利拉鲁肽对高糖诱导内皮细胞miRNA-146b-3p、炎症因子及环氧化酶-2表达的影响
R587.1; 目的·探讨利拉鲁肽对高糖诱导人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs) miR-146b-3p及白细胞介素-6 (interleulin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α、环氧化酶-2(cyclooxygenase-2,COX-2)表达的影响.方法·高糖组和对照组HUVECs分别以高浓度葡萄糖(25 mmol/L)及正常浓度葡萄糖(7mmol/L)处理24 h,再以利拉鲁肽分别干预上述2组细胞.定量聚合酶链反应(quantitative polymera...
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| Veröffentlicht in: | 上海交通大学学报（医学版） 2018-12, Vol.38 (12), p.1420-1424 |
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| container_title | 上海交通大学学报（医学版） |
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| creator | 苏征佳 潘晓茜 曹久妹 吴方 |
| description | R587.1; 目的·探讨利拉鲁肽对高糖诱导人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs) miR-146b-3p及白细胞介素-6 (interleulin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α、环氧化酶-2(cyclooxygenase-2,COX-2)表达的影响.方法·高糖组和对照组HUVECs分别以高浓度葡萄糖(25 mmol/L)及正常浓度葡萄糖(7mmol/L)处理24 h,再以利拉鲁肽分别干预上述2组细胞.定量聚合酶链反应(quantitative polymerase chain reaction,qPCR)检测各组miR-146b-3p、IL-6、TNF-α、COX-2的表达变化.抑制miR-146b-3p后观察炎症因子及COX-2的表达变化.结果·在高糖诱导的HUVECs中,miR-146b-3p的表达下降,IL-6、TNF-α及COX-2的表达均升高.抑制miR-146b-3p表达会显著升高高糖诱导的HUVECs中IL-6、TNF-α及COX-2的表达.利拉鲁肽可显著升高高糖诱导的HUVECs中miR-146b-3p的表达,降低IL-6、TNF-α及COX-2的表达.结论·在高糖环境下,利拉鲁肽可能通过升高miR-146b-3p的表达而降低IL-6、TNF-α及COX-2的表达,从而改善炎症和血管内皮功能. |
| doi_str_mv | 10.3969/j.issn.1674-8115.2018.12.004 |
| format | Article |
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| title | 利拉鲁肽对高糖诱导内皮细胞miRNA-146b-3p、炎症因子及环氧化酶-2表达的影响 |
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