Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro

Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro

Objective:To investigate the effect of the spinal cord extracts(SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells(NSCs) and the expressions of mRNA ofNotch1 as well as ofHes1 in this processin vitro.Methods:The experiment was conducted in4 different mediu...

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Veröffentlicht in:亚太热带医药杂志（英文版） 2014 (7), p.562-567
Hauptverfasser: Qing-Zhong Zhou, Ge Zhang, Hai-Bo Long, Fei Lei, Fei Ye, Xu-Feng Jia, Yun-Long Zhou, Jian-Ping Kang, Da-Xiong Feng
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container_title 亚太热带医药杂志（英文版）
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creator Qing-Zhong Zhou
Ge Zhang
Hai-Bo Long
Fei Lei
Fei Ye
Xu-Feng Jia
Yun-Long Zhou
Jian-Ping Kang
Da-Xiong Feng
description Objective:To investigate the effect of the spinal cord extracts(SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells(NSCs) and the expressions of mRNA ofNotch1 as well as ofHes1 in this processin vitro.Methods:The experiment was conducted in4 different mediums:NSCs+PBS(GroupA-blank control group),NSCs+SCE with healthySD rats(GroupB-normal control group),NSCs+SCE withSD rats receiving sham-operation treatment (GroupC-sham-operation group) andNSCs+SCE withSCIs rats(GroupD- paraplegic group). Proliferative abilities of4 different groups were analyzed byMTT chromatometry after co-culture for1,2,3,4 and5 d, respectively.The expressions ofNotch1 andHes1 mRNA were also detected withRT-PCR after co-culture for24 and48 h, respectively.Results:After co-culture for1,2,3, 4 and5 d respectively, theMTT values of groupD were significantly higher than those of group A, groupB and groupC(P0.05).Both the expressions ofNotch1 andHes1 mRNA of groupD were significantly higher than those of other3 groups after co-culture for24 h and48 h as well(P0.05).There was no difference in expressions ofNotch1 andHes1 mRNA between24 h and48 h treatment in groupD.Conclusions:SCE could promote the proliferation ofNSCs.It is demonstrated that the microenvironment ofSCI may promote the proliferation ofNSCs.Besides,SCE could increase the expression ofNotch1 andHes1 mRNA of NSC.It can be concluded that theNotch signaling pathway activation is one of the mechanisms that locally injured microenvironment contributes to the proliferation ofENSC afterSCIs.This process may be performed by up-regulating the expressions ofNotch1 andHes1gene.
doi_str_mv 10.1016/S1995-7645(14)60094-8
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fullrecord <record><control><sourceid>wanfang_jour</sourceid><recordid>TN_cdi_wanfang_journals_yrdyyzz_e201407011</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>yrdyyzz_e201407011</wanfj_id><sourcerecordid>yrdyyzz_e201407011</sourcerecordid><originalsourceid>FETCH-wanfang_journals_yrdyyzz_e2014070113</originalsourceid><addsrcrecordid>eNqVjMFOwzAQRH0AiQr6CUh7BKSA3aQJPqMiTlzgHhln3Tpy7WjtUNxv4KMxEkLiyF5mZ3bnMXYp-K3gor17EVKuq65t1leiuW45l011f8IWv_EZW8Y48jL1SsquXrDPjTGoEwQDcbJeOdCBBsCPREqnCMokpD8n68eZMgQPEwVnDZJKtrhCKBvg_o1y8FaDx5lKKSbcg0bnCswP8ByS3kG022_gpNLuoLL18G4ThQt2apSLuPzRc3bzuHl9eKoOyhvlt_0YZiq92Gcacj4ee1xx0fCOC1H_6_kLw89iIQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro</title><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Qing-Zhong Zhou ; Ge Zhang ; Hai-Bo Long ; Fei Lei ; Fei Ye ; Xu-Feng Jia ; Yun-Long Zhou ; Jian-Ping Kang ; Da-Xiong Feng</creator><creatorcontrib>Qing-Zhong Zhou ; Ge Zhang ; Hai-Bo Long ; Fei Lei ; Fei Ye ; Xu-Feng Jia ; Yun-Long Zhou ; Jian-Ping Kang ; Da-Xiong Feng</creatorcontrib><description>Objective:To investigate the effect of the spinal cord extracts(SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells(NSCs) and the expressions of mRNA ofNotch1 as well as ofHes1 in this processin vitro.Methods:The experiment was conducted in4 different mediums:NSCs+PBS(GroupA-blank control group),NSCs+SCE with healthySD rats(GroupB-normal control group),NSCs+SCE withSD rats receiving sham-operation treatment (GroupC-sham-operation group) andNSCs+SCE withSCIs rats(GroupD- paraplegic group). Proliferative abilities of4 different groups were analyzed byMTT chromatometry after co-culture for1,2,3,4 and5 d, respectively.The expressions ofNotch1 andHes1 mRNA were also detected withRT-PCR after co-culture for24 and48 h, respectively.Results:After co-culture for1,2,3, 4 and5 d respectively, theMTT values of groupD were significantly higher than those of group A, groupB and groupC(P&lt;0.05).However, there were no significantly differences regardingMTT values between groupA, groupB and groupC after co-culture for1,2,3,4 and5 d, respectively (P&gt;0.05).Both the expressions ofNotch1 andHes1 mRNA of groupD were significantly higher than those of other3 groups after co-culture for24 h and48 h as well(P&lt;0.05).But there was no difference oin expressions ofNotch1 andHes1 mRNA among groupA, groupB and groupC after co-culture for24 h and48 h(P&gt;0.05).There was no difference in expressions ofNotch1 andHes1 mRNA between24 h and48 h treatment in groupD.Conclusions:SCE could promote the proliferation ofNSCs.It is demonstrated that the microenvironment ofSCI may promote the proliferation ofNSCs.Besides,SCE could increase the expression ofNotch1 andHes1 mRNA of NSC.It can be concluded that theNotch signaling pathway activation is one of the mechanisms that locally injured microenvironment contributes to the proliferation ofENSC afterSCIs.This process may be performed by up-regulating the expressions ofNotch1 andHes1gene.</description><identifier>ISSN: 1995-7645</identifier><identifier>DOI: 10.1016/S1995-7645(14)60094-8</identifier><language>eng</language><publisher>Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China%Department of Spine Surgery, Popele's Hospital of Luzhou, Luzhou 646000, China%Department of Spine Surgery, Affiliated TCM Hospital of Luzhou Medical College, Luzhou 646000, China</publisher><ispartof>亚太热带医药杂志（英文版）, 2014 (7), p.562-567</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/yrdyyzz-e/yrdyyzz-e.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Qing-Zhong Zhou</creatorcontrib><creatorcontrib>Ge Zhang</creatorcontrib><creatorcontrib>Hai-Bo Long</creatorcontrib><creatorcontrib>Fei Lei</creatorcontrib><creatorcontrib>Fei Ye</creatorcontrib><creatorcontrib>Xu-Feng Jia</creatorcontrib><creatorcontrib>Yun-Long Zhou</creatorcontrib><creatorcontrib>Jian-Ping Kang</creatorcontrib><creatorcontrib>Da-Xiong Feng</creatorcontrib><title>Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro</title><title>亚太热带医药杂志（英文版）</title><description>Objective:To investigate the effect of the spinal cord extracts(SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells(NSCs) and the expressions of mRNA ofNotch1 as well as ofHes1 in this processin vitro.Methods:The experiment was conducted in4 different mediums:NSCs+PBS(GroupA-blank control group),NSCs+SCE with healthySD rats(GroupB-normal control group),NSCs+SCE withSD rats receiving sham-operation treatment (GroupC-sham-operation group) andNSCs+SCE withSCIs rats(GroupD- paraplegic group). Proliferative abilities of4 different groups were analyzed byMTT chromatometry after co-culture for1,2,3,4 and5 d, respectively.The expressions ofNotch1 andHes1 mRNA were also detected withRT-PCR after co-culture for24 and48 h, respectively.Results:After co-culture for1,2,3, 4 and5 d respectively, theMTT values of groupD were significantly higher than those of group A, groupB and groupC(P&lt;0.05).However, there were no significantly differences regardingMTT values between groupA, groupB and groupC after co-culture for1,2,3,4 and5 d, respectively (P&gt;0.05).Both the expressions ofNotch1 andHes1 mRNA of groupD were significantly higher than those of other3 groups after co-culture for24 h and48 h as well(P&lt;0.05).But there was no difference oin expressions ofNotch1 andHes1 mRNA among groupA, groupB and groupC after co-culture for24 h and48 h(P&gt;0.05).There was no difference in expressions ofNotch1 andHes1 mRNA between24 h and48 h treatment in groupD.Conclusions:SCE could promote the proliferation ofNSCs.It is demonstrated that the microenvironment ofSCI may promote the proliferation ofNSCs.Besides,SCE could increase the expression ofNotch1 andHes1 mRNA of NSC.It can be concluded that theNotch signaling pathway activation is one of the mechanisms that locally injured microenvironment contributes to the proliferation ofENSC afterSCIs.This process may be performed by up-regulating the expressions ofNotch1 andHes1gene.</description><issn>1995-7645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqVjMFOwzAQRH0AiQr6CUh7BKSA3aQJPqMiTlzgHhln3Tpy7WjtUNxv4KMxEkLiyF5mZ3bnMXYp-K3gor17EVKuq65t1leiuW45l011f8IWv_EZW8Y48jL1SsquXrDPjTGoEwQDcbJeOdCBBsCPREqnCMokpD8n68eZMgQPEwVnDZJKtrhCKBvg_o1y8FaDx5lKKSbcg0bnCswP8ByS3kG022_gpNLuoLL18G4ThQt2apSLuPzRc3bzuHl9eKoOyhvlt_0YZiq92Gcacj4ee1xx0fCOC1H_6_kLw89iIQ</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Qing-Zhong Zhou</creator><creator>Ge Zhang</creator><creator>Hai-Bo Long</creator><creator>Fei Lei</creator><creator>Fei Ye</creator><creator>Xu-Feng Jia</creator><creator>Yun-Long Zhou</creator><creator>Jian-Ping Kang</creator><creator>Da-Xiong Feng</creator><general>Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China%Department of Spine Surgery, Popele's Hospital of Luzhou, Luzhou 646000, China%Department of Spine Surgery, Affiliated TCM Hospital of Luzhou Medical College, Luzhou 646000, China</general><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2014</creationdate><title>Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro</title><author>Qing-Zhong Zhou ; Ge Zhang ; Hai-Bo Long ; Fei Lei ; Fei Ye ; Xu-Feng Jia ; Yun-Long Zhou ; Jian-Ping Kang ; Da-Xiong Feng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-wanfang_journals_yrdyyzz_e2014070113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qing-Zhong Zhou</creatorcontrib><creatorcontrib>Ge Zhang</creatorcontrib><creatorcontrib>Hai-Bo Long</creatorcontrib><creatorcontrib>Fei Lei</creatorcontrib><creatorcontrib>Fei Ye</creatorcontrib><creatorcontrib>Xu-Feng Jia</creatorcontrib><creatorcontrib>Yun-Long Zhou</creatorcontrib><creatorcontrib>Jian-Ping Kang</creatorcontrib><creatorcontrib>Da-Xiong Feng</creatorcontrib><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>亚太热带医药杂志（英文版）</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qing-Zhong Zhou</au><au>Ge Zhang</au><au>Hai-Bo Long</au><au>Fei Lei</au><au>Fei Ye</au><au>Xu-Feng Jia</au><au>Yun-Long Zhou</au><au>Jian-Ping Kang</au><au>Da-Xiong Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro</atitle><jtitle>亚太热带医药杂志（英文版）</jtitle><date>2014</date><risdate>2014</risdate><issue>7</issue><spage>562</spage><epage>567</epage><pages>562-567</pages><issn>1995-7645</issn><abstract>Objective:To investigate the effect of the spinal cord extracts(SCE) after spinal cord injuries (SCIs) on the proliferation of rat embryonic neural stem cells(NSCs) and the expressions of mRNA ofNotch1 as well as ofHes1 in this processin vitro.Methods:The experiment was conducted in4 different mediums:NSCs+PBS(GroupA-blank control group),NSCs+SCE with healthySD rats(GroupB-normal control group),NSCs+SCE withSD rats receiving sham-operation treatment (GroupC-sham-operation group) andNSCs+SCE withSCIs rats(GroupD- paraplegic group). Proliferative abilities of4 different groups were analyzed byMTT chromatometry after co-culture for1,2,3,4 and5 d, respectively.The expressions ofNotch1 andHes1 mRNA were also detected withRT-PCR after co-culture for24 and48 h, respectively.Results:After co-culture for1,2,3, 4 and5 d respectively, theMTT values of groupD were significantly higher than those of group A, groupB and groupC(P&lt;0.05).However, there were no significantly differences regardingMTT values between groupA, groupB and groupC after co-culture for1,2,3,4 and5 d, respectively (P&gt;0.05).Both the expressions ofNotch1 andHes1 mRNA of groupD were significantly higher than those of other3 groups after co-culture for24 h and48 h as well(P&lt;0.05).But there was no difference oin expressions ofNotch1 andHes1 mRNA among groupA, groupB and groupC after co-culture for24 h and48 h(P&gt;0.05).There was no difference in expressions ofNotch1 andHes1 mRNA between24 h and48 h treatment in groupD.Conclusions:SCE could promote the proliferation ofNSCs.It is demonstrated that the microenvironment ofSCI may promote the proliferation ofNSCs.Besides,SCE could increase the expression ofNotch1 andHes1 mRNA of NSC.It can be concluded that theNotch signaling pathway activation is one of the mechanisms that locally injured microenvironment contributes to the proliferation ofENSC afterSCIs.This process may be performed by up-regulating the expressions ofNotch1 andHes1gene.</abstract><pub>Department of Spine Surgery, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China%Department of Spine Surgery, Popele's Hospital of Luzhou, Luzhou 646000, China%Department of Spine Surgery, Affiliated TCM Hospital of Luzhou Medical College, Luzhou 646000, China</pub><doi>10.1016/S1995-7645(14)60094-8</doi></addata></record>
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title Effect of spinal cord extracts after spinal cord injury on proliferation of rat embryonic neural stem cells and Notch signal pathwayin vitro
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