Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization
We introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. BMB array has great applicatio...
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| Veröffentlicht in: | Chinese chemical letters 2020-07, Vol.31 (7), p.1812-1816 |
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| creator | Dong, Hongming Tang, Congli He, Ziyu Liu, Hongmei Xu, Yuyue Huang, Hao Yang, Gaojian Xiao, Ziqi Li, Song Deng, Yan Chen, Zhu Chen, Hui Qin, Zuodong Negrin, Yasser Perera He, Nongyue |
| description | We introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. BMB array has great application potential.
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Diarrhea, as a global public health problem, causes a large number of infections and deaths every year. Although Escherichia coli (E. coli) is one of the normal flora microorganisms in the human intestinal tract, it has five pathogenic bacteria types that can cause human diarrhea, known as diarrheagenic E. coli. When people are infected, rapid and accurate diagnosis, along with timely treatment, are especially important. Here, we introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. Results show that the detection sensitivities of enterohemorrhagic E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, enteroinvasive E. coli and enteroaggregative E. coli were 1.3 × 103 CFU/mL, 2 × 104 CFU/mL, 4 × 104 CFU/mL, 7.2 × 104 CFU/mL and 1.7 CFU/mL respectively. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. Compared with other methods, BMB array has great application potential. |
| doi_str_mv | 10.1016/j.cclet.2020.03.002 |
| format | Article |
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[Display omitted]
Diarrhea, as a global public health problem, causes a large number of infections and deaths every year. Although Escherichia coli (E. coli) is one of the normal flora microorganisms in the human intestinal tract, it has five pathogenic bacteria types that can cause human diarrhea, known as diarrheagenic E. coli. When people are infected, rapid and accurate diagnosis, along with timely treatment, are especially important. Here, we introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. Results show that the detection sensitivities of enterohemorrhagic E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, enteroinvasive E. coli and enteroaggregative E. coli were 1.3 × 103 CFU/mL, 2 × 104 CFU/mL, 4 × 104 CFU/mL, 7.2 × 104 CFU/mL and 1.7 CFU/mL respectively. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. Compared with other methods, BMB array has great application potential.</description><identifier>ISSN: 1001-8417</identifier><identifier>EISSN: 1878-5964</identifier><identifier>DOI: 10.1016/j.cclet.2020.03.002</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Barcoded magnetic bead ; Detect multiple target ; Diarrheagenic E. coli ; Multiplex PCR ; Rapid detection</subject><ispartof>Chinese chemical letters, 2020-07, Vol.31 (7), p.1812-1816</ispartof><rights>2020 The Author</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-4ac148151bdb302acb5fb5fca156b2f4105ee9920ab3713134d236e469f186123</citedby><cites>FETCH-LOGICAL-c335t-4ac148151bdb302acb5fb5fca156b2f4105ee9920ab3713134d236e469f186123</cites><orcidid>0000-0002-5826-5752 ; 0000-0002-1246-3390</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zghxkb/zghxkb.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cclet.2020.03.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Dong, Hongming</creatorcontrib><creatorcontrib>Tang, Congli</creatorcontrib><creatorcontrib>He, Ziyu</creatorcontrib><creatorcontrib>Liu, Hongmei</creatorcontrib><creatorcontrib>Xu, Yuyue</creatorcontrib><creatorcontrib>Huang, Hao</creatorcontrib><creatorcontrib>Yang, Gaojian</creatorcontrib><creatorcontrib>Xiao, Ziqi</creatorcontrib><creatorcontrib>Li, Song</creatorcontrib><creatorcontrib>Deng, Yan</creatorcontrib><creatorcontrib>Chen, Zhu</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Qin, Zuodong</creatorcontrib><creatorcontrib>Negrin, Yasser Perera</creatorcontrib><creatorcontrib>He, Nongyue</creatorcontrib><title>Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization</title><title>Chinese chemical letters</title><description>We introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. BMB array has great application potential.
[Display omitted]
Diarrhea, as a global public health problem, causes a large number of infections and deaths every year. Although Escherichia coli (E. coli) is one of the normal flora microorganisms in the human intestinal tract, it has five pathogenic bacteria types that can cause human diarrhea, known as diarrheagenic E. coli. When people are infected, rapid and accurate diagnosis, along with timely treatment, are especially important. Here, we introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. Results show that the detection sensitivities of enterohemorrhagic E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, enteroinvasive E. coli and enteroaggregative E. coli were 1.3 × 103 CFU/mL, 2 × 104 CFU/mL, 4 × 104 CFU/mL, 7.2 × 104 CFU/mL and 1.7 CFU/mL respectively. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. Compared with other methods, BMB array has great application potential.</description><subject>Barcoded magnetic bead</subject><subject>Detect multiple target</subject><subject>Diarrheagenic E. coli</subject><subject>Multiplex PCR</subject><subject>Rapid detection</subject><issn>1001-8417</issn><issn>1878-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kE1PwzAMhisEEmPwC7j0xqklafq1Awc0jQ9pEhKCc-QkTuuytVNSPrZfT7ZxRrJkH57Xlp8ouuYs5YyXt12q9QrHNGMZS5lIGctOogmvqzopZmV-GmbGeFLnvDqPLrzvAlDXopxE5hU2ZGIy2I9kScNIQx8PNjYEzrUIDfak44XXLTrSLUGshxXFCjyaOKAKnB5MmNfQ9DgGViGYuN0qR4Z2h32X0ZmFlcervz6N3h8Wb_OnZPny-Dy_XyZaiGJMctA8r3nBlVGCZaBVYUNp4EWpMptzViDOZhkDJSouuMhNJkrMy5nldckzMY1ujnu_obfQN7IbPl0fLspd0_58qL0eVgVhgRRHUrvBe4dWbhytwW0lZ3KvVHbyoFTuM5IJGYyF1N0xheGJL0InvSbsNRpyqEdpBvo3_wvSMYEJ</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Dong, Hongming</creator><creator>Tang, Congli</creator><creator>He, Ziyu</creator><creator>Liu, Hongmei</creator><creator>Xu, Yuyue</creator><creator>Huang, Hao</creator><creator>Yang, Gaojian</creator><creator>Xiao, Ziqi</creator><creator>Li, Song</creator><creator>Deng, Yan</creator><creator>Chen, Zhu</creator><creator>Chen, Hui</creator><creator>Qin, Zuodong</creator><creator>Negrin, Yasser Perera</creator><creator>He, Nongyue</creator><general>Elsevier B.V</general><general>Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China%Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China</general><general>State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China%Hunan Engineering Technology Research Center for Comprehensive Development and Utilization of Biomass Resources, Hunan University of Science and Engineering, Yongzhou 425199, China%China-Cuba Biotechnology Joint Innovation Center (CCBJIC), Yongzhou Zhong Gu Biotechnology Co., Yongzhou 425000, China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><orcidid>https://orcid.org/0000-0002-5826-5752</orcidid><orcidid>https://orcid.org/0000-0002-1246-3390</orcidid></search><sort><creationdate>20200701</creationdate><title>Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization</title><author>Dong, Hongming ; Tang, Congli ; He, Ziyu ; Liu, Hongmei ; Xu, Yuyue ; Huang, Hao ; Yang, Gaojian ; Xiao, Ziqi ; Li, Song ; Deng, Yan ; Chen, Zhu ; Chen, Hui ; Qin, Zuodong ; Negrin, Yasser Perera ; He, Nongyue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-4ac148151bdb302acb5fb5fca156b2f4105ee9920ab3713134d236e469f186123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Barcoded magnetic bead</topic><topic>Detect multiple target</topic><topic>Diarrheagenic E. coli</topic><topic>Multiplex PCR</topic><topic>Rapid detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Hongming</creatorcontrib><creatorcontrib>Tang, Congli</creatorcontrib><creatorcontrib>He, Ziyu</creatorcontrib><creatorcontrib>Liu, Hongmei</creatorcontrib><creatorcontrib>Xu, Yuyue</creatorcontrib><creatorcontrib>Huang, Hao</creatorcontrib><creatorcontrib>Yang, Gaojian</creatorcontrib><creatorcontrib>Xiao, Ziqi</creatorcontrib><creatorcontrib>Li, Song</creatorcontrib><creatorcontrib>Deng, Yan</creatorcontrib><creatorcontrib>Chen, Zhu</creatorcontrib><creatorcontrib>Chen, Hui</creatorcontrib><creatorcontrib>Qin, Zuodong</creatorcontrib><creatorcontrib>Negrin, Yasser Perera</creatorcontrib><creatorcontrib>He, Nongyue</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese chemical letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Hongming</au><au>Tang, Congli</au><au>He, Ziyu</au><au>Liu, Hongmei</au><au>Xu, Yuyue</au><au>Huang, Hao</au><au>Yang, Gaojian</au><au>Xiao, Ziqi</au><au>Li, Song</au><au>Deng, Yan</au><au>Chen, Zhu</au><au>Chen, Hui</au><au>Qin, Zuodong</au><au>Negrin, Yasser Perera</au><au>He, Nongyue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization</atitle><jtitle>Chinese chemical letters</jtitle><date>2020-07-01</date><risdate>2020</risdate><volume>31</volume><issue>7</issue><spage>1812</spage><epage>1816</epage><pages>1812-1816</pages><issn>1001-8417</issn><eissn>1878-5964</eissn><abstract>We introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. BMB array has great application potential.
[Display omitted]
Diarrhea, as a global public health problem, causes a large number of infections and deaths every year. Although Escherichia coli (E. coli) is one of the normal flora microorganisms in the human intestinal tract, it has five pathogenic bacteria types that can cause human diarrhea, known as diarrheagenic E. coli. When people are infected, rapid and accurate diagnosis, along with timely treatment, are especially important. Here, we introduce a new method to identify and analyze a large number of pathogenic strains in E. coli by multiplex PCR and barcoded magnetic bead hybridization. Results show that the detection sensitivities of enterohemorrhagic E. coli, enterotoxigenic E. coli, enteropathogenic E. coli, enteroinvasive E. coli and enteroaggregative E. coli were 1.3 × 103 CFU/mL, 2 × 104 CFU/mL, 4 × 104 CFU/mL, 7.2 × 104 CFU/mL and 1.7 CFU/mL respectively. This method has strong specificity and high sensitivity and detects multiple target sequences in one experiment. Compared with other methods, BMB array has great application potential.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.cclet.2020.03.002</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-5826-5752</orcidid><orcidid>https://orcid.org/0000-0002-1246-3390</orcidid></addata></record> |
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| subjects | Barcoded magnetic bead Detect multiple target Diarrheagenic E. coli Multiplex PCR Rapid detection |
| title | Rapid identification of diarrheagenic Escherichia coli based on barcoded magnetic bead hybridization |
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