Self-assembled IKVAV Peptide Nanofibers Promote Adherence of PC12 Cells
Lack of biocompatibility and bioactivity is a big problem for the synthetic materials that have been generated for neural tissue engineering. To get around the problem and generate better scaffold for neural tissue repair, we intended to generate nano-fibers by self-assembly of polypeptide IKVAV. Bi...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2006, Vol.26 (5), p.594-596 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 吴永超 郑启新 杜靖远 宋玉林 吴斌 郭晓东 |
| description | Lack of biocompatibility and bioactivity is a big problem for the synthetic materials that have been generated for neural tissue engineering. To get around the problem and generate better scaffold for neural tissue repair, we intended to generate nano-fibers by self-assembly of polypeptide IKVAV. Bioactive IKVAV Peptide-Amphiphile （IKVAV-PA） was first synthesized and purified, the property of which was analyzed and determined by high-performance liquid chromatography （HPLC） and mass spectrometry （MS）. Then, by addition of hydrogen chloride （HC1）, self-assembly of IKVAV-PA was induced in vitro and nano-fibers formed as shown by transmission electron microscopy （TEM）. The effect of IKVAV nanofibers on adherence of PCI2 cells was assayed in cell culture and the results showed that the rates of adherence of PC12 increased significantly when the density of IKVAV was within a certain range （0.58 μg/cm^2 to 15.6 μg/cm^2）. However, its effect on the rates of adherence did not significantly alter with time, whether after 1 hour or 3 hours of culture. In general, we showed that IKVAV-PA can successfully self-assemble to form nanofiber, and promote rapid and stable adherence of PC12 cells, and the effect of the self-assembled IKVAV to promote PCI2 cells adherence is dosage-dependent within a certain range of densities. |
| doi_str_mv | 10.1007/s11596-006-0530-7 |
| format | Article |
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To get around the problem and generate better scaffold for neural tissue repair, we intended to generate nano-fibers by self-assembly of polypeptide IKVAV. Bioactive IKVAV Peptide-Amphiphile （IKVAV-PA） was first synthesized and purified, the property of which was analyzed and determined by high-performance liquid chromatography （HPLC） and mass spectrometry （MS）. Then, by addition of hydrogen chloride （HC1）, self-assembly of IKVAV-PA was induced in vitro and nano-fibers formed as shown by transmission electron microscopy （TEM）. The effect of IKVAV nanofibers on adherence of PCI2 cells was assayed in cell culture and the results showed that the rates of adherence of PC12 increased significantly when the density of IKVAV was within a certain range （0.58 μg/cm^2 to 15.6 μg/cm^2）. However, its effect on the rates of adherence did not significantly alter with time, whether after 1 hour or 3 hours of culture. In general, we showed that IKVAV-PA can successfully self-assemble to form nanofiber, and promote rapid and stable adherence of PC12 cells, and the effect of the self-assembled IKVAV to promote PCI2 cells adherence is dosage-dependent within a certain range of densities.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-006-0530-7</identifier><identifier>PMID: 17219978</identifier><language>eng</language><publisher>China: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China</publisher><subject>Animals ; Biocompatible Materials - chemistry ; Cell Adhesion - drug effects ; Laminin - chemistry ; Nanofibers - chemistry ; Nerve Regeneration ; Neurons - cytology ; PC12 Cells ; Peptide Fragments - chemistry ; Rats ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; 神经系统</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (5), p.594-596</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2780-362d09ca7abb54ff1d58ba86cd67b133f2b2636aa425fd7d397c132cb54284a43</citedby><cites>FETCH-LOGICAL-c2780-362d09ca7abb54ff1d58ba86cd67b133f2b2636aa425fd7d397c132cb54284a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17219978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>吴永超 郑启新 杜靖远 宋玉林 吴斌 郭晓东</creatorcontrib><title>Self-assembled IKVAV Peptide Nanofibers Promote Adherence of PC12 Cells</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>Lack of biocompatibility and bioactivity is a big problem for the synthetic materials that have been generated for neural tissue engineering. To get around the problem and generate better scaffold for neural tissue repair, we intended to generate nano-fibers by self-assembly of polypeptide IKVAV. Bioactive IKVAV Peptide-Amphiphile （IKVAV-PA） was first synthesized and purified, the property of which was analyzed and determined by high-performance liquid chromatography （HPLC） and mass spectrometry （MS）. Then, by addition of hydrogen chloride （HC1）, self-assembly of IKVAV-PA was induced in vitro and nano-fibers formed as shown by transmission electron microscopy （TEM）. The effect of IKVAV nanofibers on adherence of PCI2 cells was assayed in cell culture and the results showed that the rates of adherence of PC12 increased significantly when the density of IKVAV was within a certain range （0.58 μg/cm^2 to 15.6 μg/cm^2）. However, its effect on the rates of adherence did not significantly alter with time, whether after 1 hour or 3 hours of culture. In general, we showed that IKVAV-PA can successfully self-assemble to form nanofiber, and promote rapid and stable adherence of PC12 cells, and the effect of the self-assembled IKVAV to promote PCI2 cells adherence is dosage-dependent within a certain range of densities.</description><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Adhesion - drug effects</subject><subject>Laminin - chemistry</subject><subject>Nanofibers - chemistry</subject><subject>Nerve Regeneration</subject><subject>Neurons - cytology</subject><subject>PC12 Cells</subject><subject>Peptide Fragments - chemistry</subject><subject>Rats</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds - chemistry</subject><subject>神经系统</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1v1DAQhi0EomXhB3BBERcOVcCeSezkuFrRUlG1KwG9Wv4Yt9km8dbeFe2_x9WuxGE0c3jmndHD2EfBvwrO1bcsRNvLmvNSLfJavWKnou-xFtjC6zJLBTVXiCfsXc4bzlsloXnLToSCwqnulF38ojHUJmea7Ei-uvx5u7yt1rTdDZ6qazPHMFhKuVqnOMUdVUt_T4lmR1UM1XoloFrROOb37E0wY6YPx75gf86__179qK9uLi5Xy6vagep4jRI8751Rxtq2CUH4trOmk85LZQViAAsSpTENtMErj71yAsEVGLrGNLhgZ4fcv2YOZr7Tm7hPc7mod5vnB__0ZDVBEcJbjrzQXw70NsXHPeWdnobsyr9mprjPWmEjmq5DWUhxIF2KOScKepuGyaRnLbh-ka0PsnXJ1i-yy-6CfTqm7-1E_v_G0W4BPh9D7-N89ziUd61xD2EYSQMCNAAC_wFl5YMW</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>吴永超 郑启新 杜靖远 宋玉林 吴斌 郭晓东</creator><general>Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2006</creationdate><title>Self-assembled IKVAV Peptide Nanofibers Promote Adherence of PC12 Cells</title><author>吴永超 郑启新 杜靖远 宋玉林 吴斌 郭晓东</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2780-362d09ca7abb54ff1d58ba86cd67b133f2b2636aa425fd7d397c132cb54284a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Adhesion - drug effects</topic><topic>Laminin - chemistry</topic><topic>Nanofibers - chemistry</topic><topic>Nerve Regeneration</topic><topic>Neurons - cytology</topic><topic>PC12 Cells</topic><topic>Peptide Fragments - chemistry</topic><topic>Rats</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds - chemistry</topic><topic>神经系统</topic><toplevel>online_resources</toplevel><creatorcontrib>吴永超 郑启新 杜靖远 宋玉林 吴斌 郭晓东</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. 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| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (5), p.594-596 |
| issn | 1672-0733 1993-1352 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biocompatible Materials - chemistry Cell Adhesion - drug effects Laminin - chemistry Nanofibers - chemistry Nerve Regeneration Neurons - cytology PC12 Cells Peptide Fragments - chemistry Rats Tissue Engineering - methods Tissue Scaffolds - chemistry 神经系统 |
| title | Self-assembled IKVAV Peptide Nanofibers Promote Adherence of PC12 Cells |
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