Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve
This study is to investigate the effect of local phVEGF(165) injection on sciatic nerve regeneration in the rats and to search for a new way in the further treatment of peripheral nerve injuries. Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequen...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2007-04, Vol.27 (2), p.186-189 |
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| creator | Fu, Chongyang Hong, Guangxiang Wang, Fabin |
| description | This study is to investigate the effect of local phVEGF(165) injection on sciatic nerve regeneration in the rats and to search for a new way in the further treatment of peripheral nerve injuries. Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequently reconnected with 10/0 epineurial nylon sutures. The injured segments was locally injected with normal saline (group A), or 25 microg of phVEGF(165) (group B) or 50 microg phVEGF(165) (group C). Nerve conduction and regeneration were evaluated in terms of the histological changes, weight of gastrocnemius muscles, electrophysiology and morphometric results. Our study demonstrated that rats of group C showed the best results in terms of nerve regeneration, followed by group B and group A. Our findings suggested that local injection of phVEGF165 can facilitate nerve regeneration and promote functional recovery in a dose-dependent manner. |
| doi_str_mv | 10.1007/s11596-007-0221-z |
| format | Article |
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Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequently reconnected with 10/0 epineurial nylon sutures. The injured segments was locally injected with normal saline (group A), or 25 microg of phVEGF(165) (group B) or 50 microg phVEGF(165) (group C). Nerve conduction and regeneration were evaluated in terms of the histological changes, weight of gastrocnemius muscles, electrophysiology and morphometric results. Our study demonstrated that rats of group C showed the best results in terms of nerve regeneration, followed by group B and group A. Our findings suggested that local injection of phVEGF165 can facilitate nerve regeneration and promote functional recovery in a dose-dependent manner.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-007-0221-z</identifier><identifier>PMID: 17497293</identifier><language>eng</language><publisher>China: Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China%Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China</publisher><subject>Action Potentials ; Animals ; Axons - metabolism ; Axons - physiology ; Electrophysiology ; Genetic Therapy - methods ; Male ; Muscle, Skeletal - innervation ; Muscle, Skeletal - pathology ; Muscle, Skeletal - physiopathology ; Nerve Regeneration - genetics ; Nerve Regeneration - physiology ; Plasmids - administration & dosage ; Plasmids - genetics ; Rats ; Rats, Wistar ; Sciatic Nerve - injuries ; Sciatic Nerve - physiopathology ; Vascular Endothelial Growth Factor A - genetics ; Vascular Endothelial Growth Factor A - physiology</subject><ispartof>Journal of Huazhong University of Science and Technology. 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All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-4692bd5a4b3f3dc8a020cb39f8e08aed1026cb7e7000a032837565480a8a4eb33</citedby><cites>FETCH-LOGICAL-c366t-4692bd5a4b3f3dc8a020cb39f8e08aed1026cb7e7000a032837565480a8a4eb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/tjykdxxb-e/tjykdxxb-e.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17497293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Chongyang</creatorcontrib><creatorcontrib>Hong, Guangxiang</creatorcontrib><creatorcontrib>Wang, Fabin</creatorcontrib><title>Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><description>This study is to investigate the effect of local phVEGF(165) injection on sciatic nerve regeneration in the rats and to search for a new way in the further treatment of peripheral nerve injuries. Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequently reconnected with 10/0 epineurial nylon sutures. The injured segments was locally injected with normal saline (group A), or 25 microg of phVEGF(165) (group B) or 50 microg phVEGF(165) (group C). Nerve conduction and regeneration were evaluated in terms of the histological changes, weight of gastrocnemius muscles, electrophysiology and morphometric results. Our study demonstrated that rats of group C showed the best results in terms of nerve regeneration, followed by group B and group A. Our findings suggested that local injection of phVEGF165 can facilitate nerve regeneration and promote functional recovery in a dose-dependent manner.</description><subject>Action Potentials</subject><subject>Animals</subject><subject>Axons - metabolism</subject><subject>Axons - physiology</subject><subject>Electrophysiology</subject><subject>Genetic Therapy - methods</subject><subject>Male</subject><subject>Muscle, Skeletal - innervation</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Nerve Regeneration - genetics</subject><subject>Nerve Regeneration - physiology</subject><subject>Plasmids - administration & dosage</subject><subject>Plasmids - genetics</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sciatic Nerve - injuries</subject><subject>Sciatic Nerve - physiopathology</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Vascular Endothelial Growth Factor A - physiology</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1LxDAQDaL4sfoDvEhOXqQ6SdqkOYrsqiB4UW8S0nSqXbuNJl1d99ebdRc8CgPzMu_NY8gj5JjBOQNQF5GxQssswQw4Z9lyi-wzrUXGRMG3E5aKZ6CE2CMHMU4BCiV5vkv2mMq14lrsk-eJ_fTBVh1SbBp0A_UN7byzHX0aX0_oC_ZI236amNb3NJVd-D6xAVdUsL_jtqfDK9L0otG1aeZo4j7xkOw0tot4tOkj8jgZP1zdZHf317dXl3eZE1IOWS41r-rC5pVoRO1KCxxcJXRTIpQWawZcukqhAgALgpdCFbLIS7ClzbESYkTO1r5ftm9s_2Kmfh7SldEM0--3erGoDPL0TcmXs6Q-Xavfg_-YYxzMrI0Ou8726OfRKMhLCfC_kOlS5-mUJGRroQs-xoCNeQ_tzIZvw8CsojLrqMwKrqIyy7RzsjGfVzOs_zY22Ygf7EOOcQ</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Fu, Chongyang</creator><creator>Hong, Guangxiang</creator><creator>Wang, Fabin</creator><general>Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China%Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20070401</creationdate><title>Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve</title><author>Fu, Chongyang ; Hong, Guangxiang ; Wang, Fabin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-4692bd5a4b3f3dc8a020cb39f8e08aed1026cb7e7000a032837565480a8a4eb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Action Potentials</topic><topic>Animals</topic><topic>Axons - metabolism</topic><topic>Axons - physiology</topic><topic>Electrophysiology</topic><topic>Genetic Therapy - methods</topic><topic>Male</topic><topic>Muscle, Skeletal - innervation</topic><topic>Muscle, Skeletal - pathology</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Nerve Regeneration - genetics</topic><topic>Nerve Regeneration - physiology</topic><topic>Plasmids - administration & dosage</topic><topic>Plasmids - genetics</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Nerve - physiopathology</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Vascular Endothelial Growth Factor A - physiology</topic><toplevel>online_resources</toplevel><creatorcontrib>Fu, Chongyang</creatorcontrib><creatorcontrib>Hong, Guangxiang</creatorcontrib><creatorcontrib>Wang, Fabin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Chongyang</au><au>Hong, Guangxiang</au><au>Wang, Fabin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>27</volume><issue>2</issue><spage>186</spage><epage>189</epage><pages>186-189</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>This study is to investigate the effect of local phVEGF(165) injection on sciatic nerve regeneration in the rats and to search for a new way in the further treatment of peripheral nerve injuries. Forty-five adult male Wistar rats received a neurotomy to bilateral sciatic nerves, which were subsequently reconnected with 10/0 epineurial nylon sutures. The injured segments was locally injected with normal saline (group A), or 25 microg of phVEGF(165) (group B) or 50 microg phVEGF(165) (group C). Nerve conduction and regeneration were evaluated in terms of the histological changes, weight of gastrocnemius muscles, electrophysiology and morphometric results. Our study demonstrated that rats of group C showed the best results in terms of nerve regeneration, followed by group B and group A. Our findings suggested that local injection of phVEGF165 can facilitate nerve regeneration and promote functional recovery in a dose-dependent manner.</abstract><cop>China</cop><pub>Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China%Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China</pub><pmid>17497293</pmid><doi>10.1007/s11596-007-0221-z</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2007-04, Vol.27 (2), p.186-189 |
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| subjects | Action Potentials Animals Axons - metabolism Axons - physiology Electrophysiology Genetic Therapy - methods Male Muscle, Skeletal - innervation Muscle, Skeletal - pathology Muscle, Skeletal - physiopathology Nerve Regeneration - genetics Nerve Regeneration - physiology Plasmids - administration & dosage Plasmids - genetics Rats Rats, Wistar Sciatic Nerve - injuries Sciatic Nerve - physiopathology Vascular Endothelial Growth Factor A - genetics Vascular Endothelial Growth Factor A - physiology |
| title | Favorable effect of local VEGF gene injection on axonal regeneration in the rat sciatic nerve |
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