Hepcidin and Iron Metabolism in Non-diabetic Obese and Type 2 Diabetic Rats
Summary： The aim of this study was to investigate the changes of iron levels and hepatic regulatory molecules expression involved in iron metabolism in non-diabetic obese/type 2 diabetic rat models. Male Wistar rats were divided into 3 groups： control group, non-diabetic obese group and type 2 dia-...
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| creator | Chen, Yue Yin, Hui-qing Liu, Hao-ling Xiu, Lei Peng, Xiao-yu |
| description | Summary： The aim of this study was to investigate the changes of iron levels and hepatic regulatory molecules expression involved in iron metabolism in non-diabetic obese/type 2 diabetic rat models. Male Wistar rats were divided into 3 groups： control group, non-diabetic obese group and type 2 dia- betic group （n=20 each）. The rats were evaluated physiologically and biochemically. The hepatic histo- pathological changes were observed using haematoxylin and eosin （HE） staining. The mRNA expres- sion patterns of hepcidin, interleukin-6 （IL-6）, hypoxia-inducible factor （HIF） and ferroportin （Fpn） in the rat liver in control group, non-diabetic obese group and type 2 diabetic group were analyzed by real-time RT-PCR. The protein expression patterns of hepcidin in liver of each group were further ana- lyzed by immunohistochemistry and Western blotting. As compared with control group, the ferritin in non-diabetic obese group and type 2 diabetic group was increased significantly （P〈0.001）. However, there was no significant difference in soluble transferring receptor （sTfR）：ferritin ratio among the three groups （P〉0.05）. The real-time RT-PCR, immunohistochemistry and Western blotting results all re- vealed that the expression levels of hepcidin in non-diabetic obese group and type 2 diabetic group were elevated significantly as compared with those in control group （P〈0.001）. The expression levels of hep- cidin mRNA between non-diabetic obese group and type 2 diabetic group showed no significant differ- ence （P〉0.05）. However, the protein expression levels of hepcidin in type 2 diabetic group were sig- nificantly higher than those in non-diabetic obese group （P〈0.05）. Compared to control group, the ex- pression levels of IL-6 mRNA in non-diabetic obese group and type 2 diabetic group were increased significantly and the expression levels ofFpn mRNA decreased （P〈0.05）. However, the expression lev- els ofHIF mRNA had no significant difference among three groups. It is suggested that iron metabolism is substantially disturbed in non-diabetic obese and type 2 diabetic rats probably by the abnormal ex- pression of hepcidin in chronic inflammatory status. The increased hepcidin may restrain the iron re- lease from the cells by affecting the expression of Fpn, which probably associates with the development of diabetic complication. |
| doi_str_mv | 10.1007/s11596-015-1517-z |
| format | Article |
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Male Wistar rats were divided into 3 groups： control group, non-diabetic obese group and type 2 dia- betic group （n=20 each）. The rats were evaluated physiologically and biochemically. The hepatic histo- pathological changes were observed using haematoxylin and eosin （HE） staining. The mRNA expres- sion patterns of hepcidin, interleukin-6 （IL-6）, hypoxia-inducible factor （HIF） and ferroportin （Fpn） in the rat liver in control group, non-diabetic obese group and type 2 diabetic group were analyzed by real-time RT-PCR. The protein expression patterns of hepcidin in liver of each group were further ana- lyzed by immunohistochemistry and Western blotting. As compared with control group, the ferritin in non-diabetic obese group and type 2 diabetic group was increased significantly （P〈0.001）. However, there was no significant difference in soluble transferring receptor （sTfR）：ferritin ratio among the three groups （P〉0.05）. The real-time RT-PCR, immunohistochemistry and Western blotting results all re- vealed that the expression levels of hepcidin in non-diabetic obese group and type 2 diabetic group were elevated significantly as compared with those in control group （P〈0.001）. The expression levels of hep- cidin mRNA between non-diabetic obese group and type 2 diabetic group showed no significant differ- ence （P〉0.05）. However, the protein expression levels of hepcidin in type 2 diabetic group were sig- nificantly higher than those in non-diabetic obese group （P〈0.05）. Compared to control group, the ex- pression levels of IL-6 mRNA in non-diabetic obese group and type 2 diabetic group were increased significantly and the expression levels ofFpn mRNA decreased （P〈0.05）. However, the expression lev- els ofHIF mRNA had no significant difference among three groups. It is suggested that iron metabolism is substantially disturbed in non-diabetic obese and type 2 diabetic rats probably by the abnormal ex- pression of hepcidin in chronic inflammatory status. The increased hepcidin may restrain the iron re- lease from the cells by affecting the expression of Fpn, which probably associates with the development of diabetic complication.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-015-1517-z</identifier><identifier>PMID: 26670435</identifier><language>eng</language><publisher>Wuhan: Huazhong University of Science and Technology</publisher><subject>2型糖尿病 ; Animals ; Diabetes Mellitus, Type 2 - complications ; Diabetes Mellitus, Type 2 - metabolism ; Hepcidin ; Hepcidins - genetics ; Hepcidins - metabolism ; Interleukin-6 - genetics ; Iron - metabolism ; Male ; Medicine ; Medicine & Public Health ; mRNA表达 ; Obesity - complications ; Obesity - metabolism ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - genetics ; 实时定量RT-PCR ; 白细胞介素-6 ; 糖尿病大鼠 ; 肥胖 ; 铁代谢</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2015-12, Vol.35 (6), p.851-857</ispartof><rights>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2015</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-36a70abba1c64669a9abfe44f64018865d30091a1d0a2858eab67eae8590c5223</citedby><cites>FETCH-LOGICAL-c407t-36a70abba1c64669a9abfe44f64018865d30091a1d0a2858eab67eae8590c5223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26670435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yue</creatorcontrib><creatorcontrib>Yin, Hui-qing</creatorcontrib><creatorcontrib>Liu, Hao-ling</creatorcontrib><creatorcontrib>Xiu, Lei</creatorcontrib><creatorcontrib>Peng, Xiao-yu</creatorcontrib><title>Hepcidin and Iron Metabolism in Non-diabetic Obese and Type 2 Diabetic Rats</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</addtitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><description>Summary： The aim of this study was to investigate the changes of iron levels and hepatic regulatory molecules expression involved in iron metabolism in non-diabetic obese/type 2 diabetic rat models. Male Wistar rats were divided into 3 groups： control group, non-diabetic obese group and type 2 dia- betic group （n=20 each）. The rats were evaluated physiologically and biochemically. The hepatic histo- pathological changes were observed using haematoxylin and eosin （HE） staining. The mRNA expres- sion patterns of hepcidin, interleukin-6 （IL-6）, hypoxia-inducible factor （HIF） and ferroportin （Fpn） in the rat liver in control group, non-diabetic obese group and type 2 diabetic group were analyzed by real-time RT-PCR. The protein expression patterns of hepcidin in liver of each group were further ana- lyzed by immunohistochemistry and Western blotting. As compared with control group, the ferritin in non-diabetic obese group and type 2 diabetic group was increased significantly （P〈0.001）. However, there was no significant difference in soluble transferring receptor （sTfR）：ferritin ratio among the three groups （P〉0.05）. The real-time RT-PCR, immunohistochemistry and Western blotting results all re- vealed that the expression levels of hepcidin in non-diabetic obese group and type 2 diabetic group were elevated significantly as compared with those in control group （P〈0.001）. The expression levels of hep- cidin mRNA between non-diabetic obese group and type 2 diabetic group showed no significant differ- ence （P〉0.05）. However, the protein expression levels of hepcidin in type 2 diabetic group were sig- nificantly higher than those in non-diabetic obese group （P〈0.05）. Compared to control group, the ex- pression levels of IL-6 mRNA in non-diabetic obese group and type 2 diabetic group were increased significantly and the expression levels ofFpn mRNA decreased （P〈0.05）. However, the expression lev- els ofHIF mRNA had no significant difference among three groups. It is suggested that iron metabolism is substantially disturbed in non-diabetic obese and type 2 diabetic rats probably by the abnormal ex- pression of hepcidin in chronic inflammatory status. The increased hepcidin may restrain the iron re- lease from the cells by affecting the expression of Fpn, which probably associates with the development of diabetic complication.</description><subject>2型糖尿病</subject><subject>Animals</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Hepcidin</subject><subject>Hepcidins - genetics</subject><subject>Hepcidins - metabolism</subject><subject>Interleukin-6 - genetics</subject><subject>Iron - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>mRNA表达</subject><subject>Obesity - complications</subject><subject>Obesity - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>实时定量RT-PCR</subject><subject>白细胞介素-6</subject><subject>糖尿病大鼠</subject><subject>肥胖</subject><subject>铁代谢</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0Eoh_wA7igiBNSZZixYzs5ohZoRaESKmdrnEyWLLvONs6Kbn89LtmWGydbnud9PHqFeIXwDgHc-4RoaisBjUSDTt49EYdY11qiNuppvlunJDitD8RRSksA46wqn4sDZa2DUptD8eWcN03f9rGg2BYX4xCLrzxRGFZ9Whf5-dsQZdtT4KlviqvAif-S17sNF6o4e5h8pym9EM86WiV-uT-PxY9PH69Pz-Xl1eeL0w-XsinBTVJbckAhEDa2tLammkLHZdnZErCqrGk1QI2ELZCqTMUUrGPiytTQGKX0sTiZvb8pdhQXfjlsx5h_9NNy96u9vQ2eVS4FLCBk-u1Mb8bhZstp8us-NbxaUeRhmzw6AwCICjOKM9qMQ0ojd34z9msadx7B3zfu58Z9lvv7xv1dzrze67dhze1j4qHiDKgZSHkUFzz-W_d_1jf7TX4OcXGTc4_i7EWrqrLWfwD-J5Ur</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Chen, Yue</creator><creator>Yin, Hui-qing</creator><creator>Liu, Hao-ling</creator><creator>Xiu, Lei</creator><creator>Peng, Xiao-yu</creator><general>Huazhong University of Science and Technology</general><general>Department of Endocrinology, the First Affiliated Hospital of Harbin Medical University, Harbin 150001, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20151201</creationdate><title>Hepcidin and Iron Metabolism in Non-diabetic Obese and Type 2 Diabetic Rats</title><author>Chen, Yue ; Yin, Hui-qing ; Liu, Hao-ling ; Xiu, Lei ; Peng, Xiao-yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-36a70abba1c64669a9abfe44f64018865d30091a1d0a2858eab67eae8590c5223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>2型糖尿病</topic><topic>Animals</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Hepcidin</topic><topic>Hepcidins - genetics</topic><topic>Hepcidins - metabolism</topic><topic>Interleukin-6 - genetics</topic><topic>Iron - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>mRNA表达</topic><topic>Obesity - complications</topic><topic>Obesity - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>实时定量RT-PCR</topic><topic>白细胞介素-6</topic><topic>糖尿病大鼠</topic><topic>肥胖</topic><topic>铁代谢</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yue</creatorcontrib><creatorcontrib>Yin, Hui-qing</creatorcontrib><creatorcontrib>Liu, Hao-ling</creatorcontrib><creatorcontrib>Xiu, Lei</creatorcontrib><creatorcontrib>Peng, Xiao-yu</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yue</au><au>Yin, Hui-qing</au><au>Liu, Hao-ling</au><au>Xiu, Lei</au><au>Peng, Xiao-yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepcidin and Iron Metabolism in Non-diabetic Obese and Type 2 Diabetic Rats</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><stitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</stitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><date>2015-12-01</date><risdate>2015</risdate><volume>35</volume><issue>6</issue><spage>851</spage><epage>857</epage><pages>851-857</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Summary： The aim of this study was to investigate the changes of iron levels and hepatic regulatory molecules expression involved in iron metabolism in non-diabetic obese/type 2 diabetic rat models. Male Wistar rats were divided into 3 groups： control group, non-diabetic obese group and type 2 dia- betic group （n=20 each）. The rats were evaluated physiologically and biochemically. The hepatic histo- pathological changes were observed using haematoxylin and eosin （HE） staining. The mRNA expres- sion patterns of hepcidin, interleukin-6 （IL-6）, hypoxia-inducible factor （HIF） and ferroportin （Fpn） in the rat liver in control group, non-diabetic obese group and type 2 diabetic group were analyzed by real-time RT-PCR. The protein expression patterns of hepcidin in liver of each group were further ana- lyzed by immunohistochemistry and Western blotting. As compared with control group, the ferritin in non-diabetic obese group and type 2 diabetic group was increased significantly （P〈0.001）. However, there was no significant difference in soluble transferring receptor （sTfR）：ferritin ratio among the three groups （P〉0.05）. The real-time RT-PCR, immunohistochemistry and Western blotting results all re- vealed that the expression levels of hepcidin in non-diabetic obese group and type 2 diabetic group were elevated significantly as compared with those in control group （P〈0.001）. The expression levels of hep- cidin mRNA between non-diabetic obese group and type 2 diabetic group showed no significant differ- ence （P〉0.05）. However, the protein expression levels of hepcidin in type 2 diabetic group were sig- nificantly higher than those in non-diabetic obese group （P〈0.05）. Compared to control group, the ex- pression levels of IL-6 mRNA in non-diabetic obese group and type 2 diabetic group were increased significantly and the expression levels ofFpn mRNA decreased （P〈0.05）. However, the expression lev- els ofHIF mRNA had no significant difference among three groups. It is suggested that iron metabolism is substantially disturbed in non-diabetic obese and type 2 diabetic rats probably by the abnormal ex- pression of hepcidin in chronic inflammatory status. The increased hepcidin may restrain the iron re- lease from the cells by affecting the expression of Fpn, which probably associates with the development of diabetic complication.</abstract><cop>Wuhan</cop><pub>Huazhong University of Science and Technology</pub><pmid>26670435</pmid><doi>10.1007/s11596-015-1517-z</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2015-12, Vol.35 (6), p.851-857 |
| issn | 1672-0733 1993-1352 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 2型糖尿病 Animals Diabetes Mellitus, Type 2 - complications Diabetes Mellitus, Type 2 - metabolism Hepcidin Hepcidins - genetics Hepcidins - metabolism Interleukin-6 - genetics Iron - metabolism Male Medicine Medicine & Public Health mRNA表达 Obesity - complications Obesity - metabolism Rats Rats, Wistar Real-Time Polymerase Chain Reaction RNA, Messenger - genetics 实时定量RT-PCR 白细胞介素-6 糖尿病大鼠 肥胖 铁代谢 |
| title | Hepcidin and Iron Metabolism in Non-diabetic Obese and Type 2 Diabetic Rats |
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