Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green
Malachite green （MG）, a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabo- lized into reduced leucomalachite green forms （LMG） that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a c...
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| Veröffentlicht in: | Journal of Ocean University of China 2015-04, Vol.14 (2), p.340-344 |
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| creator | Jiang, Yousheng Chen, Li Hu, Kun Yu, Wenjuan Yang, Xianle Lu, Liqun |
| description | Malachite green （MG）, a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabo- lized into reduced leucomalachite green forms （LMG） that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay （ELISA） to detect MG and LMG specifically. The monoclonal antibody （mAb） to LMG was generated using a hybridoma technique. The obtained mAb showed good cross-reactivity （CR） to malachite green （MG）, but not to crystal violet （CV） and Brilliant Green （BG）. The mAb was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37ngmL-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coeffi- cients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG. |
| doi_str_mv | 10.1007/s11802-015-2407-5 |
| format | Article |
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It is metabo- lized into reduced leucomalachite green forms （LMG） that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay （ELISA） to detect MG and LMG specifically. The monoclonal antibody （mAb） to LMG was generated using a hybridoma technique. The obtained mAb showed good cross-reactivity （CR） to malachite green （MG）, but not to crystal violet （CV） and Brilliant Green （BG）. The mAb was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37ngmL-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coeffi- cients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG.</description><identifier>ISSN: 1672-5182</identifier><identifier>EISSN: 1993-5021</identifier><identifier>EISSN: 1672-5174</identifier><identifier>DOI: 10.1007/s11802-015-2407-5</identifier><language>eng</language><publisher>Heidelberg: Science Press</publisher><subject>Disease ; Dyes ; Earth and Environmental Science ; Earth Sciences ; ELISA ; Fish ; Fish diseases ; Freshwater ; Fungi ; Marine ; Metabolism ; Meteorology ; Muscles ; Oceanography ; 单克隆抗体 ; 开发 ; 杂交瘤技术 ; 特异性检测 ; 酶联免疫吸附测定 ; 酶联免疫吸附试验 ; 隐性孔雀石绿</subject><ispartof>Journal of Ocean University of China, 2015-04, Vol.14 (2), p.340-344</ispartof><rights>Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg 2015</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-34d188d868522cf0915f5f320e4a9344b697f7efdae0f3307f3dd53adfb4e90d3</citedby><cites>FETCH-LOGICAL-c482t-34d188d868522cf0915f5f320e4a9344b697f7efdae0f3307f3dd53adfb4e90d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/87473A/87473A.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11802-015-2407-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11802-015-2407-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Jiang, Yousheng</creatorcontrib><creatorcontrib>Chen, Li</creatorcontrib><creatorcontrib>Hu, Kun</creatorcontrib><creatorcontrib>Yu, Wenjuan</creatorcontrib><creatorcontrib>Yang, Xianle</creatorcontrib><creatorcontrib>Lu, Liqun</creatorcontrib><title>Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green</title><title>Journal of Ocean University of China</title><addtitle>J. Ocean Univ. China</addtitle><addtitle>Journal of Ocean University of China</addtitle><description>Malachite green （MG）, a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabo- lized into reduced leucomalachite green forms （LMG） that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay （ELISA） to detect MG and LMG specifically. The monoclonal antibody （mAb） to LMG was generated using a hybridoma technique. The obtained mAb showed good cross-reactivity （CR） to malachite green （MG）, but not to crystal violet （CV） and Brilliant Green （BG）. The mAb was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37ngmL-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coeffi- cients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG.</description><subject>Disease</subject><subject>Dyes</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>ELISA</subject><subject>Fish</subject><subject>Fish diseases</subject><subject>Freshwater</subject><subject>Fungi</subject><subject>Marine</subject><subject>Metabolism</subject><subject>Meteorology</subject><subject>Muscles</subject><subject>Oceanography</subject><subject>单克隆抗体</subject><subject>开发</subject><subject>杂交瘤技术</subject><subject>特异性检测</subject><subject>酶联免疫吸附测定</subject><subject>酶联免疫吸附试验</subject><subject>隐性孔雀石绿</subject><issn>1672-5182</issn><issn>1993-5021</issn><issn>1672-5174</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kUtr3DAUhU1pIY_mB2Qn2k0gONXDkuxlyKuBKVkkWQuNdDV28EgzkqZN_n1kHEKbRdFC4vKdcy46VXVM8BnBWP5IhLSY1pjwmjZY1vxTtU-6jtUcU_K5vIWkNSct3asOUnrCmDMu5H61uoTfMIbNGnxGwSGNrnXK6Gpxe3-OXIgo94DuN2AGNxh0CRlMHoKf0GXIPVrAzoS1HrXphwzoJgJ4pL1Fv_6dfa2-OD0mOHq7D6vH66uHi5_14u7m9uJ8UZumpblmjSVta1vRckqNwx3hjjtGMTS6Y02zFJ10EpzVgB1jWDpmLWfaumUDHbbssDqdff9o77Rfqaewi74kqq3tX-zz81IBLb-EaTmFPpnpTQzbHaSs1kMyMI7aQ9glRYRoKJGUs4J-_4C-OxPBBRVE8smQzJSJIaUITm3isNbxRRGspqLUXJQqK6ipKMWLhs6aVFi_gviX839E396C-uBX26J7Tyo7M86n8l8BLqCfRA</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Jiang, Yousheng</creator><creator>Chen, Li</creator><creator>Hu, Kun</creator><creator>Yu, Wenjuan</creator><creator>Yang, Xianle</creator><creator>Lu, Liqun</creator><general>Science Press</general><general>Springer Nature B.V</general><general>College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, P.R.China</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7TN</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H96</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>M2P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>H97</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>20150401</creationdate><title>Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green</title><author>Jiang, Yousheng ; 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Ocean Univ. China</stitle><addtitle>Journal of Ocean University of China</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>14</volume><issue>2</issue><spage>340</spage><epage>344</epage><pages>340-344</pages><issn>1672-5182</issn><eissn>1993-5021</eissn><eissn>1672-5174</eissn><abstract>Malachite green （MG）, a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabo- lized into reduced leucomalachite green forms （LMG） that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay （ELISA） to detect MG and LMG specifically. The monoclonal antibody （mAb） to LMG was generated using a hybridoma technique. The obtained mAb showed good cross-reactivity （CR） to malachite green （MG）, but not to crystal violet （CV） and Brilliant Green （BG）. The mAb was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37ngmL-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coeffi- cients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG.</abstract><cop>Heidelberg</cop><pub>Science Press</pub><doi>10.1007/s11802-015-2407-5</doi><tpages>5</tpages></addata></record> |
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| subjects | Disease Dyes Earth and Environmental Science Earth Sciences ELISA Fish Fish diseases Freshwater Fungi Marine Metabolism Meteorology Muscles Oceanography 单克隆抗体 开发 杂交瘤技术 特异性检测 酶联免疫吸附测定 酶联免疫吸附试验 隐性孔雀石绿 |
| title | Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green |
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