重金属超富集植物东南景天毛状根的诱导
为建立重金属超富集植物东南景天(Sedumalfredii)的毛状根诱导体系,采用发根农杆菌(Agrobacteriumrhizogenes)A4侵染叶片,研究了预培养时间、侵染时间和共培养时间对毛状根诱导率的影响。结果表明,东南景天叶片外植体的预培养时间为48h、农杆菌侵染时间为6min、共培养时间为48h是适宜的毛状根诱导时间,毛状根的诱导率可达85%。PCR检测表明诱导的毛状根中存在rolB基因片段。这是东南景天首次建立用发根农杆菌诱导毛状根体系。...
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| Veröffentlicht in: | 热带亚热带植物学报 2017, Vol.25 (2), p.136-140 |
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| container_title | 热带亚热带植物学报 |
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| creator | 陈友明 刘静轶 张腾 卢倩云 晏琼 |
| description | 为建立重金属超富集植物东南景天(Sedumalfredii)的毛状根诱导体系,采用发根农杆菌(Agrobacteriumrhizogenes)A4侵染叶片,研究了预培养时间、侵染时间和共培养时间对毛状根诱导率的影响。结果表明,东南景天叶片外植体的预培养时间为48h、农杆菌侵染时间为6min、共培养时间为48h是适宜的毛状根诱导时间,毛状根的诱导率可达85%。PCR检测表明诱导的毛状根中存在rolB基因片段。这是东南景天首次建立用发根农杆菌诱导毛状根体系。 |
| doi_str_mv | 10.11926/jtsb.3644 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1005-3395 |
| ispartof | 热带亚热带植物学报, 2017, Vol.25 (2), p.136-140 |
| issn | 1005-3395 |
| language | chi |
| recordid | cdi_wanfang_journals_rdyrdzwxb201702005 |
| source | Alma/SFX Local Collection |
| subjects | 重金属超富集植物 东南景天 毛状根 遗传转化 |
| title | 重金属超富集植物东南景天毛状根的诱导 |
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