辣木组织培养与四倍体植株诱导
建立了印度辣木(Moringa oleifera)和非洲辣木(Moringa stenopetala)离体再生体系,并利用秋水仙碱进行了四倍体的诱导。结果表明:离体培养时以茎段为外植体可快速获得无菌苗,愈伤组织诱导与不定芽分化时,印度辣木以MS+BA0.6mgL^-1+NAA0.1mg L^-1的固体培养基为最佳,非洲辣木以MS+BA0.5mgL^-1+NAA0.2mgL^-1的固体培养基为最佳,生根培养基均以MS+IBA0.2mg L^-1最适。用不同浓度秋水仙碱对染色体进行加倍诱变,以0.2%秋水仙碱处理5d,加倍效果最好,印度辣木达到40.0%,非洲辣木为36.7%。获得了印度辣木和非洲...
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creator | 向素琼 梁国鲁 郭启高 李晓林 何桥 |
description | 建立了印度辣木(Moringa oleifera)和非洲辣木(Moringa stenopetala)离体再生体系,并利用秋水仙碱进行了四倍体的诱导。结果表明:离体培养时以茎段为外植体可快速获得无菌苗,愈伤组织诱导与不定芽分化时,印度辣木以MS+BA0.6mgL^-1+NAA0.1mg L^-1的固体培养基为最佳,非洲辣木以MS+BA0.5mgL^-1+NAA0.2mgL^-1的固体培养基为最佳,生根培养基均以MS+IBA0.2mg L^-1最适。用不同浓度秋水仙碱对染色体进行加倍诱变,以0.2%秋水仙碱处理5d,加倍效果最好,印度辣木达到40.0%,非洲辣木为36.7%。获得了印度辣木和非洲辣木四倍体新种质,四倍体植株初步表现枝粗、叶片变大变厚且叶色更绿等特征。 |
doi_str_mv | 10.3969/j.issn.1005-3395.2007.02.008 |
format | Article |
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issn | 1005-3395 |
language | chi |
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subjects | 印度辣木 四倍体 秋水仙碱 组织培养 非洲辣木 |
title | 辣木组织培养与四倍体植株诱导 |
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