Engineering Corynebacterium glutamicum for Geraniol Production
Geraniol is a monoterpenoid alcohol with various applications in food, cosmetics, and healthcare. Corynebacterium glutamicum is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway. To engineer C . glutamicum to produce geraniol, two different trun...
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| Veröffentlicht in: | Transactions of Tianjin University 2021-10, Vol.27 (5), p.377-384 |
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| creator | Li, Man Xu, Shuo Lu, Wenyu |
| description | Geraniol is a monoterpenoid alcohol with various applications in food, cosmetics, and healthcare.
Corynebacterium glutamicum
is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway. To engineer
C
.
glutamicum
to produce geraniol, two different truncated geraniol synthases (GESs) were respectively expressed, and strain LX02 expressing the truncated GESs from
Valeriana officinalis
(tVoGES) produced 0.3 mg/L of geraniol. Then, three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with tVoGES to improve geraniol production. The amounts of produced geraniol were all higher than that produced by strain LX02. Strain LX03 co-expressing ERG20
F96W–N127W
(ERG20
WW
) and tVoGES produced the highest amount, 5.4 mg/L. Subsequently, the co-overexpression of 1-deoxy-
d
-xylulose-5-phosphate synthase (
dxs
) and isopentenyl diphosphate isomerase (
idi
) further increased the production to 12.2 mg/L in strain LX03. Lastly, the production of geraniol was increased to 15.2 mg/L via fermentation optimization. To our knowledge, this is the first report on the engineering of
C. glutamicum
to produce geraniol and thus can serve as a reference for other monoterpenoid production studies. |
| doi_str_mv | 10.1007/s12209-020-00252-w |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_tianjdxxb_e202105003</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>tianjdxxb_e202105003</wanfj_id><sourcerecordid>tianjdxxb_e202105003</sourcerecordid><originalsourceid>FETCH-LOGICAL-c356t-def7565b35efd47165beec63beac98dec053aa1ab67a03edaf9eb6e8be65a7203</originalsourceid><addsrcrecordid>eNp9kEFLwzAUx4MoOKdfwFPBq9GXpEmbiyBjTmGgBz2HtH0tHVsy05Zt397MCrt5ev8Hv_978CPklsEDA8geO8Y5aAocKACXnO7OyIRpLWnOtDqPGUDRVOf8klx13Qog1ZCxCXmau6Z1iKF1TTLz4eCwsGUf92GTNOuht5u2jLH2IVlgsK716-Qj-Goo-9a7a3JR23WHN39zSr5e5p-zV7p8X7zNnpe0FFL1tMI6k0oWQmJdpRmLEbFUokBb6rzCEqSwltlCZRYEVrbWWCjMC1TSZhzElNyPd3fW1dY1ZuWH4OJH07fWrar9vjDIgTOQACLidyO-Df57wK4_8VyqVDLgqYoUH6ky-K4LWJttaDc2HAwDc9RqRq0majW_Ws0ulsRY6rZHZxhOp_9p_QBjiXya</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2564510246</pqid></control><display><type>article</type><title>Engineering Corynebacterium glutamicum for Geraniol Production</title><source>SpringerNature Journals</source><source>Alma/SFX Local Collection</source><creator>Li, Man ; Xu, Shuo ; Lu, Wenyu</creator><creatorcontrib>Li, Man ; Xu, Shuo ; Lu, Wenyu</creatorcontrib><description>Geraniol is a monoterpenoid alcohol with various applications in food, cosmetics, and healthcare.
Corynebacterium glutamicum
is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway. To engineer
C
.
glutamicum
to produce geraniol, two different truncated geraniol synthases (GESs) were respectively expressed, and strain LX02 expressing the truncated GESs from
Valeriana officinalis
(tVoGES) produced 0.3 mg/L of geraniol. Then, three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with tVoGES to improve geraniol production. The amounts of produced geraniol were all higher than that produced by strain LX02. Strain LX03 co-expressing ERG20
F96W–N127W
(ERG20
WW
) and tVoGES produced the highest amount, 5.4 mg/L. Subsequently, the co-overexpression of 1-deoxy-
d
-xylulose-5-phosphate synthase (
dxs
) and isopentenyl diphosphate isomerase (
idi
) further increased the production to 12.2 mg/L in strain LX03. Lastly, the production of geraniol was increased to 15.2 mg/L via fermentation optimization. To our knowledge, this is the first report on the engineering of
C. glutamicum
to produce geraniol and thus can serve as a reference for other monoterpenoid production studies.</description><identifier>ISSN: 1006-4982</identifier><identifier>EISSN: 1995-8196</identifier><identifier>DOI: 10.1007/s12209-020-00252-w</identifier><language>eng</language><publisher>Tianjin: Tianjin University</publisher><subject>Cosmetics ; Engineering ; Fermentation ; Harbor engineering ; Humanities and Social Sciences ; Mechanical Engineering ; multidisciplinary ; Optimization ; Research Article ; Science ; Terpenes</subject><ispartof>Transactions of Tianjin University, 2021-10, Vol.27 (5), p.377-384</ispartof><rights>Tianjin University and Springer-Verlag GmbH Germany, part of Springer Nature 2020</rights><rights>Tianjin University and Springer-Verlag GmbH Germany, part of Springer Nature 2020.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-def7565b35efd47165beec63beac98dec053aa1ab67a03edaf9eb6e8be65a7203</citedby><cites>FETCH-LOGICAL-c356t-def7565b35efd47165beec63beac98dec053aa1ab67a03edaf9eb6e8be65a7203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/tianjdxxb-e/tianjdxxb-e.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12209-020-00252-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12209-020-00252-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Li, Man</creatorcontrib><creatorcontrib>Xu, Shuo</creatorcontrib><creatorcontrib>Lu, Wenyu</creatorcontrib><title>Engineering Corynebacterium glutamicum for Geraniol Production</title><title>Transactions of Tianjin University</title><addtitle>Trans. Tianjin Univ</addtitle><description>Geraniol is a monoterpenoid alcohol with various applications in food, cosmetics, and healthcare.
Corynebacterium glutamicum
is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway. To engineer
C
.
glutamicum
to produce geraniol, two different truncated geraniol synthases (GESs) were respectively expressed, and strain LX02 expressing the truncated GESs from
Valeriana officinalis
(tVoGES) produced 0.3 mg/L of geraniol. Then, three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with tVoGES to improve geraniol production. The amounts of produced geraniol were all higher than that produced by strain LX02. Strain LX03 co-expressing ERG20
F96W–N127W
(ERG20
WW
) and tVoGES produced the highest amount, 5.4 mg/L. Subsequently, the co-overexpression of 1-deoxy-
d
-xylulose-5-phosphate synthase (
dxs
) and isopentenyl diphosphate isomerase (
idi
) further increased the production to 12.2 mg/L in strain LX03. Lastly, the production of geraniol was increased to 15.2 mg/L via fermentation optimization. To our knowledge, this is the first report on the engineering of
C. glutamicum
to produce geraniol and thus can serve as a reference for other monoterpenoid production studies.</description><subject>Cosmetics</subject><subject>Engineering</subject><subject>Fermentation</subject><subject>Harbor engineering</subject><subject>Humanities and Social Sciences</subject><subject>Mechanical Engineering</subject><subject>multidisciplinary</subject><subject>Optimization</subject><subject>Research Article</subject><subject>Science</subject><subject>Terpenes</subject><issn>1006-4982</issn><issn>1995-8196</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLwzAUx4MoOKdfwFPBq9GXpEmbiyBjTmGgBz2HtH0tHVsy05Zt397MCrt5ev8Hv_978CPklsEDA8geO8Y5aAocKACXnO7OyIRpLWnOtDqPGUDRVOf8klx13Qog1ZCxCXmau6Z1iKF1TTLz4eCwsGUf92GTNOuht5u2jLH2IVlgsK716-Qj-Goo-9a7a3JR23WHN39zSr5e5p-zV7p8X7zNnpe0FFL1tMI6k0oWQmJdpRmLEbFUokBb6rzCEqSwltlCZRYEVrbWWCjMC1TSZhzElNyPd3fW1dY1ZuWH4OJH07fWrar9vjDIgTOQACLidyO-Df57wK4_8VyqVDLgqYoUH6ky-K4LWJttaDc2HAwDc9RqRq0majW_Ws0ulsRY6rZHZxhOp_9p_QBjiXya</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Li, Man</creator><creator>Xu, Shuo</creator><creator>Lu, Wenyu</creator><general>Tianjin University</general><general>Springer Nature B.V</general><general>School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China</general><general>SynBio Research Platform,Collaborative Innovation Center of Chemical Science and Engineering(Tianjin),Tianjin 300072,China</general><general>Key Laboratory of System Bioengineering(Tianjin University),Ministry of Education,Tianjin 300072,China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20211001</creationdate><title>Engineering Corynebacterium glutamicum for Geraniol Production</title><author>Li, Man ; Xu, Shuo ; Lu, Wenyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-def7565b35efd47165beec63beac98dec053aa1ab67a03edaf9eb6e8be65a7203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cosmetics</topic><topic>Engineering</topic><topic>Fermentation</topic><topic>Harbor engineering</topic><topic>Humanities and Social Sciences</topic><topic>Mechanical Engineering</topic><topic>multidisciplinary</topic><topic>Optimization</topic><topic>Research Article</topic><topic>Science</topic><topic>Terpenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Man</creatorcontrib><creatorcontrib>Xu, Shuo</creatorcontrib><creatorcontrib>Lu, Wenyu</creatorcontrib><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Transactions of Tianjin University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Man</au><au>Xu, Shuo</au><au>Lu, Wenyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Engineering Corynebacterium glutamicum for Geraniol Production</atitle><jtitle>Transactions of Tianjin University</jtitle><stitle>Trans. Tianjin Univ</stitle><date>2021-10-01</date><risdate>2021</risdate><volume>27</volume><issue>5</issue><spage>377</spage><epage>384</epage><pages>377-384</pages><issn>1006-4982</issn><eissn>1995-8196</eissn><abstract>Geraniol is a monoterpenoid alcohol with various applications in food, cosmetics, and healthcare.
Corynebacterium glutamicum
is a potential platform for terpenoids production because it harbors the methylerythritol phosphate pathway. To engineer
C
.
glutamicum
to produce geraniol, two different truncated geraniol synthases (GESs) were respectively expressed, and strain LX02 expressing the truncated GESs from
Valeriana officinalis
(tVoGES) produced 0.3 mg/L of geraniol. Then, three geranyl diphosphate synthases (GPPSs) were combinatorially co-expressed with tVoGES to improve geraniol production. The amounts of produced geraniol were all higher than that produced by strain LX02. Strain LX03 co-expressing ERG20
F96W–N127W
(ERG20
WW
) and tVoGES produced the highest amount, 5.4 mg/L. Subsequently, the co-overexpression of 1-deoxy-
d
-xylulose-5-phosphate synthase (
dxs
) and isopentenyl diphosphate isomerase (
idi
) further increased the production to 12.2 mg/L in strain LX03. Lastly, the production of geraniol was increased to 15.2 mg/L via fermentation optimization. To our knowledge, this is the first report on the engineering of
C. glutamicum
to produce geraniol and thus can serve as a reference for other monoterpenoid production studies.</abstract><cop>Tianjin</cop><pub>Tianjin University</pub><doi>10.1007/s12209-020-00252-w</doi><tpages>8</tpages></addata></record> |
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| ispartof | Transactions of Tianjin University, 2021-10, Vol.27 (5), p.377-384 |
| issn | 1006-4982 1995-8196 |
| language | eng |
| recordid | cdi_wanfang_journals_tianjdxxb_e202105003 |
| source | SpringerNature Journals; Alma/SFX Local Collection |
| subjects | Cosmetics Engineering Fermentation Harbor engineering Humanities and Social Sciences Mechanical Engineering multidisciplinary Optimization Research Article Science Terpenes |
| title | Engineering Corynebacterium glutamicum for Geraniol Production |
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