Mechanism of Drug Resistance Identified in Human Lung Adenocarcinoma Cell Line SPC-A1 Selected for Resistance to Docetaxel

Objective： To investigate the mechanism of resistance to docetaxel in human lung cancer. Methods： Human lung carcinoma SPC-A1/Docetaxel cells were derived from parental SPC-A1 cells by continuous exposure to increasing concentration of docetaxel. The drug sensitivity was measured by MTT assay in vit...

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Veröffentlicht in:	Chinese journal of cancer research 2009-09, Vol.21 (3), p.207-216
Hauptverfasser:	Sun, Hai, Chen, Long-bang
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Cancer Pharmacology cDNA微阵列 Medicine Medicine & Public Health SPC 多西紫杉醇差异表达基因流式细胞仪分析耐药性耐药机制肺癌细胞
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container_title	Chinese journal of cancer research
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creator	Sun, Hai Chen, Long-bang
description	Objective： To investigate the mechanism of resistance to docetaxel in human lung cancer. Methods： Human lung carcinoma SPC-A1/Docetaxel cells were derived from parental SPC-A1 cells by continuous exposure to increasing concentration of docetaxel. The drug sensitivity was measured by MTT assay in vitro. The cDNA microarray identified a set of differentially expressed genes, and some genes were confirmed by RT-PCR. P-glycoprotein level was measured by flow cytometry analysis. Results： The results of drug sensitivity measured by MTT assay showed that SPC-A1/Docetaxel cells were 13.2-fold resistant to docetaxel and cross-resistant at varying levels to other drugs. The cDNA microarray results identified a set of differentially expressed genes, which showed 428 genes that were up-regulated and 506 genes that were down-regulated in SPC-A1/Docetaxel ceils, and some genes were confirmed by RT-PCR. Flow cytometry analysis suggests expression of P-glycoprotein （P-gp） was more abundant in SPC-A1/Docetaxel cells than in the parental cells and docetaxel selection reduces the apoptotic response. Conclusion： The results suggest that docetaxel selection led to changes in gene expression that contribute to the multidrug resistance phenotype.
doi_str_mv	10.1007/s11670-009-0207-4
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Methods： Human lung carcinoma SPC-A1/Docetaxel cells were derived from parental SPC-A1 cells by continuous exposure to increasing concentration of docetaxel. The drug sensitivity was measured by MTT assay in vitro. The cDNA microarray identified a set of differentially expressed genes, and some genes were confirmed by RT-PCR. P-glycoprotein level was measured by flow cytometry analysis. Results： The results of drug sensitivity measured by MTT assay showed that SPC-A1/Docetaxel cells were 13.2-fold resistant to docetaxel and cross-resistant at varying levels to other drugs. The cDNA microarray results identified a set of differentially expressed genes, which showed 428 genes that were up-regulated and 506 genes that were down-regulated in SPC-A1/Docetaxel ceils, and some genes were confirmed by RT-PCR. Flow cytometry analysis suggests expression of P-glycoprotein （P-gp） was more abundant in SPC-A1/Docetaxel cells than in the parental cells and docetaxel selection reduces the apoptotic response. Conclusion： The results suggest that docetaxel selection led to changes in gene expression that contribute to the multidrug resistance phenotype.</description><identifier>ISSN: 1000-9604</identifier><identifier>EISSN: 1993-0631</identifier><identifier>DOI: 10.1007/s11670-009-0207-4</identifier><language>eng</language><publisher>Heidelberg: Chinese Anti-Cancer Association</publisher><subject>Anti-Cancer Pharmacology ; cDNA微阵列 ; Medicine ; Medicine & Public Health ; SPC ; 多西紫杉醇 ; 差异表达基因 ; 流式细胞仪分析 ; 耐药性 ; 耐药机制 ; 肺癌细胞</subject><ispartof>Chinese journal of cancer research, 2009-09, Vol.21 (3), p.207-216</ispartof><rights>Chinese Anti-Cancer Association and Springer-Verlag GmbH 2009</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-12ccb8700d099eeccbd2da588b5df88bedb78d1854825bf72b91bf56f6e01f4b3</citedby><cites>FETCH-LOGICAL-c347t-12ccb8700d099eeccbd2da588b5df88bedb78d1854825bf72b91bf56f6e01f4b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85190X/85190X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Sun, Hai</creatorcontrib><creatorcontrib>Chen, Long-bang</creatorcontrib><title>Mechanism of Drug Resistance Identified in Human Lung Adenocarcinoma Cell Line SPC-A1 Selected for Resistance to Docetaxel</title><title>Chinese journal of cancer research</title><addtitle>Chin. J. Cancer Res</addtitle><addtitle>Chinese Journal of Cancer Research</addtitle><description>Objective： To investigate the mechanism of resistance to docetaxel in human lung cancer. Methods： Human lung carcinoma SPC-A1/Docetaxel cells were derived from parental SPC-A1 cells by continuous exposure to increasing concentration of docetaxel. The drug sensitivity was measured by MTT assay in vitro. The cDNA microarray identified a set of differentially expressed genes, and some genes were confirmed by RT-PCR. P-glycoprotein level was measured by flow cytometry analysis. Results： The results of drug sensitivity measured by MTT assay showed that SPC-A1/Docetaxel cells were 13.2-fold resistant to docetaxel and cross-resistant at varying levels to other drugs. The cDNA microarray results identified a set of differentially expressed genes, which showed 428 genes that were up-regulated and 506 genes that were down-regulated in SPC-A1/Docetaxel ceils, and some genes were confirmed by RT-PCR. Flow cytometry analysis suggests expression of P-glycoprotein （P-gp） was more abundant in SPC-A1/Docetaxel cells than in the parental cells and docetaxel selection reduces the apoptotic response. Conclusion： The results suggest that docetaxel selection led to changes in gene expression that contribute to the multidrug resistance phenotype.</description><subject>Anti-Cancer Pharmacology</subject><subject>cDNA微阵列</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>SPC</subject><subject>多西紫杉醇</subject><subject>差异表达基因</subject><subject>流式细胞仪分析</subject><subject>耐药性</subject><subject>耐药机制</subject><subject>肺癌细胞</subject><issn>1000-9604</issn><issn>1993-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLJDEURgtxwMf4A9wFN67i3Jt6L5v2CS0zjLoOqdRNmbY60aQax_71RloYV26SXPKd78LJsmOEMwSof0XEqgYO0HIQUPNiJ9vHts05VDnupjcA8LaCYi87iHEJUNYl4H62uSX9qJyNK-YNOw_rgf2laOOknCZ205ObrLHUM-vY9XqlHFus3cBm6cNrFbR1fqXYnMaRLawjdvdnzmfI7mgkPSXM-PC1cPLs3Gua1D8af2Y_jBojHX3eh9nD5cX9_Jovfl_dzGcLrvOinjgKrbumBuihbYnS0ItelU3Tlb1JJ_Vd3fTYlEUjys7UomuxM2VlKgI0RZcfZqfb3lfljHKDXPp1cGmj3Axq87YUSRrkSWJK4japg48xkJHPwa5UeJMI8kOz3GqWiZAfmmWRGLFlYsq6gcL_-u-gk89Fj94NL4mTndJPxo4kcxRYCMT8HTAui9Y</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Sun, Hai</creator><creator>Chen, Long-bang</creator><general>Chinese Anti-Cancer Association</general><general>Department of Medical Oncology, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20090901</creationdate><title>Mechanism of Drug Resistance Identified in Human Lung Adenocarcinoma Cell Line SPC-A1 Selected for Resistance to Docetaxel</title><author>Sun, Hai ; Chen, Long-bang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-12ccb8700d099eeccbd2da588b5df88bedb78d1854825bf72b91bf56f6e01f4b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anti-Cancer Pharmacology</topic><topic>cDNA微阵列</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>SPC</topic><topic>多西紫杉醇</topic><topic>差异表达基因</topic><topic>流式细胞仪分析</topic><topic>耐药性</topic><topic>耐药机制</topic><topic>肺癌细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Hai</creatorcontrib><creatorcontrib>Chen, Long-bang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese journal of cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Hai</au><au>Chen, Long-bang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of Drug Resistance Identified in Human Lung Adenocarcinoma Cell Line SPC-A1 Selected for Resistance to Docetaxel</atitle><jtitle>Chinese journal of cancer research</jtitle><stitle>Chin. J. Cancer Res</stitle><addtitle>Chinese Journal of Cancer Research</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>21</volume><issue>3</issue><spage>207</spage><epage>216</epage><pages>207-216</pages><issn>1000-9604</issn><eissn>1993-0631</eissn><abstract>Objective： To investigate the mechanism of resistance to docetaxel in human lung cancer. Methods： Human lung carcinoma SPC-A1/Docetaxel cells were derived from parental SPC-A1 cells by continuous exposure to increasing concentration of docetaxel. The drug sensitivity was measured by MTT assay in vitro. The cDNA microarray identified a set of differentially expressed genes, and some genes were confirmed by RT-PCR. P-glycoprotein level was measured by flow cytometry analysis. Results： The results of drug sensitivity measured by MTT assay showed that SPC-A1/Docetaxel cells were 13.2-fold resistant to docetaxel and cross-resistant at varying levels to other drugs. The cDNA microarray results identified a set of differentially expressed genes, which showed 428 genes that were up-regulated and 506 genes that were down-regulated in SPC-A1/Docetaxel ceils, and some genes were confirmed by RT-PCR. Flow cytometry analysis suggests expression of P-glycoprotein （P-gp） was more abundant in SPC-A1/Docetaxel cells than in the parental cells and docetaxel selection reduces the apoptotic response. Conclusion： The results suggest that docetaxel selection led to changes in gene expression that contribute to the multidrug resistance phenotype.</abstract><cop>Heidelberg</cop><pub>Chinese Anti-Cancer Association</pub><doi>10.1007/s11670-009-0207-4</doi><tpages>10</tpages></addata></record>
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source	EZB-FREE-00999 freely available EZB journals
subjects	Anti-Cancer Pharmacology cDNA微阵列 Medicine Medicine & Public Health SPC 多西紫杉醇差异表达基因流式细胞仪分析耐药性耐药机制肺癌细胞
title	Mechanism of Drug Resistance Identified in Human Lung Adenocarcinoma Cell Line SPC-A1 Selected for Resistance to Docetaxel
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