Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family
Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineo...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2014-02, Vol.34 (1), p.125-130 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 陈静 李辉敏 张学农 熊朝梅 阮金兰 |
| description | Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family. |
| doi_str_mv | 10.1007/s11596-014-1243-y |
| format | Article |
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In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-014-1243-y</identifier><identifier>PMID: 24496691</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Apoptosis - drug effects ; Bax ; Bcl-2 ; bcl-2-Associated X Protein - metabolism ; Bcl-2蛋白 ; Blotting, Western ; Caspase 3 - metabolism ; Caspase-3 ; Cell Line, Tumor ; Cell Survival - drug effects ; Diosgenin - analogs & derivatives ; Diosgenin - chemistry ; Diosgenin - pharmacology ; Dose-Response Relationship, Drug ; Enzyme Activation - drug effects ; Flow Cytometry ; Humans ; LNCaP细胞 ; Male ; Medicine ; Medicine & Public Health ; Molecular Structure ; Prostatic Neoplasms - metabolism ; Prostatic Neoplasms - pathology ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Time Factors ; 前列腺癌细胞 ; 细胞凋亡 ; 薯蓣皂甙</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2014-02, Vol.34 (1), p.125-130</ispartof><rights>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2014</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-89993168cd3ec150cdefce1584e068d656d270eb7a6795ed1ca0007d26ca8d293</citedby><cites>FETCH-LOGICAL-c406t-89993168cd3ec150cdefce1584e068d656d270eb7a6795ed1ca0007d26ca8d293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24496691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>陈静 李辉敏 张学农 熊朝梅 阮金兰</creatorcontrib><title>Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</addtitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><description>Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.</description><subject>Apoptosis - drug effects</subject><subject>Bax</subject><subject>Bcl-2</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Bcl-2蛋白</subject><subject>Blotting, Western</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase-3</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Diosgenin - analogs & derivatives</subject><subject>Diosgenin - chemistry</subject><subject>Diosgenin - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enzyme Activation - drug effects</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>LNCaP细胞</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Molecular Structure</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Prostatic Neoplasms - pathology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Time Factors</subject><subject>前列腺癌细胞</subject><subject>细胞凋亡</subject><subject>薯蓣皂甙</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2O1DAQhS0EYoaBA7BBZo0M_kmcZNkEhkFqYBawtqptp9tNYofYgckNODZuZWh2rFxSve-5qh5Czxl9zSit3kTGykYSygrCeCHI8gBdsqYRhImSP8y1rDihlRAX6EmMR0rLSvLiMbrgRdFI2bBL9PudC1E7T5w3s7YGb8YwphBdxKHDN_MAHm8_t3CLb6cQEySLW5gyEAbAre37iNNhCvP-gDc6uZ-QXPAntIU4QrREYPAGfwpm7s-9t7on_GSYrPP4GgbXL0_Row76aJ_dv1fo2_X7r-0N2X758LHdbIkuqEykbvJ-TNbaCKtZSbWxnbasrAtLZW1kKQ2vqN1VIKumtIZpoPlUhksNteGNuEKvVt9f4Dvwe3UM8-Tzjyodl-_m7m6nLM8XpYxyltVsVeu8fZxsp8bJDTAtilF1ykCtGahMqFMGasnMi5UZ591gzZn4e_Qs4Ksg5pbf2-nfDP9zfXk_ySH4_Y_MnY2LuqJc5Jz_AJBRniQ</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>陈静 李辉敏 张学农 熊朝梅 阮金兰</creator><general>Springer Berlin Heidelberg</general><general>Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Key Laboratory of Natural Medicinal Chemistry and Resources Evaluation of Hubei Province, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><general>School of Basic Medical Science, Jiujiang University, Jiujiang 332000, China%Department of Pharmacy, Yichang Central People's Hospital & the First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20140201</creationdate><title>Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family</title><author>陈静 李辉敏 张学农 熊朝梅 阮金兰</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-89993168cd3ec150cdefce1584e068d656d270eb7a6795ed1ca0007d26ca8d293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis - drug effects</topic><topic>Bax</topic><topic>Bcl-2</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Bcl-2蛋白</topic><topic>Blotting, Western</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase-3</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Diosgenin - analogs & derivatives</topic><topic>Diosgenin - chemistry</topic><topic>Diosgenin - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enzyme Activation - drug effects</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>LNCaP细胞</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Molecular Structure</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Prostatic Neoplasms - pathology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Time Factors</topic><topic>前列腺癌细胞</topic><topic>细胞凋亡</topic><topic>薯蓣皂甙</topic><toplevel>online_resources</toplevel><creatorcontrib>陈静 李辉敏 张学农 熊朝梅 阮金兰</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - 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Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>陈静 李辉敏 张学农 熊朝梅 阮金兰</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><stitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</stitle><addtitle>Journal of Zuazhong University of Science and Technology： Medical Edition</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>34</volume><issue>1</issue><spage>125</spage><epage>130</epage><pages>125-130</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Dioscin is a natural steroid saponin derived from several plants, showing potent anti-cancer effect against a variety of tumor cell lines. In the present study, we investigated the anti-cancer activity of dioscin against human LNCaP cells, and evaluated the possible mechanism involved in its antineoplastic action. It was found that dioscin（1, 2 and 4 μmol/L） could significantly inhibit the viability of LNCaP cells in a time- and concentration-dependent manner. Flow cytometry revealed that the apoptosis rate was increased after treatment of LNCaP cells with dioscin for 24 h, indicating that apoptosis was an important mechanism by which dioscin inhibited cancer. Western blotting was employed to detect the expression of caspase-3, Bcl-2 and Bax in LNCaP cells. The expression of cleaved caspase-3 was significantly increased, and meanwhile procaspase-3 was markedly decreased. The expression of anti-apoptotic protein Bcl-2 was down-regulated, whereas the pro-apoptotic protein Bax was up-regulated. Moreover, the Bcl-2/Bax ratio was drastically decreased. These results suggested that dioscin possessed potential anti-tumor activity in human LNCaP cells through the apoptosis pathway, which might be associated with caspase-3 and Bcl-2 protein family.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>24496691</pmid><doi>10.1007/s11596-014-1243-y</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2014-02, Vol.34 (1), p.125-130 |
| issn | 1672-0733 1993-1352 |
| language | eng |
| recordid | cdi_wanfang_journals_tjykdxxb_e201401021 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Apoptosis - drug effects Bax Bcl-2 bcl-2-Associated X Protein - metabolism Bcl-2蛋白 Blotting, Western Caspase 3 - metabolism Caspase-3 Cell Line, Tumor Cell Survival - drug effects Diosgenin - analogs & derivatives Diosgenin - chemistry Diosgenin - pharmacology Dose-Response Relationship, Drug Enzyme Activation - drug effects Flow Cytometry Humans LNCaP细胞 Male Medicine Medicine & Public Health Molecular Structure Prostatic Neoplasms - metabolism Prostatic Neoplasms - pathology Proto-Oncogene Proteins c-bcl-2 - metabolism Time Factors 前列腺癌细胞 细胞凋亡 薯蓣皂甙 |
| title | Dioscin-induced Apoptosis of Human LNCaP Prostate Carcinoma Cells through Activation of Caspase-3 and Modulation of Bcl-2 Protein Family |
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