Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells
Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： Accordi...
Gespeichert in:
| Veröffentlicht in: | Chinese journal of cancer research 2008-03, Vol.20 (1), p.1-4 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4 |
|---|---|
| container_issue | 1 |
| container_start_page | 1 |
| container_title | Chinese journal of cancer research |
| container_volume | 20 |
| creator | 陈玮 赵涌 卞修武 |
| description | Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins. |
| doi_str_mv | 10.1007/s11670-008-0001-8 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_cross</sourceid><recordid>TN_cdi_wanfang_journals_zgazyj200801001</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>26680611</cqvip_id><wanfj_id>zgazyj200801001</wanfj_id><sourcerecordid>zgazyj200801001</sourcerecordid><originalsourceid>FETCH-LOGICAL-c347t-69728975773bfae1c79bf79c0cbf6fdc08b5b8ffa7e42e80960f03407b07a71a3</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhiMEEqXwA9gsFqbAOR92MqIqtJUqYCislu3YaUpig92C0l-PUSqxMZzudHqf-3ij6BrDHQag9x5jQiEGKEIAjouTaILLMo2BpPg01KEblwSy8-jC-y1ATnPAk2i9NJtWtDvrBlRpreQOWY2erKsHZA1avLxhgiqC5soo1IbGvucGLfveuh3v2oOqUWVqK5X7aiXv0Ex1nb-MzjTvvLo65mn0-litZ4t49Txfzh5WsUwzuotJSZOipDmlqdBcYUlLoWkpQQpNdC2hELkotOZUZYkqIJyvIc2ACqCcYp5Oo9tx7jc3mpuGbe3embCRHRp-GLZJsAPC5zgo8aiUznrvlGYfru25GxgG9msgGw1kgWC_BrIiMMnI-KA1jXJ_4_-Dbo6LNtY0n4Fjgst33XaKJYQUQDBOfwALPHzp</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells</title><source>EZB Electronic Journals Library</source><creator>陈玮 赵涌 卞修武</creator><creatorcontrib>陈玮 赵涌 卞修武</creatorcontrib><description>Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.</description><identifier>ISSN: 1000-9604</identifier><identifier>EISSN: 1993-0631</identifier><identifier>DOI: 10.1007/s11670-008-0001-8</identifier><language>eng</language><publisher>Heidelberg: Chinese Anti-Cancer Association</publisher><subject>Basic Research ; Medicine ; Medicine & Public Health ; 人乳头状瘤病毒 ; 细胞增殖 ; 蛋白质</subject><ispartof>Chinese journal of cancer research, 2008-03, Vol.20 (1), p.1-4</ispartof><rights>Chinese Anti-Cancer Association 2008</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-69728975773bfae1c79bf79c0cbf6fdc08b5b8ffa7e42e80960f03407b07a71a3</citedby><cites>FETCH-LOGICAL-c347t-69728975773bfae1c79bf79c0cbf6fdc08b5b8ffa7e42e80960f03407b07a71a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85190X/85190X.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>陈玮 赵涌 卞修武</creatorcontrib><title>Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells</title><title>Chinese journal of cancer research</title><addtitle>Chin. J. Cancer Res</addtitle><addtitle>Chinese Journal of Cancer Research</addtitle><description>Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.</description><subject>Basic Research</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>人乳头状瘤病毒</subject><subject>细胞增殖</subject><subject>蛋白质</subject><issn>1000-9604</issn><issn>1993-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAQhiMEEqXwA9gsFqbAOR92MqIqtJUqYCislu3YaUpig92C0l-PUSqxMZzudHqf-3ij6BrDHQag9x5jQiEGKEIAjouTaILLMo2BpPg01KEblwSy8-jC-y1ATnPAk2i9NJtWtDvrBlRpreQOWY2erKsHZA1avLxhgiqC5soo1IbGvucGLfveuh3v2oOqUWVqK5X7aiXv0Ex1nb-MzjTvvLo65mn0-litZ4t49Txfzh5WsUwzuotJSZOipDmlqdBcYUlLoWkpQQpNdC2hELkotOZUZYkqIJyvIc2ACqCcYp5Oo9tx7jc3mpuGbe3embCRHRp-GLZJsAPC5zgo8aiUznrvlGYfru25GxgG9msgGw1kgWC_BrIiMMnI-KA1jXJ_4_-Dbo6LNtY0n4Fjgst33XaKJYQUQDBOfwALPHzp</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>陈玮 赵涌 卞修武</creator><general>Chinese Anti-Cancer Association</general><general>Department of Pathology,Chongqing University of Medical Sciences,Chongqing 400016%Institute of Pathology,Southwest Hospital,Third Military Medical University,Chongqing 400038</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20080301</creationdate><title>Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells</title><author>陈玮 赵涌 卞修武</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-69728975773bfae1c79bf79c0cbf6fdc08b5b8ffa7e42e80960f03407b07a71a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Basic Research</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>人乳头状瘤病毒</topic><topic>细胞增殖</topic><topic>蛋白质</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>陈玮 赵涌 卞修武</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Chinese journal of cancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>陈玮 赵涌 卞修武</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells</atitle><jtitle>Chinese journal of cancer research</jtitle><stitle>Chin. J. Cancer Res</stitle><addtitle>Chinese Journal of Cancer Research</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>20</volume><issue>1</issue><spage>1</spage><epage>4</epage><pages>1-4</pages><issn>1000-9604</issn><eissn>1993-0631</eissn><abstract>Objective： To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells （H8）. Methods： The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry, and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results： According to immunocytochemistry, expressions of E6 and p53 proteins of H8 cells after treatment with 100 μtmol/L Nordy for 72 h were weaker than control group obviously. After treatment with 50, 100, 200 μmol/L Nordy for 72 h. HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly （P〈0.05）. Conclusion： Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells, influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.</abstract><cop>Heidelberg</cop><pub>Chinese Anti-Cancer Association</pub><doi>10.1007/s11670-008-0001-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1000-9604 |
| ispartof | Chinese journal of cancer research, 2008-03, Vol.20 (1), p.1-4 |
| issn | 1000-9604 1993-0631 |
| language | eng |
| recordid | cdi_wanfang_journals_zgazyj200801001 |
| source | EZB Electronic Journals Library |
| subjects | Basic Research Medicine Medicine & Public Health 人乳头状瘤病毒 细胞增殖 蛋白质 |
| title | Inhibitory Effect of Nordy on HPV16 E6 Gene in Human Immortalized Endocervical Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T18%3A05%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20Effect%20of%20Nordy%20on%20HPV16%20E6%20Gene%20in%20Human%20Immortalized%20Endocervical%20Cells&rft.jtitle=Chinese%20journal%20of%20cancer%20research&rft.au=%E9%99%88%E7%8E%AE%20%E8%B5%B5%E6%B6%8C%20%E5%8D%9E%E4%BF%AE%E6%AD%A6&rft.date=2008-03-01&rft.volume=20&rft.issue=1&rft.spage=1&rft.epage=4&rft.pages=1-4&rft.issn=1000-9604&rft.eissn=1993-0631&rft_id=info:doi/10.1007/s11670-008-0001-8&rft_dat=%3Cwanfang_jour_cross%3Ezgazyj200801001%3C/wanfang_jour_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=26680611&rft_wanfj_id=zgazyj200801001&rfr_iscdi=true |