Comparison of cell deaths induced by transmembrane and secretory TNF-alpha
Our previous study showed that transmembrane TNF-alpha (TM-TNF-alpha) had broader tumoricidal spectrum than secretory TNF-alpha (s-TNF-alpha). This study examined the difference between the two kinds of TNF-alpha in inducing cells and the relationship between the apoptosis induced by TM-TNF-alpha an...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2007-04, Vol.27 (2), p.117-119 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | Yang, Lin Shi, Wenfang Wang, Jing Jiang, Xiaodan Feng, Wei Li, Zuoya |
| description | Our previous study showed that transmembrane TNF-alpha (TM-TNF-alpha) had broader tumoricidal spectrum than secretory TNF-alpha (s-TNF-alpha). This study examined the difference between the two kinds of TNF-alpha in inducing cells and the relationship between the apoptosis induced by TM-TNF-alpha and the cell cycle. Bioassay was employed to compare the cytotoxic effect of two kinds of TNF-alpha on cell lines L-929 and HepG2. TUNEL was used to detect apoptosis and the TdT and PI co-staining were used for determining the phase of apoptotic cells. Our results showed that TM-TNF-alpha could kill not only s-TNF-sensitive L929 cells but also s-TNF-tolerant HepG2 cells. TM-TNF-alpha predominantly induced apoptosis while s-TNF could induce both apoptosis and necrosis. The apoptosis of L-929 cells induced by TM-TNF-alpha mainly occurred in S phase and the apoptosis of HepG2 predominantly took place in G(1) phase. It is concluded that the cytotoxic effects of the two TNF differ substantially. Since TM-TNF-alpha works locally, mainly induces apoptosis and has broader anti-tumor spectrum, it may be more effective for the treatment of tumor than s-TNF. |
| doi_str_mv | 10.1007/s11596-007-0201-3 |
| format | Article |
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This study examined the difference between the two kinds of TNF-alpha in inducing cells and the relationship between the apoptosis induced by TM-TNF-alpha and the cell cycle. Bioassay was employed to compare the cytotoxic effect of two kinds of TNF-alpha on cell lines L-929 and HepG2. TUNEL was used to detect apoptosis and the TdT and PI co-staining were used for determining the phase of apoptotic cells. Our results showed that TM-TNF-alpha could kill not only s-TNF-sensitive L929 cells but also s-TNF-tolerant HepG2 cells. TM-TNF-alpha predominantly induced apoptosis while s-TNF could induce both apoptosis and necrosis. The apoptosis of L-929 cells induced by TM-TNF-alpha mainly occurred in S phase and the apoptosis of HepG2 predominantly took place in G(1) phase. It is concluded that the cytotoxic effects of the two TNF differ substantially. Since TM-TNF-alpha works locally, mainly induces apoptosis and has broader anti-tumor spectrum, it may be more effective for the treatment of tumor than s-TNF.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-007-0201-3</identifier><identifier>PMID: 17497273</identifier><language>eng</language><publisher>China: Department of lmmunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China</publisher><subject>Animals ; Apoptosis - drug effects ; Carcinoma, Hepatocellular - metabolism ; Carcinoma, Hepatocellular - pathology ; Cell Line ; Cell Line, Tumor ; G1 Phase - drug effects ; Humans ; Liver Neoplasms - metabolism ; Liver Neoplasms - pathology ; Membrane Proteins - pharmacology ; Membrane Proteins - secretion ; Mice ; S Phase - drug effects ; Tumor Necrosis Factor-alpha - pharmacology ; Tumor Necrosis Factor-alpha - secretion</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2007-04, Vol.27 (2), p.117-119</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-bc0ca3cced67eb802154478fbea1c91fdde6962a1666876cd29d9f33ce5db76f3</citedby><cites>FETCH-LOGICAL-c366t-bc0ca3cced67eb802154478fbea1c91fdde6962a1666876cd29d9f33ce5db76f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/tjykdxxb-e/tjykdxxb-e.jpg</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17497273$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Lin</creatorcontrib><creatorcontrib>Shi, Wenfang</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Jiang, Xiaodan</creatorcontrib><creatorcontrib>Feng, Wei</creatorcontrib><creatorcontrib>Li, Zuoya</creatorcontrib><title>Comparison of cell deaths induced by transmembrane and secretory TNF-alpha</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><description>Our previous study showed that transmembrane TNF-alpha (TM-TNF-alpha) had broader tumoricidal spectrum than secretory TNF-alpha (s-TNF-alpha). This study examined the difference between the two kinds of TNF-alpha in inducing cells and the relationship between the apoptosis induced by TM-TNF-alpha and the cell cycle. Bioassay was employed to compare the cytotoxic effect of two kinds of TNF-alpha on cell lines L-929 and HepG2. TUNEL was used to detect apoptosis and the TdT and PI co-staining were used for determining the phase of apoptotic cells. Our results showed that TM-TNF-alpha could kill not only s-TNF-sensitive L929 cells but also s-TNF-tolerant HepG2 cells. TM-TNF-alpha predominantly induced apoptosis while s-TNF could induce both apoptosis and necrosis. The apoptosis of L-929 cells induced by TM-TNF-alpha mainly occurred in S phase and the apoptosis of HepG2 predominantly took place in G(1) phase. It is concluded that the cytotoxic effects of the two TNF differ substantially. Since TM-TNF-alpha works locally, mainly induces apoptosis and has broader anti-tumor spectrum, it may be more effective for the treatment of tumor than s-TNF.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma, Hepatocellular - metabolism</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>G1 Phase - drug effects</subject><subject>Humans</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - pathology</subject><subject>Membrane Proteins - pharmacology</subject><subject>Membrane Proteins - secretion</subject><subject>Mice</subject><subject>S Phase - drug effects</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - secretion</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT9PwzAQxS0EolD4ACzIEwsy-OzEbkZUUf6ogqXMlmM7tKWJg52I9tvjqJUYme4Nv3unew-hK6B3QKm8jwB5IUiShDIKhB-hMygKToDn7DhpIRmhkvMROo9xTWkuBctO0QhkVkgm-Rl6nfq61WEVfYN9hY3bbLB1ultGvGpsb5zF5Q53QTexdnWZpsO6sTg6E1znww4v3mZEb9qlvkAnld5Ed3mYY_Qxe1xMn8n8_ell-jAnhgvRkdJQo7lJzkK6ckIZ5FkmJ1XpNJgCKmudKATTIISYSGEsK2xRcW5cbkspKj5Gt3vfH91UuvlUa9-HJl1U3Xr3ZbfbUjmWMkmRUEj0zZ5ug__uXexUvYrDm-kT30claU4BqPgXZDSTgvMsgbAHTfAxBlepNqxqHXYKqBp6Ufte1CCHXhRPO9cH876snf3bOBTBfwFXcYiM</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Yang, Lin</creator><creator>Shi, Wenfang</creator><creator>Wang, Jing</creator><creator>Jiang, Xiaodan</creator><creator>Feng, Wei</creator><creator>Li, Zuoya</creator><general>Department of lmmunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20070401</creationdate><title>Comparison of cell deaths induced by transmembrane and secretory TNF-alpha</title><author>Yang, Lin ; Shi, Wenfang ; Wang, Jing ; Jiang, Xiaodan ; Feng, Wei ; Li, Zuoya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-bc0ca3cced67eb802154478fbea1c91fdde6962a1666876cd29d9f33ce5db76f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma, Hepatocellular - metabolism</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>G1 Phase - drug effects</topic><topic>Humans</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - pathology</topic><topic>Membrane Proteins - pharmacology</topic><topic>Membrane Proteins - secretion</topic><topic>Mice</topic><topic>S Phase - drug effects</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - secretion</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang, Lin</creatorcontrib><creatorcontrib>Shi, Wenfang</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Jiang, Xiaodan</creatorcontrib><creatorcontrib>Feng, Wei</creatorcontrib><creatorcontrib>Li, Zuoya</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Lin</au><au>Shi, Wenfang</au><au>Wang, Jing</au><au>Jiang, Xiaodan</au><au>Feng, Wei</au><au>Li, Zuoya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of cell deaths induced by transmembrane and secretory TNF-alpha</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>J Huazhong Univ Sci Technolog Med Sci</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>27</volume><issue>2</issue><spage>117</spage><epage>119</epage><pages>117-119</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Our previous study showed that transmembrane TNF-alpha (TM-TNF-alpha) had broader tumoricidal spectrum than secretory TNF-alpha (s-TNF-alpha). This study examined the difference between the two kinds of TNF-alpha in inducing cells and the relationship between the apoptosis induced by TM-TNF-alpha and the cell cycle. Bioassay was employed to compare the cytotoxic effect of two kinds of TNF-alpha on cell lines L-929 and HepG2. TUNEL was used to detect apoptosis and the TdT and PI co-staining were used for determining the phase of apoptotic cells. Our results showed that TM-TNF-alpha could kill not only s-TNF-sensitive L929 cells but also s-TNF-tolerant HepG2 cells. TM-TNF-alpha predominantly induced apoptosis while s-TNF could induce both apoptosis and necrosis. The apoptosis of L-929 cells induced by TM-TNF-alpha mainly occurred in S phase and the apoptosis of HepG2 predominantly took place in G(1) phase. It is concluded that the cytotoxic effects of the two TNF differ substantially. Since TM-TNF-alpha works locally, mainly induces apoptosis and has broader anti-tumor spectrum, it may be more effective for the treatment of tumor than s-TNF.</abstract><cop>China</cop><pub>Department of lmmunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China</pub><pmid>17497273</pmid><doi>10.1007/s11596-007-0201-3</doi><tpages>3</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Carcinoma, Hepatocellular - metabolism Carcinoma, Hepatocellular - pathology Cell Line Cell Line, Tumor G1 Phase - drug effects Humans Liver Neoplasms - metabolism Liver Neoplasms - pathology Membrane Proteins - pharmacology Membrane Proteins - secretion Mice S Phase - drug effects Tumor Necrosis Factor-alpha - pharmacology Tumor Necrosis Factor-alpha - secretion |
| title | Comparison of cell deaths induced by transmembrane and secretory TNF-alpha |
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