Molecular Mechanism of Bovine Trabecular Meshwork Cells Apoptosis Induced by Dexamethasone and Protection by Pilocarpine
Purpose: To study the molecular mechanism of trabecular meshwork cells apoptosis induced by dexamethasone and the protection of pilocarpine.Methods: Determining mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR), protein expression with Western blots and the percentage of...
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| Veröffentlicht in: | Yanke xuebao 2005-03, Vol.21 (1), p.44-51 |
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| creator | Gu, Yajuan Zeng, Shujun Qiu, Pengxin Wu, Yuping Peng, Dawei Yan, Guangmei |
| description | Purpose: To study the molecular mechanism of trabecular meshwork cells apoptosis induced by dexamethasone and the protection of pilocarpine.Methods: Determining mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR), protein expression with Western blots and the percentage of apoptotic cells with fluorescent microscopy.Results: Dexamethasone up-regulated Fas proteins and affected Bax, caspase-8 and caspase-9 proteins in an action of first decrease then increase. Pre-treatment with pilocarpine decreased the four proteins expression, which were increased by dexamethasone. Pilocarpine self could decrease pro-apoptotlc factors Bax, caspase-8 and caspase-9 proteins expression.Conclusion: Fas/FasL pathway participated in apoptotic process induced by dexamethasone in trabecular meshwork cells and the process was probably related with both caspase-8 and caspase-9 pathways. Pilocarpine protected the cells against apoptosis through down-regulating Fas, Bax, caspase-8 and caspase-9 proteins expression. Eye Science 2005;21:44-51. |
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Pre-treatment with pilocarpine decreased the four proteins expression, which were increased by dexamethasone. Pilocarpine self could decrease pro-apoptotlc factors Bax, caspase-8 and caspase-9 proteins expression.Conclusion: Fas/FasL pathway participated in apoptotic process induced by dexamethasone in trabecular meshwork cells and the process was probably related with both caspase-8 and caspase-9 pathways. Pilocarpine protected the cells against apoptosis through down-regulating Fas, Bax, caspase-8 and caspase-9 proteins expression. Eye Science 2005;21:44-51.</description><identifier>ISSN: 1000-4432</identifier><identifier>PMID: 17162916</identifier><language>eng</language><publisher>China: Department of Pharmacology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China%Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China</publisher><subject>Animals ; Apoptosis - drug effects ; bcl-2-Associated X Protein - genetics ; bcl-2-Associated X Protein - metabolism ; Caspase 8 - genetics ; Caspase 8 - metabolism ; Caspase 9 - genetics ; Caspase 9 - metabolism ; Cattle ; Cells, Cultured ; Dexamethasone - pharmacology ; Fas Ligand Protein - genetics ; Fas Ligand Protein - metabolism ; fas Receptor - genetics ; fas Receptor - metabolism ; Pilocarpine - pharmacology ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal Transduction - drug effects ; Trabecular Meshwork - metabolism ; Trabecular Meshwork - pathology ; 分子机制 ; 地塞米松 ; 基因表达 ; 小梁细胞 ; 毛果芸香碱 ; 细胞调亡 ; 药物诱导</subject><ispartof>Yanke xuebao, 2005-03, Vol.21 (1), p.44-51</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/98174A/98174A.jpg</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17162916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Yajuan</creatorcontrib><creatorcontrib>Zeng, Shujun</creatorcontrib><creatorcontrib>Qiu, Pengxin</creatorcontrib><creatorcontrib>Wu, Yuping</creatorcontrib><creatorcontrib>Peng, Dawei</creatorcontrib><creatorcontrib>Yan, Guangmei</creatorcontrib><title>Molecular Mechanism of Bovine Trabecular Meshwork Cells Apoptosis Induced by Dexamethasone and Protection by Pilocarpine</title><title>Yanke xuebao</title><addtitle>Eye Science</addtitle><description>Purpose: To study the molecular mechanism of trabecular meshwork cells apoptosis induced by dexamethasone and the protection of pilocarpine.Methods: Determining mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR), protein expression with Western blots and the percentage of apoptotic cells with fluorescent microscopy.Results: Dexamethasone up-regulated Fas proteins and affected Bax, caspase-8 and caspase-9 proteins in an action of first decrease then increase. Pre-treatment with pilocarpine decreased the four proteins expression, which were increased by dexamethasone. Pilocarpine self could decrease pro-apoptotlc factors Bax, caspase-8 and caspase-9 proteins expression.Conclusion: Fas/FasL pathway participated in apoptotic process induced by dexamethasone in trabecular meshwork cells and the process was probably related with both caspase-8 and caspase-9 pathways. Pilocarpine protected the cells against apoptosis through down-regulating Fas, Bax, caspase-8 and caspase-9 proteins expression. Eye Science 2005;21:44-51.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Caspase 8 - genetics</subject><subject>Caspase 8 - metabolism</subject><subject>Caspase 9 - genetics</subject><subject>Caspase 9 - metabolism</subject><subject>Cattle</subject><subject>Cells, Cultured</subject><subject>Dexamethasone - pharmacology</subject><subject>Fas Ligand Protein - genetics</subject><subject>Fas Ligand Protein - metabolism</subject><subject>fas Receptor - genetics</subject><subject>fas Receptor - metabolism</subject><subject>Pilocarpine - pharmacology</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Trabecular Meshwork - metabolism</subject><subject>Trabecular Meshwork - pathology</subject><subject>分子机制</subject><subject>地塞米松</subject><subject>基因表达</subject><subject>小梁细胞</subject><subject>毛果芸香碱</subject><subject>细胞调亡</subject><subject>药物诱导</subject><issn>1000-4432</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtPAjEUhWehEUT_gmlMjCuSPubVJeKLBCILXE_udFoozLRDOyPw761BXd3F-c45ueciGhKM8TiOGR1E195vMY4pTvOraEAyklJO0mF0XNhair4GhxZSbMBo3yCr0JP90kailYPyX_abg3U7NJV17dGktW1nvfZoZqpeyAqVJ_Qsj9DIbgPeBjOYCi2d7aTotDU_-lLXVoBrQ_RNdKmg9vL2946iz9eX1fR9PP94m00n87EgOeXjTPGU45grRjLKsGCqwrIEThOGc0oVqJwnpZIJF5ATphSGSoiEE8xonsQZG0UP59wDGAVmXWxt70xoLE67Y0kxTnCYiQfu8cy1zu576bui0V6EV8FI2_siYzHJ8pjHgbz7JfuykVXROt2AOxV_owbg_gyIjTXrvQ6lJYid0rUsSEISmuaMfQOANH3P</recordid><startdate>200503</startdate><enddate>200503</enddate><creator>Gu, Yajuan</creator><creator>Zeng, Shujun</creator><creator>Qiu, Pengxin</creator><creator>Wu, Yuping</creator><creator>Peng, Dawei</creator><creator>Yan, Guangmei</creator><general>Department of Pharmacology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China%Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>200503</creationdate><title>Molecular Mechanism of Bovine Trabecular Meshwork Cells Apoptosis Induced by Dexamethasone and Protection by Pilocarpine</title><author>Gu, Yajuan ; Zeng, Shujun ; Qiu, Pengxin ; Wu, Yuping ; Peng, Dawei ; Yan, Guangmei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1829-7f969049f317230c3fd0eba92530822faf895bfe59ca813ff0adcc59103285473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Caspase 8 - genetics</topic><topic>Caspase 8 - metabolism</topic><topic>Caspase 9 - genetics</topic><topic>Caspase 9 - metabolism</topic><topic>Cattle</topic><topic>Cells, Cultured</topic><topic>Dexamethasone - pharmacology</topic><topic>Fas Ligand Protein - genetics</topic><topic>Fas Ligand Protein - metabolism</topic><topic>fas Receptor - genetics</topic><topic>fas Receptor - metabolism</topic><topic>Pilocarpine - pharmacology</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Trabecular Meshwork - metabolism</topic><topic>Trabecular Meshwork - pathology</topic><topic>分子机制</topic><topic>地塞米松</topic><topic>基因表达</topic><topic>小梁细胞</topic><topic>毛果芸香碱</topic><topic>细胞调亡</topic><topic>药物诱导</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Yajuan</creatorcontrib><creatorcontrib>Zeng, Shujun</creatorcontrib><creatorcontrib>Qiu, Pengxin</creatorcontrib><creatorcontrib>Wu, Yuping</creatorcontrib><creatorcontrib>Peng, Dawei</creatorcontrib><creatorcontrib>Yan, Guangmei</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Yanke xuebao</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Yajuan</au><au>Zeng, Shujun</au><au>Qiu, Pengxin</au><au>Wu, Yuping</au><au>Peng, Dawei</au><au>Yan, Guangmei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Mechanism of Bovine Trabecular Meshwork Cells Apoptosis Induced by Dexamethasone and Protection by Pilocarpine</atitle><jtitle>Yanke xuebao</jtitle><addtitle>Eye Science</addtitle><date>2005-03</date><risdate>2005</risdate><volume>21</volume><issue>1</issue><spage>44</spage><epage>51</epage><pages>44-51</pages><issn>1000-4432</issn><abstract>Purpose: To study the molecular mechanism of trabecular meshwork cells apoptosis induced by dexamethasone and the protection of pilocarpine.Methods: Determining mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR), protein expression with Western blots and the percentage of apoptotic cells with fluorescent microscopy.Results: Dexamethasone up-regulated Fas proteins and affected Bax, caspase-8 and caspase-9 proteins in an action of first decrease then increase. Pre-treatment with pilocarpine decreased the four proteins expression, which were increased by dexamethasone. Pilocarpine self could decrease pro-apoptotlc factors Bax, caspase-8 and caspase-9 proteins expression.Conclusion: Fas/FasL pathway participated in apoptotic process induced by dexamethasone in trabecular meshwork cells and the process was probably related with both caspase-8 and caspase-9 pathways. Pilocarpine protected the cells against apoptosis through down-regulating Fas, Bax, caspase-8 and caspase-9 proteins expression. Eye Science 2005;21:44-51.</abstract><cop>China</cop><pub>Department of Pharmacology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China%Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China</pub><pmid>17162916</pmid><tpages>8</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects bcl-2-Associated X Protein - genetics bcl-2-Associated X Protein - metabolism Caspase 8 - genetics Caspase 8 - metabolism Caspase 9 - genetics Caspase 9 - metabolism Cattle Cells, Cultured Dexamethasone - pharmacology Fas Ligand Protein - genetics Fas Ligand Protein - metabolism fas Receptor - genetics fas Receptor - metabolism Pilocarpine - pharmacology RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction - drug effects Trabecular Meshwork - metabolism Trabecular Meshwork - pathology 分子机制 地塞米松 基因表达 小梁细胞 毛果芸香碱 细胞调亡 药物诱导 |
| title | Molecular Mechanism of Bovine Trabecular Meshwork Cells Apoptosis Induced by Dexamethasone and Protection by Pilocarpine |
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