Experimental Study of Vascular Endothelial Growth Factor Gene Therapy for Avascular Necrosis of the Femoral Head
To explore a new method for the therapy of the avascular necrosis of the femoral head,the recombinant plasmid pCD-hVEGF16s was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and imm...
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| Veröffentlicht in: | Current medical science 2003, Vol.23 (3), p.297-299 |
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| creator | 杨操 杨述华 杜靖远 李进 许伟华 熊宇芳 |
| description | To explore a new method for the therapy of the avascular necrosis of the femoral head,the recombinant plasmid pCD-hVEGF16s was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly. |
| doi_str_mv | 10.1007/bf02829520 |
| format | Article |
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The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly.</description><identifier>ISSN: 1672-0733</identifier><identifier>ISSN: 2096-5230</identifier><identifier>EISSN: 2523-899X</identifier><identifier>DOI: 10.1007/bf02829520</identifier><identifier>PMID: 14526440</identifier><language>eng</language><publisher>China: Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technolog y,Wuhan 430022%Department of Biochemistry, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030</publisher><subject>Animals ; Collagen - therapeutic use ; Femur Head - blood supply ; Femur Head Necrosis - therapy ; Gene Transfer Techniques ; Genetic Therapy ; Implants, Experimental ; Neovascularization, Physiologic ; Osteogenesis ; Plasmids - genetics ; Rabbits ; Random Allocation ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Vascular Endothelial Growth Factor A - biosynthesis ; Vascular Endothelial Growth Factor A - genetics ; 临床疗效 ; 基因治疗 ; 股骨头坏死 ; 血管内皮生长因子</subject><ispartof>Current medical science, 2003, Vol.23 (3), p.297-299</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2607-16df6eb6fbcc0e2aee40acacd2a0052a7cf4eb6dbe6fc9fc5b8b1a41a97ca1f73</citedby><cites>FETCH-LOGICAL-c2607-16df6eb6fbcc0e2aee40acacd2a0052a7cf4eb6dbe6fc9fc5b8b1a41a97ca1f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85740A/85740A.jpg</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14526440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>杨操 杨述华 杜靖远 李进 许伟华 熊宇芳</creatorcontrib><title>Experimental Study of Vascular Endothelial Growth Factor Gene Therapy for Avascular Necrosis of the Femoral Head</title><title>Current medical science</title><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><description>To explore a new method for the therapy of the avascular necrosis of the femoral head,the recombinant plasmid pCD-hVEGF16s was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly.</description><subject>Animals</subject><subject>Collagen - therapeutic use</subject><subject>Femur Head - blood supply</subject><subject>Femur Head Necrosis - therapy</subject><subject>Gene Transfer Techniques</subject><subject>Genetic Therapy</subject><subject>Implants, Experimental</subject><subject>Neovascularization, Physiologic</subject><subject>Osteogenesis</subject><subject>Plasmids - genetics</subject><subject>Rabbits</subject><subject>Random Allocation</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Vascular Endothelial Growth Factor A - biosynthesis</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>临床疗效</subject><subject>基因治疗</subject><subject>股骨头坏死</subject><subject>血管内皮生长因子</subject><issn>1672-0733</issn><issn>2096-5230</issn><issn>2523-899X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkVFv0zAQgC3ExLrBCz8AmRcemAK248TJ45jabtIEDwzEm3V2zmu6NM7shLX_fq7aafKDJd933_nuCPnI2TfOmPpuHBOVqAvB3pCZKESeVXX97y2Z8VKJjKk8PyVnMa4ZK1Qp5DtyymUhSinZjAzz7YCh3WA_Qkd_j1Ozo97RvxDt1EGg877x4wq7NkWXwT-NK7oAO_pAl9gjvVthgGFHXXq4_P-S9BNt8LGNe1NKpgvc-JAE1wjNe3LioIv44Xifkz-L-d3VdXb7a3lzdXmbWVEylfGycSWa0hlrGQpAlAws2EZAakOAsk6mcGOwdLZ2tjCV4SA51MoCdyo_JxcH7xP0Dvp7vfZT6FNFPa53D812azQKxvJ0xJ7-cqCH4B8njKPetNFi10GPfopaFUrmklcJ_HoA9x3GgE4PaXoQdpozvd-G_rF42UaCPx2tk9lg84oex5-Az0fbyvf3j236pgH74NoOdSWZkqrOnwEfWZIb</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>杨操 杨述华 杜靖远 李进 许伟华 熊宇芳</creator><general>Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technolog y,Wuhan 430022%Department of Biochemistry, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>2003</creationdate><title>Experimental Study of Vascular Endothelial Growth Factor Gene Therapy for Avascular Necrosis of the Femoral Head</title><author>杨操 杨述华 杜靖远 李进 许伟华 熊宇芳</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2607-16df6eb6fbcc0e2aee40acacd2a0052a7cf4eb6dbe6fc9fc5b8b1a41a97ca1f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Collagen - therapeutic use</topic><topic>Femur Head - blood supply</topic><topic>Femur Head Necrosis - therapy</topic><topic>Gene Transfer Techniques</topic><topic>Genetic Therapy</topic><topic>Implants, Experimental</topic><topic>Neovascularization, Physiologic</topic><topic>Osteogenesis</topic><topic>Plasmids - genetics</topic><topic>Rabbits</topic><topic>Random Allocation</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - genetics</topic><topic>Vascular Endothelial Growth Factor A - biosynthesis</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>临床疗效</topic><topic>基因治疗</topic><topic>股骨头坏死</topic><topic>血管内皮生长因子</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>杨操 杨述华 杜靖远 李进 许伟华 熊宇芳</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Current medical science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>杨操 杨述华 杜靖远 李进 许伟华 熊宇芳</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Experimental Study of Vascular Endothelial Growth Factor Gene Therapy for Avascular Necrosis of the Femoral Head</atitle><jtitle>Current medical science</jtitle><addtitle>Journal of Zuazhong University of Science and Technology: Medical Edition</addtitle><date>2003</date><risdate>2003</risdate><volume>23</volume><issue>3</issue><spage>297</spage><epage>299</epage><pages>297-299</pages><issn>1672-0733</issn><issn>2096-5230</issn><eissn>2523-899X</eissn><abstract>To explore a new method for the therapy of the avascular necrosis of the femoral head,the recombinant plasmid pCD-hVEGF16s was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was detected by RNA dot hybridization and immunohistochemical method. The repair of the femoral head was observed by histological method. The results showed that the expression of VEGF was detectable in the femoral head treated with VEGF gene. Angiogenesis in these femoral heads was more abundant than the control. Bone repairing was augmented in the femoral head treated with VEGF gene. The results suggest that angiogenesis in bone tissue could be augmented by gene transfection of VEGF and bone repairing would be accelerated accordingly.</abstract><cop>China</cop><pub>Department of Orthopedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technolog y,Wuhan 430022%Department of Biochemistry, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030</pub><pmid>14526440</pmid><doi>10.1007/bf02829520</doi><tpages>3</tpages></addata></record> |
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| ispartof | Current medical science, 2003, Vol.23 (3), p.297-299 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Alma/SFX Local Collection |
| subjects | Animals Collagen - therapeutic use Femur Head - blood supply Femur Head Necrosis - therapy Gene Transfer Techniques Genetic Therapy Implants, Experimental Neovascularization, Physiologic Osteogenesis Plasmids - genetics Rabbits Random Allocation Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Vascular Endothelial Growth Factor A - biosynthesis Vascular Endothelial Growth Factor A - genetics 临床疗效 基因治疗 股骨头坏死 血管内皮生长因子 |
| title | Experimental Study of Vascular Endothelial Growth Factor Gene Therapy for Avascular Necrosis of the Femoral Head |
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