Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast
Objective:To explore tissue factor(TF)expression and methylation regulation in differentiation of human embryonic stem cells(hESCs)into trophoblast.Methods:Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4(BMP4).Expression of gene,protein of TF and DNA methylation...
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| Veröffentlicht in: | Asian Pacific journal of tropical medicine 2014-07, Vol.7 (7), p.557-561 |
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| creator | Liu, Lin-Xin Zeng, Hui Liu, En-Yi Chen, Fang-Ping |
| description | Objective:To explore tissue factor(TF)expression and methylation regulation in differentiation of human embryonic stem cells(hESCs)into trophoblast.Methods:Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4(BMP4).Expression of gene,protein of TF and DNA methylation at different time points during induction process was detected by RTPCT,Western blot,flow cytometry and MSP-PCR method.Results:The expression of mRNA,protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells,semi methylation-semi non methylation expression appeared at TF DNA promoter region,and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of TF.Conclusions:It shows that during differentiation of hESCs into trophoblast,the differential expression of TF is related with DNA methylation level,and it is changed with the methylation or non methylated degree.It provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development. |
| doi_str_mv | 10.1016/S1995-7645(14)60093-6 |
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Published by Elsevier B.V. All rights reserved.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c541t-9637be6c5462f943bec22bc9c066730c0dbf19538941a1a1b8f32e8f3248cf813</citedby><cites>FETCH-LOGICAL-c541t-9637be6c5462f943bec22bc9c066730c0dbf19538941a1a1b8f32e8f3248cf813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71792X/71792X.jpg</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1995-7645(14)60093-6$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25063286$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Lin-Xin</creatorcontrib><creatorcontrib>Zeng, Hui</creatorcontrib><creatorcontrib>Liu, En-Yi</creatorcontrib><creatorcontrib>Chen, Fang-Ping</creatorcontrib><title>Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast</title><title>Asian Pacific journal of tropical medicine</title><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><description>Objective:To explore tissue factor(TF)expression and methylation regulation in differentiation of human embryonic stem cells(hESCs)into trophoblast.Methods:Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4(BMP4).Expression of gene,protein of TF and DNA methylation at different time points during induction process was detected by RTPCT,Western blot,flow cytometry and MSP-PCR method.Results:The expression of mRNA,protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells,semi methylation-semi non methylation expression appeared at TF DNA promoter region,and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of TF.Conclusions:It shows that during differentiation of hESCs into trophoblast,the differential expression of TF is related with DNA methylation level,and it is changed with the methylation or non methylated degree.It provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development.</description><subject>Animals</subject><subject>Bone Morphogenetic Protein 4 - pharmacology</subject><subject>cell</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Line</subject><subject>cells</subject><subject>DNA Methylation - genetics</subject><subject>Embryonic</subject><subject>Embryonic stem cells</subject><subject>Embryonic Stem Cells - physiology</subject><subject>factor</subject><subject>Humans</subject><subject>Methylation</subject><subject>Rats</subject><subject>stem</subject><subject>Thromboplastin - genetics</subject><subject>Thromboplastin - metabolism</subject><subject>Tissue</subject><subject>Tissue factor</subject><subject>Trophoblast</subject><subject>Trophoblast cell</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - physiology</subject><issn>1995-7645</issn><issn>2352-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhi0EoqvSnwCKxKUgBfwVJz4hVPElVeJAOVuOM951tbG3tlNIfz1Os5RjsSXbYz0z82pehF4S_I5gIt7_IFI2dSt4c074G4GxZLV4gjaUNbTmhIunaPOAnKCzlK5xWYxK2bLn6IQ2WDDaiQ0KVy6lCSqrTQ6xgt-HCCm54Cvth2qEvJv3Oi9xhO10fDpfDc5aiOCzW7-CrWDs4xy8M1XKMFYG9vtU0ByqHMNhF_q9TvkFemb1PsHZ8T5FPz9_urr4Wl9-__Lt4uNlbRpOci0Fa3sQJRDUSs56MJT2RhosRMuwwUNviWxYJznRZfedZRSWg3fGdoSdordr3V_aW-236jpM0ZeOao7DPN_dKaCYcNxiggt8vsKHGG4mSFmNLi36tYcwJUWEwEy2GDf_gVIqMOVF5aNowztRytJFQLOiJoaUIlh1iG7UcVYEq8Vxde-4WuxUhKt7x5Uoea-OLaZ-hOEh66-_BfiwAlBmfesgqmQceAODi2CyGoJ7tMXro7Rd8NsbV0b5TxtmmImuTPAPi1jG_g</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Liu, Lin-Xin</creator><creator>Zeng, Hui</creator><creator>Liu, En-Yi</creator><creator>Chen, Fang-Ping</creator><general>Elsevier B.V</general><general>Department of Hematology, Xiangya Hospital of Central South University, Changsha 410008, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20140701</creationdate><title>Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast</title><author>Liu, Lin-Xin ; Zeng, Hui ; Liu, En-Yi ; Chen, Fang-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-9637be6c5462f943bec22bc9c066730c0dbf19538941a1a1b8f32e8f3248cf813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Bone Morphogenetic Protein 4 - pharmacology</topic><topic>cell</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Line</topic><topic>cells</topic><topic>DNA Methylation - genetics</topic><topic>Embryonic</topic><topic>Embryonic stem cells</topic><topic>Embryonic Stem Cells - physiology</topic><topic>factor</topic><topic>Humans</topic><topic>Methylation</topic><topic>Rats</topic><topic>stem</topic><topic>Thromboplastin - genetics</topic><topic>Thromboplastin - metabolism</topic><topic>Tissue</topic><topic>Tissue factor</topic><topic>Trophoblast</topic><topic>Trophoblast cell</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Lin-Xin</creatorcontrib><creatorcontrib>Zeng, Hui</creatorcontrib><creatorcontrib>Liu, En-Yi</creatorcontrib><creatorcontrib>Chen, Fang-Ping</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Asian Pacific journal of tropical medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Lin-Xin</au><au>Zeng, Hui</au><au>Liu, En-Yi</au><au>Chen, Fang-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast</atitle><jtitle>Asian Pacific journal of tropical medicine</jtitle><addtitle>Asian Pacific Journal of Tropical Medicine</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>7</volume><issue>7</issue><spage>557</spage><epage>561</epage><pages>557-561</pages><issn>1995-7645</issn><eissn>2352-4146</eissn><abstract>Objective:To explore tissue factor(TF)expression and methylation regulation in differentiation of human embryonic stem cells(hESCs)into trophoblast.Methods:Differentiation of hESCs into trophoblast was induced by bone morphogenetic protein 4(BMP4).Expression of gene,protein of TF and DNA methylation at different time points during induction process was detected by RTPCT,Western blot,flow cytometry and MSP-PCR method.Results:The expression of mRNA,protein level of TF could be detected during directional differentiation of hESCs to trophoblast cells,semi methylation-semi non methylation expression appeared at TF DNA promoter region,and it showed decreased methylation level and increased non methylation level with formation of trophoblast cell and increased expression of TF.Conclusions:It shows that during differentiation of hESCs into trophoblast,the differential expression of TF is related with DNA methylation level,and it is changed with the methylation or non methylated degree.It provids new platform to furtherly explore the regulation mechanisms of specific expression of tissue factor in the process of the embryonic stem cell development.</abstract><cop>India</cop><pub>Elsevier B.V</pub><pmid>25063286</pmid><doi>10.1016/S1995-7645(14)60093-6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bone Morphogenetic Protein 4 - pharmacology cell Cell Differentiation - drug effects Cell Differentiation - genetics Cell Line cells DNA Methylation - genetics Embryonic Embryonic stem cells Embryonic Stem Cells - physiology factor Humans Methylation Rats stem Thromboplastin - genetics Thromboplastin - metabolism Tissue Tissue factor Trophoblast Trophoblast cell Trophoblasts - metabolism Trophoblasts - physiology |
| title | Tissue factor expression and methylation regulation in differentiation of embryonic stem cells into trophoblast |
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