Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus
Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN)...
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| Veröffentlicht in: | Asian Pacific journal of tropical medicine 2016-01, Vol.9 (1), p.16-19 |
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| creator | Joung, Dae-Ki Lee, Young-Seob Han, Sin-Hee Lee, Sang-Won Cha, Seon-Woo Mun, Su-Hyun Kong, Ryong Kang, Ok-Hwa Song, Ho-Jun Shin, Dong-Won Kwon, Dong-Yeul |
| description | Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(S.aureus).Also,transmission electron microscopy was used to monitor survival characteristics and changes in S.aureus morphology.Results:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,respectively.PGN(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on S.aureus.Conclusions:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA isolates.The results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting ATPase.S.aureus PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with PGN.These findings may be validated for the development of antibacterial agent for low MRSA resistance. |
| doi_str_mv | 10.1016/j.apjtm.2015.12.004 |
| format | Article |
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Production and hosting by Elsevier B.V. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-436db40a1e832b899db1e662829d2f2d6b96c8058dbc4277a0477b3beedf51623</citedby><cites>FETCH-LOGICAL-c586t-436db40a1e832b899db1e662829d2f2d6b96c8058dbc4277a0477b3beedf51623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71792X/71792X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.apjtm.2015.12.004$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26851780$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joung, Dae-Ki</creatorcontrib><creatorcontrib>Lee, Young-Seob</creatorcontrib><creatorcontrib>Han, Sin-Hee</creatorcontrib><creatorcontrib>Lee, Sang-Won</creatorcontrib><creatorcontrib>Cha, Seon-Woo</creatorcontrib><creatorcontrib>Mun, Su-Hyun</creatorcontrib><creatorcontrib>Kong, Ryong</creatorcontrib><creatorcontrib>Kang, Ok-Hwa</creatorcontrib><creatorcontrib>Song, Ho-Jun</creatorcontrib><creatorcontrib>Shin, Dong-Won</creatorcontrib><creatorcontrib>Kwon, Dong-Yeul</creatorcontrib><title>Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus</title><title>Asian Pacific journal of tropical medicine</title><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><description>Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(S.aureus).Also,transmission electron microscopy was used to monitor survival characteristics and changes in S.aureus morphology.Results:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,respectively.PGN(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on S.aureus.Conclusions:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA isolates.The results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting ATPase.S.aureus PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with PGN.These findings may be validated for the development of antibacterial agent for low MRSA resistance.</description><subject>agent;ATPase</subject><subject>ATPase inhibitor</subject><subject>aureus;Membrane</subject><subject>inhibitor;Peptidoglycan</subject><subject>Iuteolin;Methicillin-resistant</subject><subject>Luteolin</subject><subject>Membrane permeabilizing agent</subject><subject>Methicillin-resistant Staphylococcus aureus</subject><subject>Peptidoglycan</subject><subject>permeabilizing</subject><subject>Staphylococcus</subject><subject>Staphylococcus 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Dong-Won</au><au>Kwon, Dong-Yeul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus</atitle><jtitle>Asian Pacific journal of tropical medicine</jtitle><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><date>2016-01-01</date><risdate>2016</risdate><volume>9</volume><issue>1</issue><spage>16</spage><epage>19</epage><pages>16-19</pages><issn>1995-7645</issn><eissn>2352-4146</eissn><abstract>Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(S.aureus).Also,transmission electron microscopy was used to monitor survival characteristics and changes in S.aureus morphology.Results:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,respectively.PGN(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on S.aureus.Conclusions:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA isolates.The results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting ATPase.S.aureus PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with PGN.These findings may be validated for the development of antibacterial agent for low MRSA resistance.</abstract><cop>India</cop><pub>Elsevier B.V</pub><pmid>26851780</pmid><doi>10.1016/j.apjtm.2015.12.004</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | agent ATPase ATPase inhibitor aureus Membrane inhibitor Peptidoglycan Iuteolin Methicillin-resistant Luteolin Membrane permeabilizing agent Methicillin-resistant Staphylococcus aureus Peptidoglycan permeabilizing Staphylococcus Staphylococcus aureus |
| title | Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus |
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