ), the master regulator of adipogenesis. However, the regulatory mechanism of the chicken Plin1 (cPlin1) gene is poorly understood. The present study aimed to investigate whether Plin1 is regulated by PPARγ in chickens and identify its exact molecular mechanism. Reporter gene and expression assays showed that PPARγ2, but not PPARγ1, activated (P<0.01) the cPlin1 gene promoter. An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression. In summary, our results show that PPARγ promotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.</description><identifier>ISSN: 2095-3119</identifier><identifier>EISSN: 2352-3425</identifier><identifier>DOI: 10.1016/S2095-3119(21)63896-0</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>chicken ; isoform ; Plin1 ; PPAR gamma ; transcriptional regulation</subject><ispartof>Journal of Integrative Agriculture, 2022-08, Vol.21 (8), p.2357-2371</ispartof><rights>2022 CAAS. Publishing services by Elsevier B.V</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-195692abd883ac4f4812ed75dedf2fc8219d2b1ba2a7bb70b9da68ded522a263</citedby><cites>FETCH-LOGICAL-c390t-195692abd883ac4f4812ed75dedf2fc8219d2b1ba2a7bb70b9da68ded522a263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgnykx-e/zgnykx-e.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S2095-3119(21)63896-0$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,860,3536,27903,27904,45974</link.rule.ids></links><search><creatorcontrib>SUN, Yu-hang</creatorcontrib><creatorcontrib>ZHAI, Gui-ying</creatorcontrib><creatorcontrib>PANG, Yong-jia</creatorcontrib><creatorcontrib>LI, Rui</creatorcontrib><creatorcontrib>LI, Yu-mao</creatorcontrib><creatorcontrib>CAO, Zhi-ping</creatorcontrib><creatorcontrib>WANG, Ning</creatorcontrib><creatorcontrib>LI, Hui</creatorcontrib><creatorcontrib>WANG, Yu-xiang</creatorcontrib><title>PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene</title><title>Journal of Integrative Agriculture</title><description>Perilipin1 (PLIN1) is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets (LDs) in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol (TG). Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptor-gamma (PPARγ), the master regulator of adipogenesis. However, the regulatory mechanism of the chicken Plin1 (cPlin1) gene is poorly understood. The present study aimed to investigate whether Plin1 is regulated by PPARγ in chickens and identify its exact molecular mechanism. Reporter gene and expression assays showed that PPARγ2, but not PPARγ1, activated (P<0.01) the cPlin1 gene promoter. An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression. In summary, our results show that PPARγ promotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.</description><subject>chicken</subject><subject>isoform</subject><subject>Plin1</subject><subject>PPAR gamma</subject><subject>transcriptional regulation</subject><issn>2095-3119</issn><issn>2352-3425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkE1OwzAQhSMEEhX0CEhe0kXAntRpzAZVFX9SJSro3nKcSeo2cSo7LS3X4h6ciaSFNasZzXtvRvMFwRWjN4yy-PYdqOBhxJi4BjaIo0TEIT0JehBxCKMh8NO2_7OcB33vl5RSxjmlcdILlrPZ-I0UqqoU3JH5AkmljCXG13ntKlLnpDN8f5FmoRqyrr1pzBbLPXFYbErVoG8VJLhbO_Te1LaLdBO9MHqFlsxKYxkp0OJlcJar0mP_t14E88eH-eQ5nL4-vUzG01BHgjYhEzwWoNIsSSKlh_kwYYDZiGeY5ZDrBJjIIGWpAjVK0xFNRabipFU5gII4uggGx7UfyubKFnJZb5xtD8rPwu5XO4lAAWjSwmu9_OjVrvbeYS7XzlTK7SWjssMrD3hlx04Ckwe8kra5-2MO2z-2Bp302qDVmBmHupFZbf7Z8APO24K6</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>SUN, Yu-hang</creator><creator>ZHAI, Gui-ying</creator><creator>PANG, Yong-jia</creator><creator>LI, Rui</creator><creator>LI, Yu-mao</creator><creator>CAO, Zhi-ping</creator><creator>WANG, Ning</creator><creator>LI, Hui</creator><creator>WANG, Yu-xiang</creator><general>Elsevier B.V</general><general>Key Laboratory of Chicken Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Harbin 150030,P.R.China</general><general>Fujian Sunnzer Biotechnology Development Co.,Ltd.,Guangze 354100,P.R.China%Key Laboratory of Chicken Genetics and Breeding,Ministry of Agriculture and Rural Affairs,Harbin 150030,P.R.China</general><general>Key Laboratory of Animal Genetics,Breeding and Reproduction,Heilongjiang General Colleges and Universities,Harbin 150030,P.R.China</general><general>College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,P.R.China</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20220801</creationdate><title>PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene</title><author>SUN, Yu-hang ; ZHAI, Gui-ying ; PANG, Yong-jia ; LI, Rui ; LI, Yu-mao ; CAO, Zhi-ping ; WANG, Ning ; LI, Hui ; WANG, Yu-xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-195692abd883ac4f4812ed75dedf2fc8219d2b1ba2a7bb70b9da68ded522a263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>chicken</topic><topic>isoform</topic><topic>Plin1</topic><topic>PPAR gamma</topic><topic>transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SUN, Yu-hang</creatorcontrib><creatorcontrib>ZHAI, Gui-ying</creatorcontrib><creatorcontrib>PANG, Yong-jia</creatorcontrib><creatorcontrib>LI, Rui</creatorcontrib><creatorcontrib>LI, Yu-mao</creatorcontrib><creatorcontrib>CAO, Zhi-ping</creatorcontrib><creatorcontrib>WANG, Ning</creatorcontrib><creatorcontrib>LI, Hui</creatorcontrib><creatorcontrib>WANG, Yu-xiang</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Integrative Agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SUN, Yu-hang</au><au>ZHAI, Gui-ying</au><au>PANG, Yong-jia</au><au>LI, Rui</au><au>LI, Yu-mao</au><au>CAO, Zhi-ping</au><au>WANG, Ning</au><au>LI, Hui</au><au>WANG, Yu-xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene</atitle><jtitle>Journal of Integrative Agriculture</jtitle><date>2022-08-01</date><risdate>2022</risdate><volume>21</volume><issue>8</issue><spage>2357</spage><epage>2371</epage><pages>2357-2371</pages><issn>2095-3119</issn><eissn>2352-3425</eissn><abstract>Perilipin1 (PLIN1) is a major phosphorylated protein that specifically coats the surface of neutral lipid droplets (LDs) in adipocytes and plays a crucial role in regulating the accumulation and hydrolysis of triacylglycerol (TG). Mammalian studies have shown that Plin1 gene transcription is mainly regulated by peroxisome proliferator-activated receptor-gamma (PPARγ), the master regulator of adipogenesis. However, the regulatory mechanism of the chicken Plin1 (cPlin1) gene is poorly understood. The present study aimed to investigate whether Plin1 is regulated by PPARγ in chickens and identify its exact molecular mechanism. Reporter gene and expression assays showed that PPARγ2, but not PPARγ1, activated (P<0.01) the cPlin1 gene promoter. An electrophoretic mobility shift assay and mutational analysis revealed that PPARγ2 bound to a special site in the cPlin1 gene promoter to enhance its expression. In summary, our results show that PPARγ promotes the expression of the cPlin1 gene and that PPARγ2 is the main regulatory isoform.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S2095-3119(21)63896-0</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | chicken isoform Plin1 PPAR gamma transcriptional regulation |
| title | PPAR gamma2: The main isoform of PPARγ that positively regulates the expression of the chicken Plin1 gene |
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