Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon
Detection for deoxyribozyme （DNAzyme） cleavage usually needs complex and time-consuming radial labeling, gel electro- phoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary...
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| Veröffentlicht in: | Chinese chemical letters 2012-10, Vol.23 (10), p.1177-1180 |
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| container_title | Chinese chemical letters |
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| creator | Xie, Hong Peng Meng, Xiang Xian Su, Hongyan Cai, Qing Yun Tan, Yong Jun Huang, Xiao Qin |
| description | Detection for deoxyribozyme （DNAzyme） cleavage usually needs complex and time-consuming radial labeling, gel electro- phoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy. |
| doi_str_mv | 10.1016/j.cclet.2012.07.015 |
| format | Article |
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A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. 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A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy.</description><subject>DNAzyme cleavage</subject><subject>HCV-RNA</subject><subject>Ligation</subject><subject>Molecular beacon</subject><subject>凝胶电泳</subject><subject>分子信标</subject><subject>切割</subject><subject>放射自显影</subject><subject>脱氧核酶</subject><subject>裂解产物</subject><subject>超灵敏检测</subject><subject>连接酶</subject><issn>1001-8417</issn><issn>1878-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkDtPwzAUhSMEEs9fwGImpgQ7TmJnYKh4SxUsILFZjn0TXFK72Emh_HpcWrGy3HuHc-7R-ZLklOCMYFJdzDKlehiyHJM8wyzDpNxJDghnPC3rqtiNN8Yk5QVh-8lhCDOMc85pdZC8Tk0nA6RNHBqN_eDjYYMZzBKQhgHUYJxFrkXXj5Pv1RxQDJJL2QFaeKdHNaAxGNuhuetBjb30qAGpnD1O9lrZBzjZ7qPk5fbm-eo-nT7dPVxNpqmiFRlSVeOClpwxrpnURZErXuOWaKlqpXBdlJoz3EKes5KTlkBVtk1TRS3lJa8Zo0fJ-ebvp7SttJ2YudHbmCi-u7ev92aNJJbPcVTSjVJ5F4KHViy8mUu_EgSLNUYxE78YxdojMBMRY3RdblwQSywNeBGUAatAGx_hCO3MP_6zbeqbs91HRPUXW1BGeUVz-gONK4gF</recordid><startdate>20121001</startdate><enddate>20121001</enddate><creator>Xie, Hong Peng</creator><creator>Meng, Xiang Xian</creator><creator>Su, Hongyan</creator><creator>Cai, Qing Yun</creator><creator>Tan, Yong Jun</creator><creator>Huang, Xiao Qin</creator><general>Elsevier B.V</general><general>School of Biology, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20121001</creationdate><title>Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon</title><author>Xie, Hong Peng ; Meng, Xiang Xian ; Su, Hongyan ; Cai, Qing Yun ; Tan, Yong Jun ; Huang, Xiao Qin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-c904358778d7ad442c890f1dac9cc0945d870fe227581f1e65fbb6d7a38589773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>DNAzyme cleavage</topic><topic>HCV-RNA</topic><topic>Ligation</topic><topic>Molecular beacon</topic><topic>凝胶电泳</topic><topic>分子信标</topic><topic>切割</topic><topic>放射自显影</topic><topic>脱氧核酶</topic><topic>裂解产物</topic><topic>超灵敏检测</topic><topic>连接酶</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Hong Peng</creatorcontrib><creatorcontrib>Meng, Xiang Xian</creatorcontrib><creatorcontrib>Su, Hongyan</creatorcontrib><creatorcontrib>Cai, Qing Yun</creatorcontrib><creatorcontrib>Tan, Yong Jun</creatorcontrib><creatorcontrib>Huang, Xiao Qin</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese chemical letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Hong Peng</au><au>Meng, Xiang Xian</au><au>Su, Hongyan</au><au>Cai, Qing Yun</au><au>Tan, Yong Jun</au><au>Huang, Xiao Qin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon</atitle><jtitle>Chinese chemical letters</jtitle><addtitle>Chinese Chemical Letters</addtitle><date>2012-10-01</date><risdate>2012</risdate><volume>23</volume><issue>10</issue><spage>1177</spage><epage>1180</epage><pages>1177-1180</pages><issn>1001-8417</issn><eissn>1878-5964</eissn><abstract>Detection for deoxyribozyme （DNAzyme） cleavage usually needs complex and time-consuming radial labeling, gel electro- phoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.cclet.2012.07.015</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1001-8417 |
| ispartof | Chinese chemical letters, 2012-10, Vol.23 (10), p.1177-1180 |
| issn | 1001-8417 1878-5964 |
| language | eng |
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| source | Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | DNAzyme cleavage HCV-RNA Ligation Molecular beacon 凝胶电泳 分子信标 切割 放射自显影 脱氧核酶 裂解产物 超灵敏检测 连接酶 |
| title | Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon |
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