Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells
Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2005, Vol.25 (5), p.575-577 |
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| container_title | Journal of Huazhong University of Science and Technology. Medical sciences |
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| creator | 李新宇 李中国 邱良秀 赵长松 胡竹林 |
| description | Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different. |
| doi_str_mv | 10.1007/bf02896022 |
| format | Article |
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The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/bf02896022</identifier><identifier>PMID: 16463679</identifier><language>eng</language><publisher>China: Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</publisher><subject>Animals ; Cell Proliferation - drug effects ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelium, Corneal - cytology ; Epithelium, Corneal - cytology ; Nerve Growth Factor - pharmacology ; Rabbits ; 上皮细胞 ; 动物实验 ; 神经生长因子 ; 细胞增殖 ; 角膜损伤</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (5), p.575-577</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2642-cdc717dce3f1fbaa15bcac6e1d4c2e4f8639db1052a2d81d0bf14f66628c67733</citedby><cites>FETCH-LOGICAL-c2642-cdc717dce3f1fbaa15bcac6e1d4c2e4f8639db1052a2d81d0bf14f66628c67733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16463679$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>李新宇 李中国 邱良秀 赵长松 胡竹林</creatorcontrib><title>Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.</description><subject>Animals</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelium, Corneal - cytology</subject><subject>Epithelium, Corneal - cytology</subject><subject>Nerve Growth Factor - pharmacology</subject><subject>Rabbits</subject><subject>上皮细胞</subject><subject>动物实验</subject><subject>神经生长因子</subject><subject>细胞增殖</subject><subject>角膜损伤</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1vEzEQhi0EoqVw4QcgiwsSaMH2buzssaySglQ-hOBs-WPcOHXs1PbS9t_jKkEIzWFGo0fvvDOD0EtK3lNCxAftCFuOnDD2CJ3Scew72i_Y41ZzwToi-v4EPStlS8hCcDY8RSeUD7znYjxF4Svk34AvcrqtG7xWpqaMvyQ7B1UBf88peAdZVZ8iTg5Pc6hzBot_KK19xVPKEVTAq2hT3UDwrZ4ghIJVtHi19_81n6MnToUCL475DP1ar35On7rLbxefp_PLzjA-sM5YI6iwBnpHnVaKLrRRhgO1g2EwuCXvR6spWTDF7JJaoh0dHOecLQ0Xbd0z9O6ge6uiU_FKbtOcY5so6_b-2t7daQmsXaNFzxr95kDvc7qZoVS588U0vypCmotsguMoRvpAvj2QJqdSMji5z36n8r2kRD68Qn5c_31Fg18dZWe9A_sPPd6-Aa-PapsUr25886mVuXY-gGTNGqdtlz9TO4-t</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>李新宇 李中国 邱良秀 赵长松 胡竹林</creator><general>Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells</title><author>李新宇 李中国 邱良秀 赵长松 胡竹林</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2642-cdc717dce3f1fbaa15bcac6e1d4c2e4f8639db1052a2d81d0bf14f66628c67733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelium, Corneal - cytology</topic><topic>Epithelium, Corneal - cytology</topic><topic>Nerve Growth Factor - pharmacology</topic><topic>Rabbits</topic><topic>上皮细胞</topic><topic>动物实验</topic><topic>神经生长因子</topic><topic>细胞增殖</topic><topic>角膜损伤</topic><toplevel>online_resources</toplevel><creatorcontrib>李新宇 李中国 邱良秀 赵长松 胡竹林</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>李新宇 李中国 邱良秀 赵长松 胡竹林</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2005</date><risdate>2005</risdate><volume>25</volume><issue>5</issue><spage>575</spage><epage>577</epage><pages>575-577</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Summary： In order to investigate the effect of nerve growth factor （NGF） on the proliferation of rabbit corneal endothelial cells and epithelial cells, the in vitro cultured rabbit corneal endothelial cells and epithelial cells were treated with different concentrations of NGF.MTT assay was used to examine the clonal growth and proliferation of the cells by determining the absorbency values at 570 nm. The results showed that NGF with three concentrations ranging from 5 U/mL to 500 U/mL enhanced the proliferation of rabbit corneal endothelial cells in a concentration-dependent manner. 50 U/mI. and 500 U/mI. NGF got more increase of proliferation than that of 5 U/mL NGF did. Meanwhile, 50 U/mL and 500 U/mL NGF could promote the proliferation of the rabbit corneal epithelial cells significantly in a concentration-dependent manner. However, 5 U/mL NGF did not enhance the proliferation of epithelial cells. It was suggested that exogenous NGF can stimulate the proliferation of both rabbit corneal endothelial and epithelial cells, but the extent of modulation is different.</abstract><cop>China</cop><pub>Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</pub><pmid>16463679</pmid><doi>10.1007/bf02896022</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (5), p.575-577 |
| issn | 1672-0733 1993-1352 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Proliferation - drug effects Cells, Cultured Dose-Response Relationship, Drug Endothelium, Corneal - cytology Epithelium, Corneal - cytology Nerve Growth Factor - pharmacology Rabbits 上皮细胞 动物实验 神经生长因子 细胞增殖 角膜损伤 |
| title | Nerve Growth Factor Modulate Proliferation of Cultured Rabbit Corneal Endothelial Cells and Epithelial Cells |
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