Construction of eukaryotic expression vector with granulocyte-macrophage colony-stimulating factor gene

Construction of eukaryotic expression vector with granulocyte-macrophage colony-stimulating factor gene

R73; Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by...

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Veröffentlicht in:Chinese journal of cancer research 2000-06, Vol.12 (2), p.125-127
Hauptverfasser: Zheng, Qiu-hong, Zheng, Tian-rong, Xie, Yun-qing, Lu, Lin, Chen, Hui
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container_issue 2
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container_title Chinese journal of cancer research
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creator Zheng, Qiu-hong
Zheng, Tian-rong
Xie, Yun-qing
Lu, Lin
Chen, Hui
description R73; Objective: To construct the eukaryotic expression vector that express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) gene for making highly express in mammalian cells. Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.
doi_str_mv 10.1007/BF02983437
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Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.</description><identifier>ISSN: 1000-9604</identifier><identifier>DOI: 10.1007/BF02983437</identifier><language>eng</language><publisher>Department of Tumor Molecular Biology, Fujian Tumor Hospital, Fuzhou 350014, China</publisher><ispartof>Chinese journal of cancer research, 2000-06, Vol.12 (2), p.125-127</ispartof><rights>Copyright © Wanfang Data Co. Ltd. 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Methods: Extract totally RNA from the induced human fetal lung (HFL) cell line. HGM-CSF cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR), and then directionally subcloned into the HindIII and EcoRI site on the pcDNA3.1 plasmid, which was controlled by the CMV promoter, to form the recombinant expressing vector pcDNA3.1-GM-CSF. Results: The PCR amplification was identified and the sequence was analyzed, the results showed that hGM-CSF was properly inserted into the vector and the sequence was correct.</abstract><pub>Department of Tumor Molecular Biology, Fujian Tumor Hospital, Fuzhou 350014, China</pub><doi>10.1007/BF02983437</doi><tpages>3</tpages></addata></record>
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title Construction of eukaryotic expression vector with granulocyte-macrophage colony-stimulating factor gene
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