Hypoxia Down-regulates Secretion of MMP-2, MMP-9 in Porcine Pulmonary Artery Endothelial and Smooth Muscle Cells and the Role of HIF-1
Summary： Primary cell culture, techniques of gene transfection, gelatin zymography, and Western blot were used to investigate the effect of hypoxia on the secretion of MMP 2 and MMP-9 in pulmonary artery endothelial cells （PAEC） and smooth muscle cells （PASMC）, and the role of HIF-1. Our results sho...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2005, Vol.25 (4), p.382-384 |
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| creator | 叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔 |
| description | Summary： Primary cell culture, techniques of gene transfection, gelatin zymography, and Western blot were used to investigate the effect of hypoxia on the secretion of MMP 2 and MMP-9 in pulmonary artery endothelial cells （PAEC） and smooth muscle cells （PASMC）, and the role of HIF-1. Our results showed that （1） after exposure to hypoxia for 24 h, the protein content and activity of MMP-2 in the PAEC medium as well as these of MMP-2 and MMP-9 in PASMC medium （P〈0. 01 ） decreased significantly in contrast to those in normoxic group （P（0.05） ; （2） after transfection of wild type EPO3＇ enhancer, a HIF-1 decoy, the content and activity of MMP 2 and MMP-9 in hypoxic mediums became higher than those in normoxic group （P〈0. 01）, while transfection of mutant EPO3＇-enhancer didn＇t affect the hypoxia-induced down-regulation. It is concluded that hypoxia could inhibit the secretion and activity of MMP 2 and MMP-9 in PAEC and PASMC, which could he mitigated by the transfection of EPO3 ＇-enhancer and that H1F-1 pathway might contribute to hypoxia-induced down regulation of MMP-2 and MMP-9. |
| doi_str_mv | 10.1007/bf02828202 |
| format | Article |
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Our results showed that （1） after exposure to hypoxia for 24 h, the protein content and activity of MMP-2 in the PAEC medium as well as these of MMP-2 and MMP-9 in PASMC medium （P〈0. 01 ） decreased significantly in contrast to those in normoxic group （P（0.05） ; （2） after transfection of wild type EPO3＇ enhancer, a HIF-1 decoy, the content and activity of MMP 2 and MMP-9 in hypoxic mediums became higher than those in normoxic group （P〈0. 01）, while transfection of mutant EPO3＇-enhancer didn＇t affect the hypoxia-induced down-regulation. It is concluded that hypoxia could inhibit the secretion and activity of MMP 2 and MMP-9 in PAEC and PASMC, which could he mitigated by the transfection of EPO3 ＇-enhancer and that H1F-1 pathway might contribute to hypoxia-induced down regulation of MMP-2 and MMP-9.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/bf02828202</identifier><identifier>PMID: 16196282</identifier><language>eng</language><publisher>China: Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Pulmonary Laboratory of Ministry of Health of China, Wuhan 430030, China%Department of Preventive Medicine, Xinyang Vocational and Technical School, Xinyang 464000, China%Department of Respiratory Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</publisher><subject>Animals ; Cell Hypoxia ; Cells, Cultured ; Down-Regulation ; Endothelial Cells - cytology ; Endothelial Cells - enzymology ; Enhancer Elements, Genetic ; Erythropoietin - genetics ; Erythropoietin - pharmacology ; Hypoxia-Inducible Factor 1 - metabolism ; Matrix Metalloproteinase 2 - secretion ; Matrix Metalloproteinase 9 - secretion ; MMP-2 ; MMP-9 ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - enzymology ; Pulmonary Artery - cytology ; Pulmonary Artery - enzymology ; Swine ; 动物实验 ; 平滑肌细胞 ; 组织缺氧 ; 肺动脉疾病</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (4), p.382-384</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2944-6c668aefd7fa20dad98f2bd30814434716f61ff2187508d8c70706ee6cd0882e3</citedby><cites>FETCH-LOGICAL-c2944-6c668aefd7fa20dad98f2bd30814434716f61ff2187508d8c70706ee6cd0882e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>315,781,785,4025,27925,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16196282$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔</creatorcontrib><title>Hypoxia Down-regulates Secretion of MMP-2, MMP-9 in Porcine Pulmonary Artery Endothelial and Smooth Muscle Cells and the Role of HIF-1</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>Summary： Primary cell culture, techniques of gene transfection, gelatin zymography, and Western blot were used to investigate the effect of hypoxia on the secretion of MMP 2 and MMP-9 in pulmonary artery endothelial cells （PAEC） and smooth muscle cells （PASMC）, and the role of HIF-1. Our results showed that （1） after exposure to hypoxia for 24 h, the protein content and activity of MMP-2 in the PAEC medium as well as these of MMP-2 and MMP-9 in PASMC medium （P〈0. 01 ） decreased significantly in contrast to those in normoxic group （P（0.05） ; （2） after transfection of wild type EPO3＇ enhancer, a HIF-1 decoy, the content and activity of MMP 2 and MMP-9 in hypoxic mediums became higher than those in normoxic group （P〈0. 01）, while transfection of mutant EPO3＇-enhancer didn＇t affect the hypoxia-induced down-regulation. It is concluded that hypoxia could inhibit the secretion and activity of MMP 2 and MMP-9 in PAEC and PASMC, which could he mitigated by the transfection of EPO3 ＇-enhancer and that H1F-1 pathway might contribute to hypoxia-induced down regulation of MMP-2 and MMP-9.</description><subject>Animals</subject><subject>Cell Hypoxia</subject><subject>Cells, Cultured</subject><subject>Down-Regulation</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - enzymology</subject><subject>Enhancer Elements, Genetic</subject><subject>Erythropoietin - genetics</subject><subject>Erythropoietin - pharmacology</subject><subject>Hypoxia-Inducible Factor 1 - metabolism</subject><subject>Matrix Metalloproteinase 2 - secretion</subject><subject>Matrix Metalloproteinase 9 - secretion</subject><subject>MMP-2</subject><subject>MMP-9</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - enzymology</subject><subject>Pulmonary Artery - cytology</subject><subject>Pulmonary Artery - enzymology</subject><subject>Swine</subject><subject>动物实验</subject><subject>平滑肌细胞</subject><subject>组织缺氧</subject><subject>肺动脉疾病</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1uEzEUhUcIREthwwMgi0UXwIB_ZmzPsoSGVGpERGFteezrdNIZO7Vn1OQFeG5ME8QSeXHtq8_n6txTFK8J_kgwFp9ah6nMB9MnxSlpGlYSVtOn-c4FLbFg7KR4kdIG41pwWj0vTggnDc8_Totfi_027DqNvoQHX0ZYT70eIaEbMBHGLngUHFouVyX98Fga1Hm0CtF0HtBq6ofgddyjizhCLpfehvEW-k73SHuLboaQ32g5JdMDmkHfp8d-ZtD3kFtZfHE1L8nL4pnTfYJXx3pW_Jxf_pgtyutvX69mF9eloU1VldxwLjU4K5ym2GrbSEdby7AkVcUqQbjjxDlKpKixtNIILDAH4MZiKSmws-L9QfdBe6f9Wm3CFH2eqMbN_s7udq0CmveEK4yrTJ8f6G0M9xOkUQ1dMtmF9hCmpLjkTNKm_i9IBJO8oiyD7w6giSGlCE5tYzfkDSqC1Z8w1ef53zAz_OaoOrUD2H_oMb0MvD2q3Qa_vu-yn1abO9f1oLILITGv2W-uRqKH</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔</creator><general>Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Pulmonary Laboratory of Ministry of Health of China, Wuhan 430030, China%Department of Preventive Medicine, Xinyang Vocational and Technical School, Xinyang 464000, China%Department of Respiratory Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>Hypoxia Down-regulates Secretion of MMP-2, MMP-9 in Porcine Pulmonary Artery Endothelial and Smooth Muscle Cells and the Role of HIF-1</title><author>叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2944-6c668aefd7fa20dad98f2bd30814434716f61ff2187508d8c70706ee6cd0882e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Cell Hypoxia</topic><topic>Cells, Cultured</topic><topic>Down-Regulation</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - enzymology</topic><topic>Enhancer Elements, Genetic</topic><topic>Erythropoietin - genetics</topic><topic>Erythropoietin - pharmacology</topic><topic>Hypoxia-Inducible Factor 1 - metabolism</topic><topic>Matrix Metalloproteinase 2 - secretion</topic><topic>Matrix Metalloproteinase 9 - secretion</topic><topic>MMP-2</topic><topic>MMP-9</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - enzymology</topic><topic>Pulmonary Artery - cytology</topic><topic>Pulmonary Artery - enzymology</topic><topic>Swine</topic><topic>动物实验</topic><topic>平滑肌细胞</topic><topic>组织缺氧</topic><topic>肺动脉疾病</topic><toplevel>online_resources</toplevel><creatorcontrib>叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>叶红 郑延芳 马万里 柯丹 金咸瑢 刘声远 王迪浔</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypoxia Down-regulates Secretion of MMP-2, MMP-9 in Porcine Pulmonary Artery Endothelial and Smooth Muscle Cells and the Role of HIF-1</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2005</date><risdate>2005</risdate><volume>25</volume><issue>4</issue><spage>382</spage><epage>384</epage><pages>382-384</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>Summary： Primary cell culture, techniques of gene transfection, gelatin zymography, and Western blot were used to investigate the effect of hypoxia on the secretion of MMP 2 and MMP-9 in pulmonary artery endothelial cells （PAEC） and smooth muscle cells （PASMC）, and the role of HIF-1. Our results showed that （1） after exposure to hypoxia for 24 h, the protein content and activity of MMP-2 in the PAEC medium as well as these of MMP-2 and MMP-9 in PASMC medium （P〈0. 01 ） decreased significantly in contrast to those in normoxic group （P（0.05） ; （2） after transfection of wild type EPO3＇ enhancer, a HIF-1 decoy, the content and activity of MMP 2 and MMP-9 in hypoxic mediums became higher than those in normoxic group （P〈0. 01）, while transfection of mutant EPO3＇-enhancer didn＇t affect the hypoxia-induced down-regulation. It is concluded that hypoxia could inhibit the secretion and activity of MMP 2 and MMP-9 in PAEC and PASMC, which could he mitigated by the transfection of EPO3 ＇-enhancer and that H1F-1 pathway might contribute to hypoxia-induced down regulation of MMP-2 and MMP-9.</abstract><cop>China</cop><pub>Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Pulmonary Laboratory of Ministry of Health of China, Wuhan 430030, China%Department of Preventive Medicine, Xinyang Vocational and Technical School, Xinyang 464000, China%Department of Respiratory Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China</pub><pmid>16196282</pmid><doi>10.1007/bf02828202</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (4), p.382-384 |
| issn | 1672-0733 1993-1352 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Hypoxia Cells, Cultured Down-Regulation Endothelial Cells - cytology Endothelial Cells - enzymology Enhancer Elements, Genetic Erythropoietin - genetics Erythropoietin - pharmacology Hypoxia-Inducible Factor 1 - metabolism Matrix Metalloproteinase 2 - secretion Matrix Metalloproteinase 9 - secretion MMP-2 MMP-9 Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - enzymology Pulmonary Artery - cytology Pulmonary Artery - enzymology Swine 动物实验 平滑肌细胞 组织缺氧 肺动脉疾病 |
| title | Hypoxia Down-regulates Secretion of MMP-2, MMP-9 in Porcine Pulmonary Artery Endothelial and Smooth Muscle Cells and the Role of HIF-1 |
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