Immunomodulatory effects of esculetin (6,7-dihydroxycoumarin) on murine lymphocytes and peritoneal macrophages
Coumarins belong to a diverse group of naturally occurring non-nutrient phytochemicals known as benzo-alpha-pyrones. In this study, esculetin, a 6,7-dihydroxy derivative of coumarin with pleiotropic biological activities, was found to have no significant cytotoxic effect on normal murine macrophages...
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| Veröffentlicht in: | Cellular & molecular immunology 2005-06, Vol.2 (3), p.181-188 |
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| creator | Leung, Kwok-nam Leung, Pui-yin Kong, Lai-ping Leung, Po-ki |
| description | Coumarins belong to a diverse group of naturally occurring non-nutrient phytochemicals known as benzo-alpha-pyrones. In this study, esculetin, a 6,7-dihydroxy derivative of coumarin with pleiotropic biological activities, was found to have no significant cytotoxic effect on normal murine macrophages, but it could increase the in vivo migration of the thioglycollate-elicited macrophages in a dose-dependent manner. Moreover, esculetin significantly increased the endocytic activity, and augmented the nitric oxide production and iNOS gene expression in LPS-treated macrophages. In addition, in vivo administration of esculetin into mice was shown to increase the mitogenesis of splenic lymphocytes towards Con A and LPS stimulations, and induced the LAK activity of splenic lymphocytes. Collectively, our results indicate that esculetin could exert immunomodulatory effects on murine macrophages and lymphocytes, both in vitro and in vivo, and this might be one of the possible mechanisms by which coumarins can exert their chemopreventive and anti-tumor activities in vivo. |
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In this study, esculetin, a 6,7-dihydroxy derivative of coumarin with pleiotropic biological activities, was found to have no significant cytotoxic effect on normal murine macrophages, but it could increase the in vivo migration of the thioglycollate-elicited macrophages in a dose-dependent manner. Moreover, esculetin significantly increased the endocytic activity, and augmented the nitric oxide production and iNOS gene expression in LPS-treated macrophages. In addition, in vivo administration of esculetin into mice was shown to increase the mitogenesis of splenic lymphocytes towards Con A and LPS stimulations, and induced the LAK activity of splenic lymphocytes. Collectively, our results indicate that esculetin could exert immunomodulatory effects on murine macrophages and lymphocytes, both in vitro and in vivo, and this might be one of the possible mechanisms by which coumarins can exert their chemopreventive and anti-tumor activities in vivo.</description><identifier>ISSN: 1672-7681</identifier><identifier>PMID: 16212885</identifier><language>eng</language><publisher>China: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China</publisher><subject>Animals ; Cell Movement - drug effects ; Cell Proliferation ; Concanavalin A - pharmacology ; Coumarins - pharmacology ; Endocytosis - drug effects ; Gene Expression Regulation - drug effects ; Killer Cells, Lymphokine-Activated - drug effects ; Killer Cells, Lymphokine-Activated - metabolism ; Lipopolysaccharides - pharmacology ; Lymphocytes - drug effects ; Lymphocytes - immunology ; Macrophages, Peritoneal - drug effects ; Macrophages, Peritoneal - immunology ; Mice ; Mice, Inbred BALB C ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase Type II - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spleen - drug effects ; Spleen - metabolism ; Umbelliferones - chemistry ; Umbelliferones - pharmacology</subject><ispartof>Cellular & molecular immunology, 2005-06, Vol.2 (3), p.181-188</ispartof><rights>Copyright © Wanfang Data Co. 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All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zgmyxzz-e/zgmyxzz-e.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16212885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Leung, Kwok-nam</creatorcontrib><creatorcontrib>Leung, Pui-yin</creatorcontrib><creatorcontrib>Kong, Lai-ping</creatorcontrib><creatorcontrib>Leung, Po-ki</creatorcontrib><title>Immunomodulatory effects of esculetin (6,7-dihydroxycoumarin) on murine lymphocytes and peritoneal macrophages</title><title>Cellular & molecular immunology</title><addtitle>Cell Mol Immunol</addtitle><description>Coumarins belong to a diverse group of naturally occurring non-nutrient phytochemicals known as benzo-alpha-pyrones. In this study, esculetin, a 6,7-dihydroxy derivative of coumarin with pleiotropic biological activities, was found to have no significant cytotoxic effect on normal murine macrophages, but it could increase the in vivo migration of the thioglycollate-elicited macrophages in a dose-dependent manner. Moreover, esculetin significantly increased the endocytic activity, and augmented the nitric oxide production and iNOS gene expression in LPS-treated macrophages. In addition, in vivo administration of esculetin into mice was shown to increase the mitogenesis of splenic lymphocytes towards Con A and LPS stimulations, and induced the LAK activity of splenic lymphocytes. Collectively, our results indicate that esculetin could exert immunomodulatory effects on murine macrophages and lymphocytes, both in vitro and in vivo, and this might be one of the possible mechanisms by which coumarins can exert their chemopreventive and anti-tumor activities in vivo.</description><subject>Animals</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation</subject><subject>Concanavalin A - pharmacology</subject><subject>Coumarins - pharmacology</subject><subject>Endocytosis - drug effects</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Killer Cells, Lymphokine-Activated - drug effects</subject><subject>Killer Cells, Lymphokine-Activated - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Lymphocytes - drug effects</subject><subject>Lymphocytes - immunology</subject><subject>Macrophages, Peritoneal - drug effects</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Spleen - drug effects</subject><subject>Spleen - metabolism</subject><subject>Umbelliferones - chemistry</subject><subject>Umbelliferones - pharmacology</subject><issn>1672-7681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRbMA0VL4BeQVAkSkOHFsZ4kqHpUqsYF15NrjNlVsBzsWpF-PUctmZhbn3pHOWTbHlJU5oxzPsssQ9kVRc8LIRTbDtMQl5_U8sytjonXGqdiL0fkJgdYgx4CcRhBk7GHsLLqjjyxX3W5S3v1M0kUjfGfvkbPIxHQB6icz7JycRghIWIUG8N3oLIgeGSG9G3ZiC-EqO9eiD3B92ovs8-X5Y_mWr99fV8undT6UhIy5bphSkgtJGiKFEFA3iimmC1XWXFU0jaaUG6aILnRRFZxQVjOoCRW4kRWuFtnDsfdbWC3stt276G362B62Zvo5HFook44ULUiCb4_w4N1XhDC2pgsS-l5YcDG0lFOGG_zXenMC48aAagffJQ9T-6-z-gXjTnLC</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Leung, Kwok-nam</creator><creator>Leung, Pui-yin</creator><creator>Kong, Lai-ping</creator><creator>Leung, Po-ki</creator><general>Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20050601</creationdate><title>Immunomodulatory effects of esculetin (6,7-dihydroxycoumarin) on murine lymphocytes and peritoneal macrophages</title><author>Leung, Kwok-nam ; Leung, Pui-yin ; Kong, Lai-ping ; Leung, Po-ki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p244t-f97ddc8ac494caaae59d7d7f0d258d3658d92cb7d4f0f030846757e546a19c313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation</topic><topic>Concanavalin A - pharmacology</topic><topic>Coumarins - pharmacology</topic><topic>Endocytosis - drug effects</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Killer Cells, Lymphokine-Activated - drug effects</topic><topic>Killer Cells, Lymphokine-Activated - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Lymphocytes - drug effects</topic><topic>Lymphocytes - immunology</topic><topic>Macrophages, Peritoneal - drug effects</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase Type II - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Spleen - drug effects</topic><topic>Spleen - metabolism</topic><topic>Umbelliferones - chemistry</topic><topic>Umbelliferones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Leung, Kwok-nam</creatorcontrib><creatorcontrib>Leung, Pui-yin</creatorcontrib><creatorcontrib>Kong, Lai-ping</creatorcontrib><creatorcontrib>Leung, Po-ki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Cellular & molecular immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Leung, Kwok-nam</au><au>Leung, Pui-yin</au><au>Kong, Lai-ping</au><au>Leung, Po-ki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunomodulatory effects of esculetin (6,7-dihydroxycoumarin) on murine lymphocytes and peritoneal macrophages</atitle><jtitle>Cellular & molecular immunology</jtitle><addtitle>Cell Mol Immunol</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>2</volume><issue>3</issue><spage>181</spage><epage>188</epage><pages>181-188</pages><issn>1672-7681</issn><abstract>Coumarins belong to a diverse group of naturally occurring non-nutrient phytochemicals known as benzo-alpha-pyrones. In this study, esculetin, a 6,7-dihydroxy derivative of coumarin with pleiotropic biological activities, was found to have no significant cytotoxic effect on normal murine macrophages, but it could increase the in vivo migration of the thioglycollate-elicited macrophages in a dose-dependent manner. Moreover, esculetin significantly increased the endocytic activity, and augmented the nitric oxide production and iNOS gene expression in LPS-treated macrophages. In addition, in vivo administration of esculetin into mice was shown to increase the mitogenesis of splenic lymphocytes towards Con A and LPS stimulations, and induced the LAK activity of splenic lymphocytes. Collectively, our results indicate that esculetin could exert immunomodulatory effects on murine macrophages and lymphocytes, both in vitro and in vivo, and this might be one of the possible mechanisms by which coumarins can exert their chemopreventive and anti-tumor activities in vivo.</abstract><cop>China</cop><pub>Department of Biochemistry, The Chinese University of Hong Kong, Shatin, Hong Kong, China</pub><pmid>16212885</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Cellular & molecular immunology, 2005-06, Vol.2 (3), p.181-188 |
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| subjects | Animals Cell Movement - drug effects Cell Proliferation Concanavalin A - pharmacology Coumarins - pharmacology Endocytosis - drug effects Gene Expression Regulation - drug effects Killer Cells, Lymphokine-Activated - drug effects Killer Cells, Lymphokine-Activated - metabolism Lipopolysaccharides - pharmacology Lymphocytes - drug effects Lymphocytes - immunology Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - immunology Mice Mice, Inbred BALB C Nitric Oxide - biosynthesis Nitric Oxide Synthase Type II - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Spleen - drug effects Spleen - metabolism Umbelliferones - chemistry Umbelliferones - pharmacology |
| title | Immunomodulatory effects of esculetin (6,7-dihydroxycoumarin) on murine lymphocytes and peritoneal macrophages |
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