P53 Regulation of Leukemia Cells with the Blockage of MDM2 by Antisense Oligonucleotides
Summary： The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group （MDM2 specific antisense oligonucleotides group）, cisplatin group, and combined treatment group. FCM analysis a...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2006, Vol.26 (4), p.414-416 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 房明浩 纪学梅 汤屹 刘文励 |
| description | Summary： The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group （MDM2 specific antisense oligonucleotides group）, cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression （P〈0.01） and increased the expression of P53 （P〈0.05）. Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin （P〈0.01）. It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy. |
| doi_str_mv | 10.1007/s11596-006-0409-7 |
| format | Article |
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Cells were divided into control group, AS group （MDM2 specific antisense oligonucleotides group）, cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression （P〈0.01） and increased the expression of P53 （P〈0.05）. Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin （P〈0.01）. It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/s11596-006-0409-7</identifier><identifier>PMID: 17120736</identifier><language>eng</language><publisher>China: Department of Internal Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China%Department of Chemotherapy and Radiation, Zhongnan Hospital, Wuhan University, Wuhan 430071, China%Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</publisher><subject>Apoptosis - drug effects ; Blotting, Western ; Cisplatin - pharmacology ; Flow Cytometry ; Gene Expression Regulation, Neoplastic - drug effects ; HL-60 Cells ; Humans ; Oligonucleotides, Antisense - genetics ; Proto-Oncogene Proteins c-mdm2 - biosynthesis ; Proto-Oncogene Proteins c-mdm2 - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; Tumor Suppressor Protein p53 - biosynthesis ; Tumor Suppressor Protein p53 - genetics ; Tumor Suppressor Protein p53 - physiology ; 低聚核苷酸 ; 基因治疗 ; 白血病 ; 肿瘤基因</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (4), p.414-416</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2609-8008453cfb8194a49888afa6271e164e4e3b3c5847134e195d8d04f1ee5ab7463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17120736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>房明浩 纪学梅 汤屹 刘文励</creatorcontrib><title>P53 Regulation of Leukemia Cells with the Blockage of MDM2 by Antisense Oligonucleotides</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>Summary： The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group （MDM2 specific antisense oligonucleotides group）, cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression （P〈0.01） and increased the expression of P53 （P〈0.05）. Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin （P〈0.01）. It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy.</description><subject>Apoptosis - drug effects</subject><subject>Blotting, Western</subject><subject>Cisplatin - pharmacology</subject><subject>Flow Cytometry</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>HL-60 Cells</subject><subject>Humans</subject><subject>Oligonucleotides, Antisense - genetics</subject><subject>Proto-Oncogene Proteins c-mdm2 - biosynthesis</subject><subject>Proto-Oncogene Proteins c-mdm2 - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transfection</subject><subject>Tumor Suppressor Protein p53 - biosynthesis</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Suppressor Protein p53 - physiology</subject><subject>低聚核苷酸</subject><subject>基因治疗</subject><subject>白血病</subject><subject>肿瘤基因</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxSMEoqXwAbggiwOXKtRjO_5zbJcClbZqhUDiZjnJJOvdrN3Gidr99ni1K3HswbJl_ebNm3lF8RHoV6BUXSSAysiS0nwENaV6VZyCMbwEXrHX-S0VK6ni_KR4l9Ka0kpJJt4WJ6CA5X95Wvy9rzj5hf08uMnHQGJHljhvcOsdWeAwJPLkpxWZVkiuhthsXI975vbbLSP1jlyGyScMCcnd4PsY5mbAOPkW0_viTeeGhB-O91nx5_v178XPcnn342ZxuSwbJrNlTakWFW-6WoMRThitteucZAoQpECBvOZNpYUCLhBM1eqWig4QK1crIflZcX7QfXKhc6G36ziPIXe003q3aZ-fa4ssbygviJpMfznQD2N8nDFNdutTk-d0AeOcrNSgKKiXwexEcy1YBuEANmNMacTOPox-68adBWr3KdlDSjZ7sPuUrMo1n47ic73F9n_FMZYMfD6KrmLoH30eq3bNpvMDWsaUURwY_wc6VZX2</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>房明浩 纪学梅 汤屹 刘文励</creator><general>Department of Internal Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China%Department of Chemotherapy and Radiation, Zhongnan Hospital, Wuhan University, Wuhan 430071, China%Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2006</creationdate><title>P53 Regulation of Leukemia Cells with the Blockage of MDM2 by Antisense Oligonucleotides</title><author>房明浩 纪学梅 汤屹 刘文励</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2609-8008453cfb8194a49888afa6271e164e4e3b3c5847134e195d8d04f1ee5ab7463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Apoptosis - drug effects</topic><topic>Blotting, Western</topic><topic>Cisplatin - pharmacology</topic><topic>Flow Cytometry</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>HL-60 Cells</topic><topic>Humans</topic><topic>Oligonucleotides, Antisense - genetics</topic><topic>Proto-Oncogene Proteins c-mdm2 - biosynthesis</topic><topic>Proto-Oncogene Proteins c-mdm2 - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transfection</topic><topic>Tumor Suppressor Protein p53 - biosynthesis</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumor Suppressor Protein p53 - physiology</topic><topic>低聚核苷酸</topic><topic>基因治疗</topic><topic>白血病</topic><topic>肿瘤基因</topic><toplevel>online_resources</toplevel><creatorcontrib>房明浩 纪学梅 汤屹 刘文励</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - 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FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression （P〈0.01） and increased the expression of P53 （P〈0.05）. Apoptosis rate were reduced by MDM2 specific antisense oligonucletides and cisplatin （P〈0.01）. 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| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (4), p.414-416 |
| issn | 1672-0733 1993-1352 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Apoptosis - drug effects Blotting, Western Cisplatin - pharmacology Flow Cytometry Gene Expression Regulation, Neoplastic - drug effects HL-60 Cells Humans Oligonucleotides, Antisense - genetics Proto-Oncogene Proteins c-mdm2 - biosynthesis Proto-Oncogene Proteins c-mdm2 - genetics Reverse Transcriptase Polymerase Chain Reaction Transfection Tumor Suppressor Protein p53 - biosynthesis Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - physiology 低聚核苷酸 基因治疗 白血病 肿瘤基因 |
| title | P53 Regulation of Leukemia Cells with the Blockage of MDM2 by Antisense Oligonucleotides |
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