Effect of Angiotensin Ⅱ on Cord Blood CD34^＋ Cells Expansion in vitro
In order to investigate the influence of angiotensin Ⅱ on hematopoietic system, CD34^+ cells in cord blood were purified, and the effects of angiotensin Ⅱ in combination with various cytokines on their growth and differentiation were studied by cell culture in vitro. It was found that angiotensin Ⅱ...
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| Veröffentlicht in: | Journal of Huazhong University of Science and Technology. Medical sciences 2005, Vol.25 (1), p.26-28 |
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| creator | 彭程 邹萍 马艳萍 胡中波 |
| description | In order to investigate the influence of angiotensin Ⅱ on hematopoietic system, CD34^+ cells in cord blood were purified, and the effects of angiotensin Ⅱ in combination with various cytokines on their growth and differentiation were studied by cell culture in vitro. It was found that angiotensin Ⅱ in suspending medium could stimulate both BFU-E and CFU-GM expansion. The number of BFU-E and CFU-GM was increased with the increases of angiotensin Ⅱ concentrations during a certain range. In addition, the expansion fold of CFU-GM was increased from 2.3~0.8 times to 7.8±2.3 times when angiotensin Ⅱ was added in the presence of SCF+G-CSF+GM-CSF+IL-3 cytokines mixture. Similarly, the expansion fold of BFU-E was increased from 3.1±1.8 times to 9.2±2.3 times with angiotensin Ⅱ in the presence of SCF+EPO+TPO+IL-3. In the semi-solid medium, angiotensin Ⅱ could stimulate CFU-GM expansion but had no effect on the growth of BFU-E. In conclusion, angiotensin Ⅱ had some stimulating effects on cord blood hematopoietic progenitors expansion in vitro in the presence of other cytokines. |
| doi_str_mv | 10.1007/bf02831378 |
| format | Article |
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It was found that angiotensin Ⅱ in suspending medium could stimulate both BFU-E and CFU-GM expansion. The number of BFU-E and CFU-GM was increased with the increases of angiotensin Ⅱ concentrations during a certain range. In addition, the expansion fold of CFU-GM was increased from 2.3~0.8 times to 7.8±2.3 times when angiotensin Ⅱ was added in the presence of SCF+G-CSF+GM-CSF+IL-3 cytokines mixture. Similarly, the expansion fold of BFU-E was increased from 3.1±1.8 times to 9.2±2.3 times with angiotensin Ⅱ in the presence of SCF+EPO+TPO+IL-3. In the semi-solid medium, angiotensin Ⅱ could stimulate CFU-GM expansion but had no effect on the growth of BFU-E. In conclusion, angiotensin Ⅱ had some stimulating effects on cord blood hematopoietic progenitors expansion in vitro in the presence of other cytokines.</description><identifier>ISSN: 1672-0733</identifier><identifier>EISSN: 1993-1352</identifier><identifier>DOI: 10.1007/bf02831378</identifier><identifier>PMID: 15934300</identifier><language>eng</language><publisher>China: Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China</publisher><subject>Angiotensin II - pharmacology ; Antigens, CD34 - metabolism ; BFU-E ; CD34 ; Cells, Cultured ; CFU-GM ; Colony-Forming Units Assay ; EPO ; Fetal Blood - cytology ; G-CSF ; GM-CSF ; Granulocyte-Macrophage Colony-Stimulating Factor ; Hematopoietic Stem Cells - cytology ; Humans ; IL-3 ; SCF ; TPO</subject><ispartof>Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (1), p.26-28</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15934300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>彭程 邹萍 马艳萍 胡中波</creatorcontrib><title>Effect of Angiotensin Ⅱ on Cord Blood CD34^＋ Cells Expansion in vitro</title><title>Journal of Huazhong University of Science and Technology. Medical sciences</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>In order to investigate the influence of angiotensin Ⅱ on hematopoietic system, CD34^+ cells in cord blood were purified, and the effects of angiotensin Ⅱ in combination with various cytokines on their growth and differentiation were studied by cell culture in vitro. It was found that angiotensin Ⅱ in suspending medium could stimulate both BFU-E and CFU-GM expansion. The number of BFU-E and CFU-GM was increased with the increases of angiotensin Ⅱ concentrations during a certain range. In addition, the expansion fold of CFU-GM was increased from 2.3~0.8 times to 7.8±2.3 times when angiotensin Ⅱ was added in the presence of SCF+G-CSF+GM-CSF+IL-3 cytokines mixture. Similarly, the expansion fold of BFU-E was increased from 3.1±1.8 times to 9.2±2.3 times with angiotensin Ⅱ in the presence of SCF+EPO+TPO+IL-3. In the semi-solid medium, angiotensin Ⅱ could stimulate CFU-GM expansion but had no effect on the growth of BFU-E. In conclusion, angiotensin Ⅱ had some stimulating effects on cord blood hematopoietic progenitors expansion in vitro in the presence of other cytokines.</description><subject>Angiotensin II - pharmacology</subject><subject>Antigens, CD34 - metabolism</subject><subject>BFU-E</subject><subject>CD34</subject><subject>Cells, Cultured</subject><subject>CFU-GM</subject><subject>Colony-Forming Units Assay</subject><subject>EPO</subject><subject>Fetal Blood - cytology</subject><subject>G-CSF</subject><subject>GM-CSF</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor</subject><subject>Hematopoietic Stem Cells - cytology</subject><subject>Humans</subject><subject>IL-3</subject><subject>SCF</subject><subject>TPO</subject><issn>1672-0733</issn><issn>1993-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10EtOwzAQBmALgXhvOACyWLBBAT8S21lCKFAJiQ1sicaOXVJSuyQptAdgxSm4B6fhAlwBo5bVzOLT_PoHoQNKTikh8kw7whSnXKo1tE3znCeUZ2w97kKyhEjOt9BO140JyaRg6SbaolnOU07INhoOnLOmx8Hhcz-qQ299V3v8_f6Jg8dFaCt80YRQ4eKSp48_Xx-4sE3T4cF8ClFGE_Vr3bdhD204aDq7v5q76OFqcF_cJLd318Pi_DYxVEmVaMFzcE4wrZ1hDpgGSrigXIicVNYaKbJKVoJxkEYpS7nTAGBSAQoyCXwXnSzvvoF34EflOMxaHxPLfrx4ruZzXVoWq5L4GxX18VJP2_Ays11fTurOxArgbZh1pZAqz1LxBw9XcKYntiqnbT2BdlH-vyqCoyUwT8GPXuqYrME8u7qxEVHBZSzxC9F3dg4</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>彭程 邹萍 马艳萍 胡中波</creator><general>Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2005</creationdate><title>Effect of Angiotensin Ⅱ on Cord Blood CD34^＋ Cells Expansion in vitro</title><author>彭程 邹萍 马艳萍 胡中波</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1878-b639aff62bbfc2fa2ba1036136690deec765d7d623a7c88e13fbaaac46a8a57a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Antigens, CD34 - metabolism</topic><topic>BFU-E</topic><topic>CD34</topic><topic>Cells, Cultured</topic><topic>CFU-GM</topic><topic>Colony-Forming Units Assay</topic><topic>EPO</topic><topic>Fetal Blood - cytology</topic><topic>G-CSF</topic><topic>GM-CSF</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor</topic><topic>Hematopoietic Stem Cells - cytology</topic><topic>Humans</topic><topic>IL-3</topic><topic>SCF</topic><topic>TPO</topic><toplevel>online_resources</toplevel><creatorcontrib>彭程 邹萍 马艳萍 胡中波</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>彭程 邹萍 马艳萍 胡中波</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Angiotensin Ⅱ on Cord Blood CD34^＋ Cells Expansion in vitro</atitle><jtitle>Journal of Huazhong University of Science and Technology. Medical sciences</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2005</date><risdate>2005</risdate><volume>25</volume><issue>1</issue><spage>26</spage><epage>28</epage><pages>26-28</pages><issn>1672-0733</issn><eissn>1993-1352</eissn><abstract>In order to investigate the influence of angiotensin Ⅱ on hematopoietic system, CD34^+ cells in cord blood were purified, and the effects of angiotensin Ⅱ in combination with various cytokines on their growth and differentiation were studied by cell culture in vitro. It was found that angiotensin Ⅱ in suspending medium could stimulate both BFU-E and CFU-GM expansion. The number of BFU-E and CFU-GM was increased with the increases of angiotensin Ⅱ concentrations during a certain range. In addition, the expansion fold of CFU-GM was increased from 2.3~0.8 times to 7.8±2.3 times when angiotensin Ⅱ was added in the presence of SCF+G-CSF+GM-CSF+IL-3 cytokines mixture. Similarly, the expansion fold of BFU-E was increased from 3.1±1.8 times to 9.2±2.3 times with angiotensin Ⅱ in the presence of SCF+EPO+TPO+IL-3. In the semi-solid medium, angiotensin Ⅱ could stimulate CFU-GM expansion but had no effect on the growth of BFU-E. In conclusion, angiotensin Ⅱ had some stimulating effects on cord blood hematopoietic progenitors expansion in vitro in the presence of other cytokines.</abstract><cop>China</cop><pub>Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, China</pub><pmid>15934300</pmid><doi>10.1007/bf02831378</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0733 |
| ispartof | Journal of Huazhong University of Science and Technology. Medical sciences, 2005, Vol.25 (1), p.26-28 |
| issn | 1672-0733 1993-1352 |
| language | eng |
| recordid | cdi_wanfang_journals_tjykdxxb_e200501008 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Angiotensin II - pharmacology Antigens, CD34 - metabolism BFU-E CD34 Cells, Cultured CFU-GM Colony-Forming Units Assay EPO Fetal Blood - cytology G-CSF GM-CSF Granulocyte-Macrophage Colony-Stimulating Factor Hematopoietic Stem Cells - cytology Humans IL-3 SCF TPO |
| title | Effect of Angiotensin Ⅱ on Cord Blood CD34^＋ Cells Expansion in vitro |
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