Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier
N-Methylene phosphonic chitosan (NMPCS), an amphiphilic macromolecule with powerful chelating ability of Ca 2+ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR, 13C NMR, X-ray, agarose gel el...
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| Veröffentlicht in: | Chinese chemical letters 2007-11, Vol.18 (11), p.1407-1410 |
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| container_title | Chinese chemical letters |
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| creator | Zhu, Dun Wan Bo, Jin Gen Zhang, Hai Ling Liu, Wen Guang Leng, Xi Gang Song, Cun Xian Yin, Yu Ji Song, Li Ping Liu, Lan Xia Mei, Lin Li, Xiu Lan Zhang, Yang De Yao, Kang |
| description | N-Methylene phosphonic chitosan (NMPCS), an amphiphilic macromolecule with powerful chelating ability of Ca
2+ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,
13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy (AFM) and circular dichroism (CD). The results suggest that at charge ratio 2:1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80–210
nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan. |
| doi_str_mv | 10.1016/j.cclet.2007.09.006 |
| format | Article |
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2+ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,
13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy (AFM) and circular dichroism (CD). The results suggest that at charge ratio 2:1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80–210
nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.</description><identifier>ISSN: 1001-8417</identifier><identifier>EISSN: 1878-5964</identifier><identifier>DOI: 10.1016/j.cclet.2007.09.006</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Gene delivery ; N-Methylene phosphonic chitosan ; Non-viral vector</subject><ispartof>Chinese chemical letters, 2007-11, Vol.18 (11), p.1407-1410</ispartof><rights>2007 Kang De Yao</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-eaf0851ab43e578508099cbee4eb62fa8749f11410843af64fef85a6b59811f83</citedby><cites>FETCH-LOGICAL-c335t-eaf0851ab43e578508099cbee4eb62fa8749f11410843af64fef85a6b59811f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zghxkb/zghxkb.jpg</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1001841707003804$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids></links><search><creatorcontrib>Zhu, Dun Wan</creatorcontrib><creatorcontrib>Bo, Jin Gen</creatorcontrib><creatorcontrib>Zhang, Hai Ling</creatorcontrib><creatorcontrib>Liu, Wen Guang</creatorcontrib><creatorcontrib>Leng, Xi Gang</creatorcontrib><creatorcontrib>Song, Cun Xian</creatorcontrib><creatorcontrib>Yin, Yu Ji</creatorcontrib><creatorcontrib>Song, Li Ping</creatorcontrib><creatorcontrib>Liu, Lan Xia</creatorcontrib><creatorcontrib>Mei, Lin</creatorcontrib><creatorcontrib>Li, Xiu Lan</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>De Yao, Kang</creatorcontrib><title>Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier</title><title>Chinese chemical letters</title><description>N-Methylene phosphonic chitosan (NMPCS), an amphiphilic macromolecule with powerful chelating ability of Ca
2+ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,
13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy (AFM) and circular dichroism (CD). The results suggest that at charge ratio 2:1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80–210
nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.</description><subject>Gene delivery</subject><subject>N-Methylene phosphonic chitosan</subject><subject>Non-viral vector</subject><issn>1001-8417</issn><issn>1878-5964</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhi0EEqXwC1i8AUPCOZ_OwIAqvqRSkAqsluOeG4fiVLb5KL-ehDIznO6G97nTPYQcM4gZsOK8jZVaYYgTgDKGKgYodsiI8ZJHeVVku_0MwCKesXKfHHjfAiScp8WIvMw3NjTojaedprPoDUOzWaFFum4635c1iqrGhM5LS09n94-T-RmVdkFN8HTdBbTByBWVni4HSknnDLpDsqflyuPRXx-T5-urp8ltNH24uZtcTiOVpnmIUGrgOZN1lmJe8hw4VJWqETOsi0RLXmaVZixjwLNU6iLTqHkuizqvOGOap2Nyst37Ka2Wdina7t3Z_qL4XjZfr_UghDFIqj6ZbpPKdd471GLtzJt0G8FADBJFK34lioERUIleYk9dbCnsn_joHxNeGbQKF8ahCmLRmX_5H-yEe1c</recordid><startdate>20071101</startdate><enddate>20071101</enddate><creator>Zhu, Dun Wan</creator><creator>Bo, Jin Gen</creator><creator>Zhang, Hai Ling</creator><creator>Liu, Wen Guang</creator><creator>Leng, Xi Gang</creator><creator>Song, Cun Xian</creator><creator>Yin, Yu Ji</creator><creator>Song, Li Ping</creator><creator>Liu, Lan Xia</creator><creator>Mei, Lin</creator><creator>Li, Xiu Lan</creator><creator>Zhang, Yang</creator><creator>De Yao, Kang</creator><general>Elsevier B.V</general><general>Research Institute of Polymeric Materials, Tianjin University, Tianjin 300072, China%Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering,Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China%Institute of Orthopedics, Tianjin Hospital, Tianjin 300211, China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20071101</creationdate><title>Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier</title><author>Zhu, Dun Wan ; Bo, Jin Gen ; Zhang, Hai Ling ; Liu, Wen Guang ; Leng, Xi Gang ; Song, Cun Xian ; Yin, Yu Ji ; Song, Li Ping ; Liu, Lan Xia ; Mei, Lin ; Li, Xiu Lan ; Zhang, Yang ; De Yao, Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-eaf0851ab43e578508099cbee4eb62fa8749f11410843af64fef85a6b59811f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Gene delivery</topic><topic>N-Methylene phosphonic chitosan</topic><topic>Non-viral vector</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Dun Wan</creatorcontrib><creatorcontrib>Bo, Jin Gen</creatorcontrib><creatorcontrib>Zhang, Hai Ling</creatorcontrib><creatorcontrib>Liu, Wen Guang</creatorcontrib><creatorcontrib>Leng, Xi Gang</creatorcontrib><creatorcontrib>Song, Cun Xian</creatorcontrib><creatorcontrib>Yin, Yu Ji</creatorcontrib><creatorcontrib>Song, Li Ping</creatorcontrib><creatorcontrib>Liu, Lan Xia</creatorcontrib><creatorcontrib>Mei, Lin</creatorcontrib><creatorcontrib>Li, Xiu Lan</creatorcontrib><creatorcontrib>Zhang, Yang</creatorcontrib><creatorcontrib>De Yao, Kang</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese chemical letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Dun Wan</au><au>Bo, Jin Gen</au><au>Zhang, Hai Ling</au><au>Liu, Wen Guang</au><au>Leng, Xi Gang</au><au>Song, Cun Xian</au><au>Yin, Yu Ji</au><au>Song, Li Ping</au><au>Liu, Lan Xia</au><au>Mei, Lin</au><au>Li, Xiu Lan</au><au>Zhang, Yang</au><au>De Yao, Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier</atitle><jtitle>Chinese chemical letters</jtitle><date>2007-11-01</date><risdate>2007</risdate><volume>18</volume><issue>11</issue><spage>1407</spage><epage>1410</epage><pages>1407-1410</pages><issn>1001-8417</issn><eissn>1878-5964</eissn><abstract>N-Methylene phosphonic chitosan (NMPCS), an amphiphilic macromolecule with powerful chelating ability of Ca
2+ ions, was synthesized and characterized. The physicochemical properties of NMPCS and the interactions between NMPCS and plasmid DNA were investigated by FTIR,
13C NMR, X-ray, agarose gel electrophoresis retardation assay, atomic force microscopy (AFM) and circular dichroism (CD). The results suggest that at charge ratio 2:1 or above, DNA could be completely entrapped and spherical complexes with mean size of 80–210
nm were formed. Taking HeLa as host cell, luciferase expression mediated by NMPCS improved about 100 times compared to the expression mediated by chitosan.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.cclet.2007.09.006</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1001-8417 |
| ispartof | Chinese chemical letters, 2007-11, Vol.18 (11), p.1407-1410 |
| issn | 1001-8417 1878-5964 |
| language | eng |
| recordid | cdi_wanfang_journals_zghxkb200711029 |
| source | Elsevier ScienceDirect Journals; Alma/SFX Local Collection |
| subjects | Gene delivery N-Methylene phosphonic chitosan Non-viral vector |
| title | Synthesis of N-methylene phosphonic chitosan (NMPCS) and its potential as gene carrier |
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