蕲蛇蛇毒中一种新型金属蛋白酶AA-MP-I的纯化和表征
本研究通过嗜硫色谱、Sephadex G-75、蓝胶和POROS HQ20离子交换色谱,从蕲蛇蛇毒中分离得到一种新组分AA-MP-I。该酶为分子量22.9kDa的单体蛋白,等电点为5.55,不含中性糖基,N端序列为STE-FQRYMEIVIVVDHSMVK,结果表明其为新型P-I型金属蛋白酶,对温度敏感,具有抗凝血活性,40℃下抗凝血活性最强,具有出血毒性,无磷脂酶A2活性。...
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| Veröffentlicht in: | Tian ran chan wu yan jiu yu kai fa 2010, Vol.22 (6), p.929-933 |
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| container_title | Tian ran chan wu yan jiu yu kai fa |
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| creator | 潘剑茹 何火聪 刘枋 饶平凡 |
| description | 本研究通过嗜硫色谱、Sephadex G-75、蓝胶和POROS HQ20离子交换色谱,从蕲蛇蛇毒中分离得到一种新组分AA-MP-I。该酶为分子量22.9kDa的单体蛋白,等电点为5.55,不含中性糖基,N端序列为STE-FQRYMEIVIVVDHSMVK,结果表明其为新型P-I型金属蛋白酶,对温度敏感,具有抗凝血活性,40℃下抗凝血活性最强,具有出血毒性,无磷脂酶A2活性。 |
| doi_str_mv | 10.3969/j.issn.1001-6880.2010.06.001 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1001-6880 |
| ispartof | Tian ran chan wu yan jiu yu kai fa, 2010, Vol.22 (6), p.929-933 |
| issn | 1001-6880 |
| language | chi |
| recordid | cdi_wanfang_journals_trcwyjykf201006001 |
| source | Business Source Complete |
| subjects | 出血毒 抗凝血 蕲蛇 蛇毒 金属蛋白酶 |
| title | 蕲蛇蛇毒中一种新型金属蛋白酶AA-MP-I的纯化和表征 |
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