Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils”
Background Primary eosinophilic gastrointestinal disorders (EGIDs) constitute chronic allergic inflammation. The number of eosinophils is one of the diagnostic criteria; more than 20 eosinophils per high-power field (HPF) in the gastrointestinal (GI) tract are considered abnormal in Japan. However,...
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| Veröffentlicht in: | World journal of pediatrics : WJP 2023-03, Vol.19 (3), p.251-260 |
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| container_title | World journal of pediatrics : WJP |
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| creator | Iwaya, Mai Kobayashi, Shota Nakayama, Yoshiko Kato, Sawako Kurasawa, Shingo Sado, Tomomitsu Iwaya, Yugo Uehara, Takeshi Ota, Hiroyoshi |
| description | Background
Primary eosinophilic gastrointestinal disorders (EGIDs) constitute chronic allergic inflammation. The number of eosinophils is one of the diagnostic criteria; more than 20 eosinophils per high-power field (HPF) in the gastrointestinal (GI) tract are considered abnormal in Japan. However, the quantity of eosinophils considered normal varies according to anatomical location and geographical region; such values have not been reported in Japanese pediatric patients, nor have the numbers of lymphocytes in the normal pediatric stomach. To establish a reference for defining diagnostic criteria for EGIDs, we evaluated the number of eosinophils in the normal Japanese pediatric GI tract.
Methods
We examined 131 biopsy cases without significant clinical history, endoscopic abnormality, or histological abnormality. Immunohistochemical analysis of CD3 and CD20 was performed.
Results
The mean eosinophil density was highest in the cecum (49.5 ± 22.4 per HPF). Counts of more than 20 eosinophils per HPF were observed in the duodenum [bulb (20.0 ± 9.6) and second portion (30.0 ± 15.8)], terminal ileum (38.3 ± 22.7), cecum (49.5 ± 22.4), ascending colon (42.3 ± 25.3), transverse colon (29.4 ± 17.0), and descending colon (32.2 ± 17.9). Counts of fewer than 10 eosinophils per HPF were observed in the stomach and rectum; a count of fewer than one eosinophil per HPF was observed in the esophagus. More than 100 CD3-positive T cells per HPF were observed in the stomach.
Conclusions
The mean numbers of eosinophils in the bowel were greater than 20 per HPF. For Japanese pediatrics, the current threshold eosinophil count should be revised. |
| doi_str_mv | 10.1007/s12519-022-00646-x |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_cross</sourceid><recordid>TN_cdi_wanfang_journals_ssekzz202303005</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>ssekzz202303005</wanfj_id><sourcerecordid>ssekzz202303005</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-8ab2e8ebdba996260ef26c2a48ed25ea44a7b9060a01f9e5e1af533c38e87ce33</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi0EoqXwAiyQd6wCviSOww5VXFXBBtbWiXM845LYkZ0Rna76IPAIvFSfBM-E24qVLZ3v_32sj5DHnD3jjLXPMxcN7yomRMWYqlV1dYeccq3aiind3i33lulKN6o7IQ9yviyQ4IrdJydS1VJxLU7Jjw-7qcdEIQx08HlJvt8tPgYaHcWYfYjz1o_5OB_307yNdr9gpj7QZYv0PcwQMCOdcfBQ0pZuoLREHwq1-AAjXRLY5cUhkRGS3R6qgQ7ofPDHp1xM9PbmG_QhpgnGcV9YmxAyDv_ucHvz_SG552DM-OjXeUY-v3716fxtdfHxzbvzlxeVretmqTT0AjX2Qw9dp4Ri6ISyAmqNg2gQ6hravmOKAeOuwwY5uEZKKzXq1qKUZ-Tp2vsVgoOwMZdxl8pfsskZv1xfCyYkk4w1hRQraVPMOaEzc_ITpL3hzBwsmdWSKZbM0ZK5KqEna2je9RMOfyK_tRRArkAuo7DB9HeB_9T-BB-cpYw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils”</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Iwaya, Mai ; Kobayashi, Shota ; Nakayama, Yoshiko ; Kato, Sawako ; Kurasawa, Shingo ; Sado, Tomomitsu ; Iwaya, Yugo ; Uehara, Takeshi ; Ota, Hiroyoshi</creator><creatorcontrib>Iwaya, Mai ; Kobayashi, Shota ; Nakayama, Yoshiko ; Kato, Sawako ; Kurasawa, Shingo ; Sado, Tomomitsu ; Iwaya, Yugo ; Uehara, Takeshi ; Ota, Hiroyoshi</creatorcontrib><description>Background
Primary eosinophilic gastrointestinal disorders (EGIDs) constitute chronic allergic inflammation. The number of eosinophils is one of the diagnostic criteria; more than 20 eosinophils per high-power field (HPF) in the gastrointestinal (GI) tract are considered abnormal in Japan. However, the quantity of eosinophils considered normal varies according to anatomical location and geographical region; such values have not been reported in Japanese pediatric patients, nor have the numbers of lymphocytes in the normal pediatric stomach. To establish a reference for defining diagnostic criteria for EGIDs, we evaluated the number of eosinophils in the normal Japanese pediatric GI tract.
Methods
We examined 131 biopsy cases without significant clinical history, endoscopic abnormality, or histological abnormality. Immunohistochemical analysis of CD3 and CD20 was performed.
Results
The mean eosinophil density was highest in the cecum (49.5 ± 22.4 per HPF). Counts of more than 20 eosinophils per HPF were observed in the duodenum [bulb (20.0 ± 9.6) and second portion (30.0 ± 15.8)], terminal ileum (38.3 ± 22.7), cecum (49.5 ± 22.4), ascending colon (42.3 ± 25.3), transverse colon (29.4 ± 17.0), and descending colon (32.2 ± 17.9). Counts of fewer than 10 eosinophils per HPF were observed in the stomach and rectum; a count of fewer than one eosinophil per HPF was observed in the esophagus. More than 100 CD3-positive T cells per HPF were observed in the stomach.
Conclusions
The mean numbers of eosinophils in the bowel were greater than 20 per HPF. For Japanese pediatrics, the current threshold eosinophil count should be revised.</description><identifier>ISSN: 1708-8569</identifier><identifier>EISSN: 1867-0687</identifier><identifier>DOI: 10.1007/s12519-022-00646-x</identifier><identifier>PMID: 36436182</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Biopsy ; Child ; Critical Care Medicine ; East Asian People ; Eosinophilia - diagnosis ; Eosinophils - pathology ; Gastrointestinal Tract - pathology ; Humans ; Imaging ; Intensive ; Lymphocytes - pathology ; Maternal and Child Health ; Medicine ; Medicine & Public Health ; Original Article ; Pediatric Surgery ; Pediatrics ; Radiology ; Surgery</subject><ispartof>World journal of pediatrics : WJP, 2023-03, Vol.19 (3), p.251-260</ispartof><rights>Children's Hospital, Zhejiang University School of Medicine 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. Children's Hospital, Zhejiang University School of Medicine.</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-8ab2e8ebdba996260ef26c2a48ed25ea44a7b9060a01f9e5e1af533c38e87ce33</citedby><cites>FETCH-LOGICAL-c445t-8ab2e8ebdba996260ef26c2a48ed25ea44a7b9060a01f9e5e1af533c38e87ce33</cites><orcidid>0000-0003-2135-2219</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/ssekzz/ssekzz.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12519-022-00646-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12519-022-00646-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36436182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iwaya, Mai</creatorcontrib><creatorcontrib>Kobayashi, Shota</creatorcontrib><creatorcontrib>Nakayama, Yoshiko</creatorcontrib><creatorcontrib>Kato, Sawako</creatorcontrib><creatorcontrib>Kurasawa, Shingo</creatorcontrib><creatorcontrib>Sado, Tomomitsu</creatorcontrib><creatorcontrib>Iwaya, Yugo</creatorcontrib><creatorcontrib>Uehara, Takeshi</creatorcontrib><creatorcontrib>Ota, Hiroyoshi</creatorcontrib><title>Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils”</title><title>World journal of pediatrics : WJP</title><addtitle>World J Pediatr</addtitle><addtitle>World J Pediatr</addtitle><description>Background
Primary eosinophilic gastrointestinal disorders (EGIDs) constitute chronic allergic inflammation. The number of eosinophils is one of the diagnostic criteria; more than 20 eosinophils per high-power field (HPF) in the gastrointestinal (GI) tract are considered abnormal in Japan. However, the quantity of eosinophils considered normal varies according to anatomical location and geographical region; such values have not been reported in Japanese pediatric patients, nor have the numbers of lymphocytes in the normal pediatric stomach. To establish a reference for defining diagnostic criteria for EGIDs, we evaluated the number of eosinophils in the normal Japanese pediatric GI tract.
Methods
We examined 131 biopsy cases without significant clinical history, endoscopic abnormality, or histological abnormality. Immunohistochemical analysis of CD3 and CD20 was performed.
Results
The mean eosinophil density was highest in the cecum (49.5 ± 22.4 per HPF). Counts of more than 20 eosinophils per HPF were observed in the duodenum [bulb (20.0 ± 9.6) and second portion (30.0 ± 15.8)], terminal ileum (38.3 ± 22.7), cecum (49.5 ± 22.4), ascending colon (42.3 ± 25.3), transverse colon (29.4 ± 17.0), and descending colon (32.2 ± 17.9). Counts of fewer than 10 eosinophils per HPF were observed in the stomach and rectum; a count of fewer than one eosinophil per HPF was observed in the esophagus. More than 100 CD3-positive T cells per HPF were observed in the stomach.
Conclusions
The mean numbers of eosinophils in the bowel were greater than 20 per HPF. For Japanese pediatrics, the current threshold eosinophil count should be revised.</description><subject>Biopsy</subject><subject>Child</subject><subject>Critical Care Medicine</subject><subject>East Asian People</subject><subject>Eosinophilia - diagnosis</subject><subject>Eosinophils - pathology</subject><subject>Gastrointestinal Tract - pathology</subject><subject>Humans</subject><subject>Imaging</subject><subject>Intensive</subject><subject>Lymphocytes - pathology</subject><subject>Maternal and Child Health</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>Radiology</subject><subject>Surgery</subject><issn>1708-8569</issn><issn>1867-0687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EoqXwAiyQd6wCviSOww5VXFXBBtbWiXM845LYkZ0Rna76IPAIvFSfBM-E24qVLZ3v_32sj5DHnD3jjLXPMxcN7yomRMWYqlV1dYeccq3aiind3i33lulKN6o7IQ9yviyQ4IrdJydS1VJxLU7Jjw-7qcdEIQx08HlJvt8tPgYaHcWYfYjz1o_5OB_307yNdr9gpj7QZYv0PcwQMCOdcfBQ0pZuoLREHwq1-AAjXRLY5cUhkRGS3R6qgQ7ofPDHp1xM9PbmG_QhpgnGcV9YmxAyDv_ucHvz_SG552DM-OjXeUY-v3716fxtdfHxzbvzlxeVretmqTT0AjX2Qw9dp4Ri6ISyAmqNg2gQ6hravmOKAeOuwwY5uEZKKzXq1qKUZ-Tp2vsVgoOwMZdxl8pfsskZv1xfCyYkk4w1hRQraVPMOaEzc_ITpL3hzBwsmdWSKZbM0ZK5KqEna2je9RMOfyK_tRRArkAuo7DB9HeB_9T-BB-cpYw</recordid><startdate>20230301</startdate><enddate>20230301</enddate><creator>Iwaya, Mai</creator><creator>Kobayashi, Shota</creator><creator>Nakayama, Yoshiko</creator><creator>Kato, Sawako</creator><creator>Kurasawa, Shingo</creator><creator>Sado, Tomomitsu</creator><creator>Iwaya, Yugo</creator><creator>Uehara, Takeshi</creator><creator>Ota, Hiroyoshi</creator><general>Springer Nature Singapore</general><general>Department of Laboratory Medicine,Shinshu University Hospital,3-1-1 Asahi,Matsumoto,Nagano,Japan%Department of Pediatrics,Shinshu University School of Medicine,Matsumoto,Japan%Division of Gastroenterology and Hepatology,Department of Medicine,Shinshu University School of Medicine,Matsumoto,Japan%Department of Laboratory Medicine,Shinshu University School of Medicine,Matsumoto,Japan%Department of Clinical Laboratory Sciences,School of Health Sciences,Shinshu University,Matsumoto,Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><orcidid>https://orcid.org/0000-0003-2135-2219</orcidid></search><sort><creationdate>20230301</creationdate><title>Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils”</title><author>Iwaya, Mai ; Kobayashi, Shota ; Nakayama, Yoshiko ; Kato, Sawako ; Kurasawa, Shingo ; Sado, Tomomitsu ; Iwaya, Yugo ; Uehara, Takeshi ; Ota, Hiroyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-8ab2e8ebdba996260ef26c2a48ed25ea44a7b9060a01f9e5e1af533c38e87ce33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Biopsy</topic><topic>Child</topic><topic>Critical Care Medicine</topic><topic>East Asian People</topic><topic>Eosinophilia - diagnosis</topic><topic>Eosinophils - pathology</topic><topic>Gastrointestinal Tract - pathology</topic><topic>Humans</topic><topic>Imaging</topic><topic>Intensive</topic><topic>Lymphocytes - pathology</topic><topic>Maternal and Child Health</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>Radiology</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iwaya, Mai</creatorcontrib><creatorcontrib>Kobayashi, Shota</creatorcontrib><creatorcontrib>Nakayama, Yoshiko</creatorcontrib><creatorcontrib>Kato, Sawako</creatorcontrib><creatorcontrib>Kurasawa, Shingo</creatorcontrib><creatorcontrib>Sado, Tomomitsu</creatorcontrib><creatorcontrib>Iwaya, Yugo</creatorcontrib><creatorcontrib>Uehara, Takeshi</creatorcontrib><creatorcontrib>Ota, Hiroyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>World journal of pediatrics : WJP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iwaya, Mai</au><au>Kobayashi, Shota</au><au>Nakayama, Yoshiko</au><au>Kato, Sawako</au><au>Kurasawa, Shingo</au><au>Sado, Tomomitsu</au><au>Iwaya, Yugo</au><au>Uehara, Takeshi</au><au>Ota, Hiroyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils”</atitle><jtitle>World journal of pediatrics : WJP</jtitle><stitle>World J Pediatr</stitle><addtitle>World J Pediatr</addtitle><date>2023-03-01</date><risdate>2023</risdate><volume>19</volume><issue>3</issue><spage>251</spage><epage>260</epage><pages>251-260</pages><issn>1708-8569</issn><eissn>1867-0687</eissn><abstract>Background
Primary eosinophilic gastrointestinal disorders (EGIDs) constitute chronic allergic inflammation. The number of eosinophils is one of the diagnostic criteria; more than 20 eosinophils per high-power field (HPF) in the gastrointestinal (GI) tract are considered abnormal in Japan. However, the quantity of eosinophils considered normal varies according to anatomical location and geographical region; such values have not been reported in Japanese pediatric patients, nor have the numbers of lymphocytes in the normal pediatric stomach. To establish a reference for defining diagnostic criteria for EGIDs, we evaluated the number of eosinophils in the normal Japanese pediatric GI tract.
Methods
We examined 131 biopsy cases without significant clinical history, endoscopic abnormality, or histological abnormality. Immunohistochemical analysis of CD3 and CD20 was performed.
Results
The mean eosinophil density was highest in the cecum (49.5 ± 22.4 per HPF). Counts of more than 20 eosinophils per HPF were observed in the duodenum [bulb (20.0 ± 9.6) and second portion (30.0 ± 15.8)], terminal ileum (38.3 ± 22.7), cecum (49.5 ± 22.4), ascending colon (42.3 ± 25.3), transverse colon (29.4 ± 17.0), and descending colon (32.2 ± 17.9). Counts of fewer than 10 eosinophils per HPF were observed in the stomach and rectum; a count of fewer than one eosinophil per HPF was observed in the esophagus. More than 100 CD3-positive T cells per HPF were observed in the stomach.
Conclusions
The mean numbers of eosinophils in the bowel were greater than 20 per HPF. For Japanese pediatrics, the current threshold eosinophil count should be revised.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>36436182</pmid><doi>10.1007/s12519-022-00646-x</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2135-2219</orcidid></addata></record> |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Biopsy Child Critical Care Medicine East Asian People Eosinophilia - diagnosis Eosinophils - pathology Gastrointestinal Tract - pathology Humans Imaging Intensive Lymphocytes - pathology Maternal and Child Health Medicine Medicine & Public Health Original Article Pediatric Surgery Pediatrics Radiology Surgery |
| title | Number and distribution of eosinophils and lymphocytes in the Japanese pediatric gastrointestinal tract: in search of a definition for “abnormally increased eosinophils” |
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