Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism
The influence of L-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatme...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2006, Vol.26 (5), p.524-527 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 张建初 夏蕾 张晓菊 杨卫兵 白明 |
| description | The influence of L-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and L-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, L-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism. |
| doi_str_mv | 10.1007/s11596-006-0509-4 |
| format | Article |
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Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and L-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, L-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-006-0509-4</identifier><identifier>PMID: 17219957</identifier><language>eng</language><publisher>China: Department of Pulmonary Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China%Department of Pulmonary Medicine, Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, China</publisher><subject>Animals ; Arginine - pharmacology ; Arginine - therapeutic use ; Cyclooxygenase 2 - genetics ; Cyclooxygenase 2 - metabolism ; Female ; L-精氨酸 ; Lung - enzymology ; Male ; Nitric Oxide Synthase Type III - genetics ; Nitric Oxide Synthase Type III - metabolism ; Pulmonary Embolism - drug therapy ; Pulmonary Embolism - enzymology ; Rats ; Rats, Wistar ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; 基因表达 ; 肺血管栓塞</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (5), p.524-527</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2789-12dfc9a3d88a4bd9b271d69f2e8d9521f6a0f68a77cabb3a4a341cd6f05dc2d3</citedby><cites>FETCH-LOGICAL-c2789-12dfc9a3d88a4bd9b271d69f2e8d9521f6a0f68a77cabb3a4a341cd6f05dc2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,778,782,4012,27906,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17219957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>张建初 夏蕾 张晓菊 杨卫兵 白明</creatorcontrib><title>Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>The influence of L-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and L-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, L-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism.</description><subject>Animals</subject><subject>Arginine - pharmacology</subject><subject>Arginine - therapeutic use</subject><subject>Cyclooxygenase 2 - genetics</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Female</subject><subject>L-精氨酸</subject><subject>Lung - enzymology</subject><subject>Male</subject><subject>Nitric Oxide Synthase Type III - genetics</subject><subject>Nitric Oxide Synthase Type III - metabolism</subject><subject>Pulmonary Embolism - drug therapy</subject><subject>Pulmonary Embolism - enzymology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>基因表达</subject><subject>肺血管栓塞</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUU1rGzEQFaWl-Wh_QC9B9NJD2FYfu6vVMRg3CZi4UB96E1p92HJ2JUfyEuffd4wNOQzSMG_ezLyH0DdKflJCxK9CaSPbihCIhsiq_oAuqZS8orxhH-HfClYRwfkFuiplS0gjWlZ_RhdUMMA14hLZx-iHyUXjcPJ4Uem8DjFEyCLebxyeH3bZlRIghbp7Wv7FOlo8W_5jOMRj2eUwurjXA_4zDWOKOr_h1SansU8OYghl_II-eT0U9_X8XqPV7_lq9lAtlvePs7tFZZjoZEWZ9UZqbrtO172VPRPUttIz11nZMOpbTXzbaSGM7nuua81ramzrSWMNs_wa3Z5oX3X0Oq7VNk05wkC1374928OhV46BWKAVkYD-cULvcnqZXNmrMRTjhkFHl6aiBJDXXSdrQNIT0uRUSnZe7eBmuFNRoo5GqJMRCrjV0Qh17Lk5s0_96Ox7x1l5AHw_k25SXL8EWLfX5tmHwSnGGathU_4f9vePVA</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>张建初 夏蕾 张晓菊 杨卫兵 白明</creator><general>Department of Pulmonary Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China%Department of Pulmonary Medicine, Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2006</creationdate><title>Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism</title><author>张建初 夏蕾 张晓菊 杨卫兵 白明</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2789-12dfc9a3d88a4bd9b271d69f2e8d9521f6a0f68a77cabb3a4a341cd6f05dc2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Arginine - pharmacology</topic><topic>Arginine - therapeutic use</topic><topic>Cyclooxygenase 2 - genetics</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Female</topic><topic>L-精氨酸</topic><topic>Lung - enzymology</topic><topic>Male</topic><topic>Nitric Oxide Synthase Type III - genetics</topic><topic>Nitric Oxide Synthase Type III - metabolism</topic><topic>Pulmonary Embolism - drug therapy</topic><topic>Pulmonary Embolism - enzymology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>基因表达</topic><topic>肺血管栓塞</topic><toplevel>online_resources</toplevel><creatorcontrib>张建初 夏蕾 张晓菊 杨卫兵 白明</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>张建初 夏蕾 张晓菊 杨卫兵 白明</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2006</date><risdate>2006</risdate><volume>26</volume><issue>5</issue><spage>524</spage><epage>527</epage><pages>524-527</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>The influence of L-arginine on endothelial nitric oxide synthase （eNOS） and cyclooxygenase 2 （COX2） was observed in experimental pulmonary thromboembolism and the action mechanism on pulmonary thromboembolism was explored. Wistar rats were randomly divided into control group, model group and treatment group. Pulmonary thromboembolism models were established by auto-blood back transfusion, and L-Arg 100 mg/kg was intraperitoneally injected after successful model preparation. The animals were sacrificed at 3 h, 1 day, 3 days and 7 days after embolism. Plasma NO, TXB2 and 6-Keto-PGF1 α were detected. The expression of eNOS and COX2 protein and mRNA in pulmonary tissues was detected by immunohistochemistry and RT-PCR respectively. The results showed that pulmonary thrombosis could be seen post pulmonary embolism and inflammatory reaction was significant. Plasma NO was decreased （P〈0.01）, and the levels of TXB2, 6-Keto-PGF1α and T/P ratio were all elevated. The expression of eNOS protein and mRNA in the pulmonary tissue was down-regulated （P〈0.05）, while that of COX2 protein and mRNA was upregulated （P〈0.01）. In treatment group, the level of NO was increased, the levels of TXB2 and T/P ratio were decreased, but the level of 6-Keto-PGF1 α was increased. The expression of eNOS protein and mRNA in pulmonary tissue was upregulated （P〈0.05）, while that of COX2 protein and mRNA was down-regulated （P〈0.05）. In conclusion, L-arginine can educe the role of pulmonary tissue protection through up-regulating the expression of intra-pulmonary NOS and down -regulating COX2 in pulmonary thromboembolism.</abstract><cop>China</cop><pub>Department of Pulmonary Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China%Department of Pulmonary Medicine, Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, China</pub><pmid>17219957</pmid><doi>10.1007/s11596-006-0509-4</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (5), p.524-527 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Arginine - pharmacology Arginine - therapeutic use Cyclooxygenase 2 - genetics Cyclooxygenase 2 - metabolism Female L-精氨酸 Lung - enzymology Male Nitric Oxide Synthase Type III - genetics Nitric Oxide Synthase Type III - metabolism Pulmonary Embolism - drug therapy Pulmonary Embolism - enzymology Rats Rats, Wistar RNA, Messenger - genetics RNA, Messenger - metabolism 基因表达 肺血管栓塞 |
| title | Influence of L-arginine on the Expression of eNOS and COX2 in Experimental Pulmonary Thromboembolism |
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