Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells
To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60. 50 mL cord blood sample was obtained from infants delivered by full term...
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| Veröffentlicht in: | Asian Pacific journal of tropical medicine 2014-08, Vol.7 (8), p.659-662 |
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| container_title | Asian Pacific journal of tropical medicine |
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| creator | Qu, Hui-Qing Zhou, Xiao-Sheng Zhou, Xiao-Long Wang, Jian |
| description | To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.
50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.
Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.
Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK. |
| doi_str_mv | 10.1016/S1995-7645(14)60111-5 |
| format | Article |
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50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.
Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.
Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.</description><identifier>ISSN: 1995-7645</identifier><identifier>EISSN: 2352-4146</identifier><identifier>DOI: 10.1016/S1995-7645(14)60111-5</identifier><identifier>PMID: 25149382</identifier><language>eng</language><publisher>India: Department of Blood Transfusion, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Department of Neurology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Pharmaceutical Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Laboratory Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China</publisher><subject>Apoptosis - physiology ; Cell Differentiation - physiology ; Cell Proliferation - physiology ; Coculture Techniques ; Cytokine-Induced Killer Cells - cytology ; Cytokines - metabolism ; Dendritic Cells - cytology ; Female ; Fetal Blood - cytology ; HL-60 Cells ; Humans ; Leukemia ; Phenotype</subject><ispartof>Asian Pacific journal of tropical medicine, 2014-08, Vol.7 (8), p.659-662</ispartof><rights>Copyright © 2014 Hainan Medical College. Published by Elsevier B.V. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c485t-9389bd8fd16e92b440cf53fc5e1d20f22d0a0948bef7a57a4a3554f77c447e6f3</citedby><cites>FETCH-LOGICAL-c485t-9389bd8fd16e92b440cf53fc5e1d20f22d0a0948bef7a57a4a3554f77c447e6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71792X/71792X.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25149382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qu, Hui-Qing</creatorcontrib><creatorcontrib>Zhou, Xiao-Sheng</creatorcontrib><creatorcontrib>Zhou, Xiao-Long</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><title>Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells</title><title>Asian Pacific journal of tropical medicine</title><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><description>To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.
50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.
Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.
Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.</description><subject>Apoptosis - physiology</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Proliferation - physiology</subject><subject>Coculture Techniques</subject><subject>Cytokine-Induced Killer Cells - cytology</subject><subject>Cytokines - metabolism</subject><subject>Dendritic Cells - cytology</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Leukemia</subject><subject>Phenotype</subject><issn>1995-7645</issn><issn>2352-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9vEzEQxS0EolHJRwBZ4lJQFzz-t-tjFUqpiMQBOHGwnF27dbuxE3tXKP30eJPQHjsXH-b33oznIfQWyCcgID__BKVEVUsuzoB_kAQAKvECzSgTtOLA5Us0e0RO0DznO1KKUaVq9hqdUAFcsYbO0J9L52w74Ojwl0W1uP6OW9v3OAY83Fq8SbH3ziYz-BjOsdnEzRCzz9iEDne-SJMNg9-3J4vejvd27c3eJL9Br5zps50f31P0--vlr8W3avnj6npxsaxa3oihKouoVde4DqRVdMU5aZ1grhUWOkocpR0xRPFmZV1tRG24YUJwV9ct57WVjp2ijwffvyY4E270XRxTKBP1LnW73cODtpQAJw0BWuCzA1z-th1tHvTa52ldE2wcswYpCVNsus_zKDRQaEGeR4UQhVYNL6g4oG2KOSfr9Cb5tUk7DURP6ep9unqKTgPX-3S1KLp3xxHjam27R9X_LAvw_mh8G8PN1pdDPDkTRphsqGL_ANWFqZg</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Qu, Hui-Qing</creator><creator>Zhou, Xiao-Sheng</creator><creator>Zhou, Xiao-Long</creator><creator>Wang, Jian</creator><general>Department of Blood Transfusion, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Department of Neurology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Pharmaceutical Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Laboratory Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20140801</creationdate><title>Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells</title><author>Qu, Hui-Qing ; Zhou, Xiao-Sheng ; Zhou, Xiao-Long ; Wang, Jian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c485t-9389bd8fd16e92b440cf53fc5e1d20f22d0a0948bef7a57a4a3554f77c447e6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis - physiology</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Proliferation - physiology</topic><topic>Coculture Techniques</topic><topic>Cytokine-Induced Killer Cells - cytology</topic><topic>Cytokines - metabolism</topic><topic>Dendritic Cells - cytology</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Leukemia</topic><topic>Phenotype</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qu, Hui-Qing</creatorcontrib><creatorcontrib>Zhou, Xiao-Sheng</creatorcontrib><creatorcontrib>Zhou, Xiao-Long</creatorcontrib><creatorcontrib>Wang, Jian</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Asian Pacific journal of tropical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qu, Hui-Qing</au><au>Zhou, Xiao-Sheng</au><au>Zhou, Xiao-Long</au><au>Wang, Jian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells</atitle><jtitle>Asian Pacific journal of tropical medicine</jtitle><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>7</volume><issue>8</issue><spage>659</spage><epage>662</epage><pages>659-662</pages><issn>1995-7645</issn><eissn>2352-4146</eissn><abstract>To observe the effect of co-culture cytokine-induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60.
50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non-adherent cells were collectedfor the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay.
Compared with CIKs, the co-cultured DC-CIKs presented a markedly higher proliferation and killing activity.
Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.</abstract><cop>India</cop><pub>Department of Blood Transfusion, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Department of Neurology, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Pharmaceutical Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China%Laboratory Department, Affiliated Hospital of Binzhou Medical College, Binzhou 256603, China</pub><pmid>25149382</pmid><doi>10.1016/S1995-7645(14)60111-5</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1995-7645 |
| ispartof | Asian Pacific journal of tropical medicine, 2014-08, Vol.7 (8), p.659-662 |
| issn | 1995-7645 2352-4146 |
| language | eng |
| recordid | cdi_wanfang_journals_yrdyyzz_e201408012 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Apoptosis - physiology Cell Differentiation - physiology Cell Proliferation - physiology Coculture Techniques Cytokine-Induced Killer Cells - cytology Cytokines - metabolism Dendritic Cells - cytology Female Fetal Blood - cytology HL-60 Cells Humans Leukemia Phenotype |
| title | Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells |
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