基于金纳米花的双信号适配体传感器检测红酒中真菌毒素

基于金纳米花的双信号适配体传感器检测红酒中真菌毒素

TS207.3; 构建基于金纳米花(gold nanoflower,AuFL)和适配体的双信号荧光适配体传感器用于同时检测赭曲霉毒素A(ochratoxin A,OTA)和玉米赤霉烯酮(zearalenone,ZEN).以AuFL为载体,将含OTA和ZEN适配体的单链DNA共价交联在AuFL表面,进一步将其与两种荧光染料(FAM和Cy3)标记的互补DNA杂交,制备DNA功能化纳米探针.荧光染料和AuFL之间发生荧光共振能量转移(fluorescence resonance energy transfer,FRET)导致纳米探针自身无荧光.加入OTA和ZEN后,FAM和Cy3从探针表面脱落会扰乱...

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Veröffentlicht in:食品科学 2024-01, Vol.45 (2), p.308-314
Hauptverfasser: 祁兴普, 董骐玮, 朱麟菲, 邹婷婷, 郑义, 张婧怡, 李倩
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container_end_page 314
container_issue 2
container_start_page 308
container_title 食品科学
container_volume 45
creator 祁兴普
董骐玮
朱麟菲
邹婷婷
郑义
张婧怡
李倩
description TS207.3; 构建基于金纳米花(gold nanoflower,AuFL)和适配体的双信号荧光适配体传感器用于同时检测赭曲霉毒素A(ochratoxin A,OTA)和玉米赤霉烯酮(zearalenone,ZEN).以AuFL为载体,将含OTA和ZEN适配体的单链DNA共价交联在AuFL表面,进一步将其与两种荧光染料(FAM和Cy3)标记的互补DNA杂交,制备DNA功能化纳米探针.荧光染料和AuFL之间发生荧光共振能量转移(fluorescence resonance energy transfer,FRET)导致纳米探针自身无荧光.加入OTA和ZEN后,FAM和Cy3从探针表面脱落会扰乱FRET过程,导致两者荧光的恢复.优化条件下,该双信号荧光适配体传感器对OTA检测的线性范围为0.05～500 ng/mL,检出限为0.017 ng/mL.对ZEN检测的线性范围为0.1～500 ng/mL,检出限为0.033 ng/mL.该双信号荧光适配体传感器具有良好的选择性和重现性,并能够成功应用于红酒实际样中OTA和ZEN的检测.
doi_str_mv 10.7506/spkx1002-6630-20230418-179
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title 基于金纳米花的双信号适配体传感器检测红酒中真菌毒素
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