Effect of levocarnitine on TIMP-1,ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect

Objective:To study the effect of levocarnitine(L-CN) on tissue inhibitor of metalloproteinase-1(TIMP-1) and intercellular adhesion molecule-1(ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells.Methods:Highfat diet feeding and intrape...

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Veröffentlicht in:Asian Pacific journal of tropical medicine 2016-03, Vol.9 (3), p.264-268
Hauptverfasser: Wang, Shi-Xun, Tan, Lei, Wang, Jian, Zhong, Jing-Quan
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container_start_page 264
container_title Asian Pacific journal of tropical medicine
container_volume 9
creator Wang, Shi-Xun
Tan, Lei
Wang, Jian
Zhong, Jing-Quan
description Objective:To study the effect of levocarnitine(L-CN) on tissue inhibitor of metalloproteinase-1(TIMP-1) and intercellular adhesion molecule-1(ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells.Methods:Highfat diet feeding and intraperitoneal injection of pituitrin was performed on rats in model group and CHD Model of rats was built.Rats with successful model-building were selected and divided into L-CN group and Ctrl group randomly.Rats in L-CN group were given L-CN treatment,with intraperitoneal injection of 200 mg·kg-1?d-1 and successive administration for 3 d.Rats in Ctrl group were given equal volumes of normal saline.Blood was collected from carotid artery at different time and expression quantity of creatine kinase-MB(CK-MB) and Troponin Ⅰ(Tn Ⅰ)in serum was detected.Rats in each group were put to death and were separated to obtain the myocardial tissue.Real-time PCR and Western Blotting hybridization were performed to detect the TIMP-1.ICAM-1 expression in myocardial tissue in each group.Statistical analysis was employed to explore the expression changes of TIMP-1 and ICAM-1.and ELISA test was used lo analyse the expression changes of myocardial necrosis marker- CK-MB and Tn I to learn the effect of L-CN and its myocardial protective effect.Results:The total cholesterol,triglyceride and blood glucose levels of rats in model group were significantly higher than that in control group,which indicated that due to high-fat diet feeding,blood lipid of rats in model group was obviously higher than that in control group.In myocardial tissue of rats in model group,TIMP-I level significantly reduced and ICAM-1 level significantly increased(P<0.0l).In model group,after L-CN treatment.TTMP-I level had double increase,while ICAM-1 level had 43%of decrease in L-CN group compared with Ctrl group.After L-CN intervention treatment.CK-MB and Tn Ⅰ content in L-CN group relatively reduced compared with Ctrl group.The difference among groups was obvious(P<0.01).Condusions:L-CN could increase the TTMP-I expression level and inhibit the ICAM-1 expression level.L-CN has a certain myocardial protective effect
doi_str_mv 10.1016/j.apjtm.2016.01.025
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Production and hosting by Elsevier B.V. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-4fbf822ed01b6cbacc1da3575b229dd014fea11f45012cefdf38ca780ef7f7393</citedby><cites>FETCH-LOGICAL-c586t-4fbf822ed01b6cbacc1da3575b229dd014fea11f45012cefdf38ca780ef7f7393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71792X/71792X.jpg</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1995764516000262$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26972400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Shi-Xun</creatorcontrib><creatorcontrib>Tan, Lei</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><creatorcontrib>Zhong, Jing-Quan</creatorcontrib><title>Effect of levocarnitine on TIMP-1,ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect</title><title>Asian Pacific journal of tropical medicine</title><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><description>Objective:To study the effect of levocarnitine(L-CN) on tissue inhibitor of metalloproteinase-1(TIMP-1) and intercellular adhesion molecule-1(ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells.Methods:Highfat diet feeding and intraperitoneal injection of pituitrin was performed on rats in model group and CHD Model of rats was built.Rats with successful model-building were selected and divided into L-CN group and Ctrl group randomly.Rats in L-CN group were given L-CN treatment,with intraperitoneal injection of 200 mg·kg-1?d-1 and successive administration for 3 d.Rats in Ctrl group were given equal volumes of normal saline.Blood was collected from carotid artery at different time and expression quantity of creatine kinase-MB(CK-MB) and Troponin Ⅰ(Tn Ⅰ)in serum was detected.Rats in each group were put to death and were separated to obtain the myocardial tissue.Real-time PCR and Western Blotting hybridization were performed to detect the TIMP-1.ICAM-1 expression in myocardial tissue in each group.Statistical analysis was employed to explore the expression changes of TIMP-1 and ICAM-1.and ELISA test was used lo analyse the expression changes of myocardial necrosis marker- CK-MB and Tn I to learn the effect of L-CN and its myocardial protective effect.Results:The total cholesterol,triglyceride and blood glucose levels of rats in model group were significantly higher than that in control group,which indicated that due to high-fat diet feeding,blood lipid of rats in model group was obviously higher than that in control group.In myocardial tissue of rats in model group,TIMP-I level significantly reduced and ICAM-1 level significantly increased(P&amp;lt;0.0l).In model group,after L-CN treatment.TTMP-I level had double increase,while ICAM-1 level had 43%of decrease in L-CN group compared with Ctrl group.After L-CN intervention treatment.CK-MB and Tn Ⅰ content in L-CN group relatively reduced compared with Ctrl group.The difference among groups was obvious(P&amp;lt;0.01).Condusions:L-CN could increase the TTMP-I expression level and inhibit the ICAM-1 expression level.L-CN has a certain myocardial protective effect</description><subject>Coronary heart disease</subject><subject>disease;TIMP-1;ICAM-1</subject><subject>heart</subject><subject>ICAM-1</subject><subject>Levocarnitine</subject><subject>Levocarnitine;Coronary</subject><subject>TIMP-1</subject><issn>1995-7645</issn><issn>2352-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkUtvEzEUhUcIRKPSX4CEvESICX7O2AsWqCpQqRUsytry2NeNR4md2pO2qfjxOEnDFrzx67v3XJ3TNG8JnhNMuk_j3KzHaTWn9TLHZI6peNHMKBO05YR3L5sZUUq0fcfFSXNWyojrYlSpnr1uTminesoxnjW_L7wHO6Hk0RLukzU5hilEQCmim8vrny35eHn-5bolCB7XGUoJ9aPC2UwFPYRpgWzKKZq8RQsweUIuFDAFkIkOhcqstrumLpglWuc0Va1dB9irvmleebMscPa8nza_vl7cnH9vr358q6pXrRWym1ruBy8pBYfJ0NnBWEucYaIXA6XK1VfuwRDiucCEWvDOM2lNLzH43vdMsdPmw6Hvg4nexFs9pk2OVVFvs9tun5407HzErNZX-P0BruPebaBMehWKheXSREibokmvsBKko_g_0J5xKjtOKsoOqM2plAxer3NYVds0wXqXqB71PlG9m0Rjomuiterds8BmWIH7W3PMrwKfDwBU--4DZF1sgGjBhVwN1i6Ffwgcx1qkeHsXqjdHDakkl1QqgbnkSnAuBdmfCPsDG7bCZQ</recordid><startdate>20160301</startdate><enddate>20160301</enddate><creator>Wang, 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(COJ)</collection><jtitle>Asian Pacific journal of tropical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shi-Xun</au><au>Tan, Lei</au><au>Wang, Jian</au><au>Zhong, Jing-Quan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of levocarnitine on TIMP-1,ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect</atitle><jtitle>Asian Pacific journal of tropical medicine</jtitle><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><date>2016-03-01</date><risdate>2016</risdate><volume>9</volume><issue>3</issue><spage>264</spage><epage>268</epage><pages>264-268</pages><issn>1995-7645</issn><eissn>2352-4146</eissn><abstract>Objective:To study the effect of levocarnitine(L-CN) on tissue inhibitor of metalloproteinase-1(TIMP-1) and intercellular adhesion molecule-1(ICAM-1) expression of rats with coronary heart disease and evaluate the protective effect of L-CN on myocardial cells.Methods:Highfat diet feeding and intraperitoneal injection of pituitrin was performed on rats in model group and CHD Model of rats was built.Rats with successful model-building were selected and divided into L-CN group and Ctrl group randomly.Rats in L-CN group were given L-CN treatment,with intraperitoneal injection of 200 mg·kg-1?d-1 and successive administration for 3 d.Rats in Ctrl group were given equal volumes of normal saline.Blood was collected from carotid artery at different time and expression quantity of creatine kinase-MB(CK-MB) and Troponin Ⅰ(Tn Ⅰ)in serum was detected.Rats in each group were put to death and were separated to obtain the myocardial tissue.Real-time PCR and Western Blotting hybridization were performed to detect the TIMP-1.ICAM-1 expression in myocardial tissue in each group.Statistical analysis was employed to explore the expression changes of TIMP-1 and ICAM-1.and ELISA test was used lo analyse the expression changes of myocardial necrosis marker- CK-MB and Tn I to learn the effect of L-CN and its myocardial protective effect.Results:The total cholesterol,triglyceride and blood glucose levels of rats in model group were significantly higher than that in control group,which indicated that due to high-fat diet feeding,blood lipid of rats in model group was obviously higher than that in control group.In myocardial tissue of rats in model group,TIMP-I level significantly reduced and ICAM-1 level significantly increased(P&amp;lt;0.0l).In model group,after L-CN treatment.TTMP-I level had double increase,while ICAM-1 level had 43%of decrease in L-CN group compared with Ctrl group.After L-CN intervention treatment.CK-MB and Tn Ⅰ content in L-CN group relatively reduced compared with Ctrl group.The difference among groups was obvious(P&amp;lt;0.01).Condusions:L-CN could increase the TTMP-I expression level and inhibit the ICAM-1 expression level.L-CN has a certain myocardial protective effect</abstract><cop>India</cop><pub>Elsevier B.V</pub><pmid>26972400</pmid><doi>10.1016/j.apjtm.2016.01.025</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1995-7645
ispartof Asian Pacific journal of tropical medicine, 2016-03, Vol.9 (3), p.264-268
issn 1995-7645
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language eng
recordid cdi_wanfang_journals_yrdyyzz_e201603012
source Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Coronary heart disease
disease
TIMP-1
ICAM-1
heart
ICAM-1
Levocarnitine
Levocarnitine
Coronary
TIMP-1
title Effect of levocarnitine on TIMP-1,ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect
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