Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro
Extracellular matrix （ECM） degradation is an essential step that allows tumor cells to penetrate a tissue barrier and become metastatic. Heparanase （HPSE） is an endoglycosidase that specifically degrades heparin sulfate proteoglycans （HSPG）, a chief component of ECM, HPSE is not expressed in normal...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2006, Vol.26 (1), p.72-74 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 高军 苏琳 秦仁义 常青 黄涛 冯延平 |
| description | Extracellular matrix （ECM） degradation is an essential step that allows tumor cells to penetrate a tissue barrier and become metastatic. Heparanase （HPSE） is an endoglycosidase that specifically degrades heparin sulfate proteoglycans （HSPG）, a chief component of ECM, HPSE is not expressed in normal epithelial cells but can be detected in a variety of human carcinomas including pancreatic cancer. In the present study, human pancreatic cancer cell line Panc-1 was transfected with HPSE antisense oligodeoxynucleotide （AS-ODN） in vitro, then the inhibitory effect of ASODN on HPSE gene expression and invasive ability of Panc-1 cells in vitro was examined. The HPSE mRNA and protein expression of Panc-1 cells transfected with AS-ODN was significantly inhibited. However, there were no marked inhibitory effects in Panc-1 cells treated with nonsense oligodeoxynucleotide （NS-ODN）. Moreover, a modified Boyden chamber assay demonstrated that transfection with HPSE AS-ODN significantly inhibited invasive potential of Panc-1 cells in vitro after AS-ODN transfection. This suggests that HPSE AS-ODN may contribute to the inhibition of HPSE mRNA and protein expression, and results in a decrease of the invasive ability of Panc-1 in vitro. |
| doi_str_mv | 10.1007/bf02828042 |
| format | Article |
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Heparanase （HPSE） is an endoglycosidase that specifically degrades heparin sulfate proteoglycans （HSPG）, a chief component of ECM, HPSE is not expressed in normal epithelial cells but can be detected in a variety of human carcinomas including pancreatic cancer. In the present study, human pancreatic cancer cell line Panc-1 was transfected with HPSE antisense oligodeoxynucleotide （AS-ODN） in vitro, then the inhibitory effect of ASODN on HPSE gene expression and invasive ability of Panc-1 cells in vitro was examined. The HPSE mRNA and protein expression of Panc-1 cells transfected with AS-ODN was significantly inhibited. However, there were no marked inhibitory effects in Panc-1 cells treated with nonsense oligodeoxynucleotide （NS-ODN）. Moreover, a modified Boyden chamber assay demonstrated that transfection with HPSE AS-ODN significantly inhibited invasive potential of Panc-1 cells in vitro after AS-ODN transfection. 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Medical sciences, 2006, Vol.26 (1), p.72-74</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16711012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>高军 苏琳 秦仁义 常青 黄涛 冯延平</creatorcontrib><title>Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>Extracellular matrix （ECM） degradation is an essential step that allows tumor cells to penetrate a tissue barrier and become metastatic. Heparanase （HPSE） is an endoglycosidase that specifically degrades heparin sulfate proteoglycans （HSPG）, a chief component of ECM, HPSE is not expressed in normal epithelial cells but can be detected in a variety of human carcinomas including pancreatic cancer. In the present study, human pancreatic cancer cell line Panc-1 was transfected with HPSE antisense oligodeoxynucleotide （AS-ODN） in vitro, then the inhibitory effect of ASODN on HPSE gene expression and invasive ability of Panc-1 cells in vitro was examined. The HPSE mRNA and protein expression of Panc-1 cells transfected with AS-ODN was significantly inhibited. However, there were no marked inhibitory effects in Panc-1 cells treated with nonsense oligodeoxynucleotide （NS-ODN）. Moreover, a modified Boyden chamber assay demonstrated that transfection with HPSE AS-ODN significantly inhibited invasive potential of Panc-1 cells in vitro after AS-ODN transfection. This suggests that HPSE AS-ODN may contribute to the inhibition of HPSE mRNA and protein expression, and results in a decrease of the invasive ability of Panc-1 in vitro.</description><subject>Cell Line, Tumor</subject><subject>Extracellular Matrix - metabolism</subject><subject>Glucuronidase - biosynthesis</subject><subject>Glucuronidase - genetics</subject><subject>Humans</subject><subject>Neoplasm Invasiveness</subject><subject>Oligonucleotides, Antisense - genetics</subject><subject>Oligonucleotides, Antisense - pharmacology</subject><subject>Pancreatic Neoplasms - enzymology</subject><subject>Pancreatic Neoplasms - genetics</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>基因表达</subject><subject>基因转染</subject><subject>病理机制</subject><subject>胰腺肿瘤</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cFuGyEQANBV1apJ0176ARXqoZdo2wGWZTlaVhpHipQe0vOKhcHBWYMDu679F_3kEDk99wIjzWNm0FTVZwrfKYD8MThgHeugYW-qc6oUrykX7G2JW8lqkJyfVR9y3gAI2bLmfXVWEpQCZefV3_ukQ3ZoJh8DiY4swuQzhozkbvTraDEejmE2I8bJWyQ34cEPfspkhTtdnuoCrzEguTrsEub8UkUHW9xeZ79Hshj86KfjS-nVvNWB_NLBJNSTN2RZQkxkieNIfCB7P6X4sXrn9Jjx0-t9Uf3-eXW_XNW3d9c3y8VtbRjtoBZN17GWKS01llMAE65zSilqLLaiUWIov4WGF-QMZ0BRCmuEtLrkrOYX1eWp7h8dnA7rfhPnFErHftocH-3hMPTIAFqgwKDobye9S_Fpxjz1W59NmVsHjHPuW6kUA9X9F1IlOykaUeCXVzgPW7T9LvmtTsf-32oK-HoC5iGG9ZMvIw7aPDo_Ys_KginnjD8DUx2bpQ</recordid><startdate>2006</startdate><enddate>2006</enddate><creator>高军 苏琳 秦仁义 常青 黄涛 冯延平</creator><general>Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China%Department of Internal Medicine, Zichuan Hospital, Zibo 255100, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2006</creationdate><title>Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro</title><author>高军 苏琳 秦仁义 常青 黄涛 冯延平</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2180-54882629a7ae29a5025f8f9991cde65495b057043262fc3201e75dc57da495da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cell Line, Tumor</topic><topic>Extracellular Matrix - metabolism</topic><topic>Glucuronidase - biosynthesis</topic><topic>Glucuronidase - genetics</topic><topic>Humans</topic><topic>Neoplasm Invasiveness</topic><topic>Oligonucleotides, Antisense - genetics</topic><topic>Oligonucleotides, Antisense - pharmacology</topic><topic>Pancreatic Neoplasms - enzymology</topic><topic>Pancreatic Neoplasms - genetics</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>基因表达</topic><topic>基因转染</topic><topic>病理机制</topic><topic>胰腺肿瘤</topic><toplevel>online_resources</toplevel><creatorcontrib>高军 苏琳 秦仁义 常青 黄涛 冯延平</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>高军 苏琳 秦仁义 常青 黄涛 冯延平</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2006</date><risdate>2006</risdate><volume>26</volume><issue>1</issue><spage>72</spage><epage>74</epage><pages>72-74</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Extracellular matrix （ECM） degradation is an essential step that allows tumor cells to penetrate a tissue barrier and become metastatic. 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| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2006, Vol.26 (1), p.72-74 |
| issn | 1672-0733 1993-1352 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Cell Line, Tumor Extracellular Matrix - metabolism Glucuronidase - biosynthesis Glucuronidase - genetics Humans Neoplasm Invasiveness Oligonucleotides, Antisense - genetics Oligonucleotides, Antisense - pharmacology Pancreatic Neoplasms - enzymology Pancreatic Neoplasms - genetics Pancreatic Neoplasms - pathology RNA, Messenger - biosynthesis RNA, Messenger - genetics 基因表达 基因转染 病理机制 胰腺肿瘤 |
| title | Transfection of Antisense Oligodeoxynucleotide Inhibits Heparanase Gene Expression and Invasive Ability of Human Pancreatic Cancer Cell in vitro |
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