The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke
In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2008-04, Vol.28 (2), p.128-131 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
| container_volume | 28 |
| creator | 胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平 |
| description | In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by immunohistochemically measured. The mRNA expression in the lung tissues was determined by reverse transcription polymerase chain reaction （RT-PCR）. The number of cells positive for SPA of the S group （0.52±0.05） was lower than that of the N group （0.72±0.06） （P〈0.05）. The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease. |
| doi_str_mv | 10.1007/s11596-008-0203-9 |
| format | Article |
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The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by immunohistochemically measured. The mRNA expression in the lung tissues was determined by reverse transcription polymerase chain reaction （RT-PCR）. The number of cells positive for SPA of the S group （0.52±0.05） was lower than that of the N group （0.72±0.06） （P〈0.05）. The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-008-0203-9</identifier><identifier>PMID: 18480979</identifier><language>eng</language><publisher>Heidelberg: Huazhong University of Science and Technology</publisher><subject>Animals ; Gene Expression Regulation ; Immunohistochemistry - methods ; Lung - metabolism ; Male ; Medicine ; Medicine & Public Health ; Microscopy, Electron ; Pulmonary Surfactant-Associated Protein A - biosynthesis ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Smoking - adverse effects ; Tobacco Smoke Pollution - adverse effects ; 呼吸系统 ; 蛋白质 ; 表面活性物质</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2008-04, Vol.28 (2), p.128-131</ispartof><rights>Huazhong University of Science and Technology and Springer-Verlag GmbH 2008</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-49dfe734a1966e75b354adc246287353e2c59f71d677406bef163207a3e517c23</citedby><cites>FETCH-LOGICAL-c436t-49dfe734a1966e75b354adc246287353e2c59f71d677406bef163207a3e517c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18480979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平</creatorcontrib><title>The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</addtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>In order to confirm the alteration and significance of cigarette smoke exposure on SP-A in rats, 20 Wistar rats were assigned randomly to two groups： an N group （n=10）, and an S group （n=10）. The ultra-structural change was observed by electron microscopy. The number of cells positive for SPA was by immunohistochemically measured. The mRNA expression in the lung tissues was determined by reverse transcription polymerase chain reaction （RT-PCR）. The number of cells positive for SPA of the S group （0.52±0.05） was lower than that of the N group （0.72±0.06） （P〈0.05）. The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease.</description><subject>Animals</subject><subject>Gene Expression Regulation</subject><subject>Immunohistochemistry - methods</subject><subject>Lung - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy, Electron</subject><subject>Pulmonary Surfactant-Associated Protein A - biosynthesis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Smoking - adverse effects</subject><subject>Tobacco Smoke Pollution - adverse effects</subject><subject>呼吸系统</subject><subject>蛋白质</subject><subject>表面活性物质</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtrGzEUhUVpadK0P6CbIrropkyqx4xkLY1JHxBIiNO10GiuxmOPJUfSEPvfV2EM2XUjCfSdcy_nIPSZkmtKiPyRKG2UqAhZVIQRXqk36JIqxSvKG_a2vIVkFZGcX6APKW0JaaRg9Xt0QRf1giipLpF73ABejhmiyUPw2PgOr4feD26wxlvAweH1FJ2x2fiM72PIMHi8xOV4MDnh1SYGX9hxPOGb4yEk6HAOeDX0JkLOgNf7sIOP6J0zY4JP5_sK_f1587j6Xd3e_fqzWt5WtuYiV7XqHEheG6qEANm0vKlNZ1kt2ELyhgOzjXKSdkLKmogWHBWcEWk4NFRaxq_Q99n32XhnfK-3YYq-TNR5e9p1x2OrgZW8SlyEF_rbTB9ieJogZb0fkoVxNB7ClDQjitaUywLSGbQxpBTB6UMc9iaeNCX6pQo9V6GLtX6pQqui-XI2n9o9dK-Kc_YFYDOQypfvIb4u-z_Xr-dNNsH3T0WnW2N3bhhBM0mYKm3zf0W5nk0</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平</creator><general>Huazhong University of Science and Technology</general><general>Department of Respiratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Key Laboratory of Respiratory Disease of Ministry of Public Health, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20080401</creationdate><title>The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke</title><author>胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-49dfe734a1966e75b354adc246287353e2c59f71d677406bef163207a3e517c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Gene Expression Regulation</topic><topic>Immunohistochemistry - methods</topic><topic>Lung - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy, Electron</topic><topic>Pulmonary Surfactant-Associated Protein A - biosynthesis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Smoking - adverse effects</topic><topic>Tobacco Smoke Pollution - adverse effects</topic><topic>呼吸系统</topic><topic>蛋白质</topic><topic>表面活性物质</topic><toplevel>online_resources</toplevel><creatorcontrib>胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>胡琼洁 张惠兰 熊盛道 史雪梅 徐永健 张珍祥 甄国华 赵建平</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><stitle>J. Huazhong Univ. Sci. Technol. [Med. 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The levels of mRNA of SPA in the lung tissues of the S group （0.3522±0.0512） was significantly lower than that of the N group （0.4432± 0.05628） （P〈0.05）. It is concluded that cigarette smoke alone decreased the level of SP-A and that might have an important effect on surfactant metabolism and the host defense functions of surfactant in the peripheral airways, which might play a crucial role in the development of chronic obstructive lung disease.</abstract><cop>Heidelberg</cop><pub>Huazhong University of Science and Technology</pub><pmid>18480979</pmid><doi>10.1007/s11596-008-0203-9</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2008-04, Vol.28 (2), p.128-131 |
| issn | 1672-0733 1993-1352 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Gene Expression Regulation Immunohistochemistry - methods Lung - metabolism Male Medicine Medicine & Public Health Microscopy, Electron Pulmonary Surfactant-Associated Protein A - biosynthesis Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Smoking - adverse effects Tobacco Smoke Pollution - adverse effects 呼吸系统 蛋白质 表面活性物质 |
| title | The Alteration and Significance of Surfactant Protein A in Rats Chronically Exposed to Cigarette Smoke |
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