Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury
The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2014-08, Vol.34 (4), p.548-553 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良 |
| description | The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice. |
| doi_str_mv | 10.1007/s11596-014-1313-1 |
| format | Article |
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Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-014-1313-1</identifier><identifier>PMID: 25135725</identifier><language>eng</language><publisher>Heidelberg: Huazhong University of Science and Technology</publisher><subject>Animals ; Biomarkers - metabolism ; Brain Injuries - metabolism ; Brain Injuries - pathology ; Female ; Forensic Genetics ; Gene Expression Profiling ; Gene Expression Regulation ; Hippocampus - metabolism ; Hippocampus - pathology ; Male ; Medicine ; Medicine & Public Health ; microRNA ; MicroRNAs - biosynthesis ; Rats ; Rats, Sprague-Dawley ; SD大鼠 ; 创伤性脑损伤 ; 微RNA ; 微小RNA ; 微阵列分析 ; 海马 ; 表达谱</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2014-08, Vol.34 (4), p.548-553</ispartof><rights>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-f265e63c860922d2114b9e863fc3537857dbc6cbcb6610ac96a4ce36001c33813</citedby><cites>FETCH-LOGICAL-c407t-f265e63c860922d2114b9e863fc3537857dbc6cbcb6610ac96a4ce36001c33813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25135725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良</creatorcontrib><title>Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</addtitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><description>The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. MiR-142-3p and miR-221 may be used as potentially biological markers for TBI assessment in forensic practice.</description><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Brain Injuries - metabolism</subject><subject>Brain Injuries - pathology</subject><subject>Female</subject><subject>Forensic Genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>microRNA</subject><subject>MicroRNAs - biosynthesis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>SD大鼠</subject><subject>创伤性脑损伤</subject><subject>微RNA</subject><subject>微小RNA</subject><subject>微阵列分析</subject><subject>海马</subject><subject>表达谱</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAURiMEoi9-ABsUsUKqAvf6lWRZqr6k8lDVLlhZjscZPCR2aifqzL-vowxlx8pXuud-_nSy7D3CZwQov0REXosCkBVIkRb4KjvEuk4D5eR1mkVJCigpPciOYtwA8FIQ9jY7IDwRJeGH2a-L7RBMjNa7_Gfwre2sW-e-zb9ZHfzd97OYW5df22HwWvXDFOfdnRpjfum7zj_N9H1QU69Gq_OvQSX6xm2msDvJ3rSqi-bd_j3OHi4v7s-vi9sfVzfnZ7eFZlCORUsEN4LqSkBNyIogsqY2laCtppyWFS9XjRa60Y0QCErXQjFtqABATWmF9Dg7XXKflGuVW8uNn4JLP8pxs_uz2m4baUhSBAyQJvrTQg_BP04mjrK3UZuuU874KUrknNUMkUBCcUGTiBiDaeUQbK_CTiLI2b9c_MsULmf_ci7zYR8_Nb1ZvVz8FZ4AsgAxrdzahH91_5f6cd_kt3frx3T3EiwEASCMVPQZ37qadA</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良</creator><general>Huazhong University of Science and Technology</general><general>Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Forensic Expertise Centre of Xiling District Public Security Bureau, Yichang 443001, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20140801</creationdate><title>Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury</title><author>孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-f265e63c860922d2114b9e863fc3537857dbc6cbcb6610ac96a4ce36001c33813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Biomarkers - metabolism</topic><topic>Brain Injuries - metabolism</topic><topic>Brain Injuries - pathology</topic><topic>Female</topic><topic>Forensic Genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - pathology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>microRNA</topic><topic>MicroRNAs - biosynthesis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>SD大鼠</topic><topic>创伤性脑损伤</topic><topic>微RNA</topic><topic>微小RNA</topic><topic>微阵列分析</topic><topic>海马</topic><topic>表达谱</topic><toplevel>online_resources</toplevel><creatorcontrib>孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>孙婷怡 陈晓瑞 刘子龙 赵丽丽 江永祥 屈国强 王荣帅 黄锶哲 刘良</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><stitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</stitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>34</volume><issue>4</issue><spage>548</spage><epage>553</epage><pages>548-553</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>The changes of microRNA expression in rat hippocampus after traumatic brain injury（TBI） were explored. Adult SD rats received a single controlled cortical impact injury, and the ipsilateral hippocampus was harvested for the subsequent microarray assay at three time points after TBI： 1st day, 3rd day and 5th day, respectively. We characterized the microRNA expression profile in rat hippocampus using the microRNA microarray analysis, and further verified microarray results of miR-142-3p and miR-221 using quantitative real-time PCR. Totally 205 microRNAs were identified and up-/down-regu-lated more than 1.5 times. There were significant changes in 17 microRNAs at all three time points post-TBI. The quantitative real-time PCR results of miR-142-3p and miR-221 indicated good consistency with the results of the microarray method. MicroRNAs altered at different time points post-TBI. 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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2014-08, Vol.34 (4), p.548-553 |
| issn | 1672-0733 1993-1352 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biomarkers - metabolism Brain Injuries - metabolism Brain Injuries - pathology Female Forensic Genetics Gene Expression Profiling Gene Expression Regulation Hippocampus - metabolism Hippocampus - pathology Male Medicine Medicine & Public Health microRNA MicroRNAs - biosynthesis Rats Rats, Sprague-Dawley SD大鼠 创伤性脑损伤 微RNA 微小RNA 微阵列分析 海马 表达谱 |
| title | Expression Profiling of MicroRNAs in Hippocampus of Rats Following Traumatic Brain Injury |
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