Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)
Cytochrome P450 aromatase (P450arom) plays a pivotal role in ovary development. In this study, we used semi-quantitative reverse transcription PCR (RT-PCR) to analyze spatiotemporal expressions of two P450arom genes ( CYP19A and CYP19B ) and their responses to temperature stress in female yellow cat...
Gespeichert in:
| Veröffentlicht in: | Journal of Ocean University of China 2011-12, Vol.10 (4), p.409-416 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 416 |
|---|---|
| container_issue | 4 |
| container_start_page | 409 |
| container_title | Journal of Ocean University of China |
| container_volume | 10 |
| creator | Liu, Miao Wen, Haishen He, Feng Li, Jifang Shi, Dan Hu, Jian Zhang, Yuanqing Ma, Ruiqin Mu, Weijie Qi, Baoxia |
| description | Cytochrome P450 aromatase (P450arom) plays a pivotal role in ovary development. In this study, we used semi-quantitative reverse transcription PCR (RT-PCR) to analyze spatiotemporal expressions of two P450arom genes (
CYP19A
and
CYP19B
) and their responses to temperature stress in female yellow catfish (
Pelteobagrus fulvidraco
). Tissue distribution pattern of
CYP19
showed that
CYP19B
was abundantly expressed in fish brain and ovary (brain > ovary), but weakly in intestines, whereas
CYP19A
was exclusively expressed in ovary. Semi-quantitative RT-PCR analyses showed high transcript abundance of both
CYP19A
and
CYP19B
in the ovarian reproductive cycle, corresponding with serum estradiol-17
β
(E
2
) levels. Increases in aromatases, serum E
2
and testosterone (T) levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages II and V during the ovarian cycle, whereas associated decreases in stage III suggested vitellogenesis inhibition by heat stress. Gene expression of
CYP19
was closely related to levels of serum E
2
. Results demonstrated
CYP19
played a crucial role in the reproductive cycle of female yellow catfish. Different temperature stress affected
CYP19
gene expression in the fish ovarian reproductive cycle. Associated P450arom genes could be useful for studying physiological aspects of yellow catfish. |
| doi_str_mv | 10.1007/s11802-011-1835-0 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_proqu</sourceid><recordid>TN_cdi_wanfang_journals_qdhydxxb_e201104016</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><wanfj_id>qdhydxxb_e201104016</wanfj_id><sourcerecordid>qdhydxxb_e201104016</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2986-9c37e328bcdea060c15470494f43afeac88fc6c0e5cbf893293ef0bdeb602c413</originalsourceid><addsrcrecordid>eNp1kc1u1DAUhSMEEqXwAOyu2ECFAtfOn71EVYFKlegC1pbjXM-kSuzUdqYz78RD4mFQkZBY3bv4zrk_pyheM_zAELuPkTGBvETGSiaqpsQnxRmTsiob5Oxp7tuOlw0T_HnxIsY7xKZq2u6s-Hm1XwLFOHoHi06JgovgLZhD8mYb_ExwWzcIOrc66UiwIUcRhjWMbgN-p8MBBtrR5JeZXALtBkhbGgNk28W7mOHkIdG8UNBpDQQxHSfC6MDSrCeCA02TfwCjkx3jFt7d0pTI93oT1gh2nXbjELTxFy-LZ1ZPkV79qefFj89X3y-_ljffvlxffropDZeiLaWpOqq46M1AGls0rKk7rGVt60pb0kYIa1qD1JjeCllxWZHFfqC-RW5qVp0X70--D9pZ7Tbqzq_B5Ynqftgehv2-V8Tzq7FG1mb67Ylegr9fKSY1j9Hkk7Qjv0YleSskr3-Tb_4hH40lct5lN5EhdoJM8DEGsmoJ45zfrBiqY9bqlLXKC6hj1gqzhp80cTnGQuGv8f9FvwCTB7Bm</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>902270168</pqid></control><display><type>article</type><title>Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)</title><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Liu, Miao ; Wen, Haishen ; He, Feng ; Li, Jifang ; Shi, Dan ; Hu, Jian ; Zhang, Yuanqing ; Ma, Ruiqin ; Mu, Weijie ; Qi, Baoxia</creator><creatorcontrib>Liu, Miao ; Wen, Haishen ; He, Feng ; Li, Jifang ; Shi, Dan ; Hu, Jian ; Zhang, Yuanqing ; Ma, Ruiqin ; Mu, Weijie ; Qi, Baoxia</creatorcontrib><description>Cytochrome P450 aromatase (P450arom) plays a pivotal role in ovary development. In this study, we used semi-quantitative reverse transcription PCR (RT-PCR) to analyze spatiotemporal expressions of two P450arom genes (
CYP19A
and
CYP19B
) and their responses to temperature stress in female yellow catfish (
Pelteobagrus fulvidraco
). Tissue distribution pattern of
CYP19
showed that
CYP19B
was abundantly expressed in fish brain and ovary (brain > ovary), but weakly in intestines, whereas
CYP19A
was exclusively expressed in ovary. Semi-quantitative RT-PCR analyses showed high transcript abundance of both
CYP19A
and
CYP19B
in the ovarian reproductive cycle, corresponding with serum estradiol-17
β
(E
2
) levels. Increases in aromatases, serum E
2
and testosterone (T) levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages II and V during the ovarian cycle, whereas associated decreases in stage III suggested vitellogenesis inhibition by heat stress. Gene expression of
CYP19
was closely related to levels of serum E
2
. Results demonstrated
CYP19
played a crucial role in the reproductive cycle of female yellow catfish. Different temperature stress affected
CYP19
gene expression in the fish ovarian reproductive cycle. Associated P450arom genes could be useful for studying physiological aspects of yellow catfish.</description><identifier>ISSN: 1672-5182</identifier><identifier>EISSN: 1993-5021</identifier><identifier>EISSN: 1672-5174</identifier><identifier>DOI: 10.1007/s11802-011-1835-0</identifier><language>eng</language><publisher>Heidelberg: SP Science Press</publisher><subject>Animal reproduction ; Catfish ; Cytochrome ; Distribution patterns ; Earth and Environmental Science ; Earth Sciences ; Females ; Gene expression ; Heat tolerance ; High temperature ; Marine ; Meteorology ; Oceanography ; Pelteobagrus fulvidraco ; Polymerase chain reaction ; Reproductive cycle ; Stress response</subject><ispartof>Journal of Ocean University of China, 2011-12, Vol.10 (4), p.409-416</ispartof><rights>Science Press, Ocean University of China and Springer-Verlag Berlin Heidelberg 2011</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2986-9c37e328bcdea060c15470494f43afeac88fc6c0e5cbf893293ef0bdeb602c413</citedby><cites>FETCH-LOGICAL-c2986-9c37e328bcdea060c15470494f43afeac88fc6c0e5cbf893293ef0bdeb602c413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/qdhydxxb-e/qdhydxxb-e.jpg</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11802-011-1835-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11802-011-1835-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Liu, Miao</creatorcontrib><creatorcontrib>Wen, Haishen</creatorcontrib><creatorcontrib>He, Feng</creatorcontrib><creatorcontrib>Li, Jifang</creatorcontrib><creatorcontrib>Shi, Dan</creatorcontrib><creatorcontrib>Hu, Jian</creatorcontrib><creatorcontrib>Zhang, Yuanqing</creatorcontrib><creatorcontrib>Ma, Ruiqin</creatorcontrib><creatorcontrib>Mu, Weijie</creatorcontrib><creatorcontrib>Qi, Baoxia</creatorcontrib><title>Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)</title><title>Journal of Ocean University of China</title><addtitle>J. Ocean Univ. China</addtitle><description>Cytochrome P450 aromatase (P450arom) plays a pivotal role in ovary development. In this study, we used semi-quantitative reverse transcription PCR (RT-PCR) to analyze spatiotemporal expressions of two P450arom genes (
CYP19A
and
CYP19B
) and their responses to temperature stress in female yellow catfish (
Pelteobagrus fulvidraco
). Tissue distribution pattern of
CYP19
showed that
CYP19B
was abundantly expressed in fish brain and ovary (brain > ovary), but weakly in intestines, whereas
CYP19A
was exclusively expressed in ovary. Semi-quantitative RT-PCR analyses showed high transcript abundance of both
CYP19A
and
CYP19B
in the ovarian reproductive cycle, corresponding with serum estradiol-17
β
(E
2
) levels. Increases in aromatases, serum E
2
and testosterone (T) levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages II and V during the ovarian cycle, whereas associated decreases in stage III suggested vitellogenesis inhibition by heat stress. Gene expression of
CYP19
was closely related to levels of serum E
2
. Results demonstrated
CYP19
played a crucial role in the reproductive cycle of female yellow catfish. Different temperature stress affected
CYP19
gene expression in the fish ovarian reproductive cycle. Associated P450arom genes could be useful for studying physiological aspects of yellow catfish.</description><subject>Animal reproduction</subject><subject>Catfish</subject><subject>Cytochrome</subject><subject>Distribution patterns</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Females</subject><subject>Gene expression</subject><subject>Heat tolerance</subject><subject>High temperature</subject><subject>Marine</subject><subject>Meteorology</subject><subject>Oceanography</subject><subject>Pelteobagrus fulvidraco</subject><subject>Polymerase chain reaction</subject><subject>Reproductive cycle</subject><subject>Stress response</subject><issn>1672-5182</issn><issn>1993-5021</issn><issn>1672-5174</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kc1u1DAUhSMEEqXwAOyu2ECFAtfOn71EVYFKlegC1pbjXM-kSuzUdqYz78RD4mFQkZBY3bv4zrk_pyheM_zAELuPkTGBvETGSiaqpsQnxRmTsiob5Oxp7tuOlw0T_HnxIsY7xKZq2u6s-Hm1XwLFOHoHi06JgovgLZhD8mYb_ExwWzcIOrc66UiwIUcRhjWMbgN-p8MBBtrR5JeZXALtBkhbGgNk28W7mOHkIdG8UNBpDQQxHSfC6MDSrCeCA02TfwCjkx3jFt7d0pTI93oT1gh2nXbjELTxFy-LZ1ZPkV79qefFj89X3y-_ljffvlxffropDZeiLaWpOqq46M1AGls0rKk7rGVt60pb0kYIa1qD1JjeCllxWZHFfqC-RW5qVp0X70--D9pZ7Tbqzq_B5Ynqftgehv2-V8Tzq7FG1mb67Ylegr9fKSY1j9Hkk7Qjv0YleSskr3-Tb_4hH40lct5lN5EhdoJM8DEGsmoJ45zfrBiqY9bqlLXKC6hj1gqzhp80cTnGQuGv8f9FvwCTB7Bm</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Liu, Miao</creator><creator>Wen, Haishen</creator><creator>He, Feng</creator><creator>Li, Jifang</creator><creator>Shi, Dan</creator><creator>Hu, Jian</creator><creator>Zhang, Yuanqing</creator><creator>Ma, Ruiqin</creator><creator>Mu, Weijie</creator><creator>Qi, Baoxia</creator><general>SP Science Press</general><general>Springer Nature B.V</general><general>Fisheries College, Ocean University of China, Qingdao 266003, P. R. China%College of Animnal Science and Technology, Inner Mongolia Nationality University, Tongliao 028000, P. R. China</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7TN</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H95</scope><scope>H96</scope><scope>HCIFZ</scope><scope>L.G</scope><scope>M2P</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope></search><sort><creationdate>201112</creationdate><title>Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)</title><author>Liu, Miao ; Wen, Haishen ; He, Feng ; Li, Jifang ; Shi, Dan ; Hu, Jian ; Zhang, Yuanqing ; Ma, Ruiqin ; Mu, Weijie ; Qi, Baoxia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2986-9c37e328bcdea060c15470494f43afeac88fc6c0e5cbf893293ef0bdeb602c413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal reproduction</topic><topic>Catfish</topic><topic>Cytochrome</topic><topic>Distribution patterns</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Females</topic><topic>Gene expression</topic><topic>Heat tolerance</topic><topic>High temperature</topic><topic>Marine</topic><topic>Meteorology</topic><topic>Oceanography</topic><topic>Pelteobagrus fulvidraco</topic><topic>Polymerase chain reaction</topic><topic>Reproductive cycle</topic><topic>Stress response</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Miao</creatorcontrib><creatorcontrib>Wen, Haishen</creatorcontrib><creatorcontrib>He, Feng</creatorcontrib><creatorcontrib>Li, Jifang</creatorcontrib><creatorcontrib>Shi, Dan</creatorcontrib><creatorcontrib>Hu, Jian</creatorcontrib><creatorcontrib>Zhang, Yuanqing</creatorcontrib><creatorcontrib>Ma, Ruiqin</creatorcontrib><creatorcontrib>Mu, Weijie</creatorcontrib><creatorcontrib>Qi, Baoxia</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><jtitle>Journal of Ocean University of China</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Miao</au><au>Wen, Haishen</au><au>He, Feng</au><au>Li, Jifang</au><au>Shi, Dan</au><au>Hu, Jian</au><au>Zhang, Yuanqing</au><au>Ma, Ruiqin</au><au>Mu, Weijie</au><au>Qi, Baoxia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco)</atitle><jtitle>Journal of Ocean University of China</jtitle><stitle>J. Ocean Univ. China</stitle><date>2011-12</date><risdate>2011</risdate><volume>10</volume><issue>4</issue><spage>409</spage><epage>416</epage><pages>409-416</pages><issn>1672-5182</issn><eissn>1993-5021</eissn><eissn>1672-5174</eissn><abstract>Cytochrome P450 aromatase (P450arom) plays a pivotal role in ovary development. In this study, we used semi-quantitative reverse transcription PCR (RT-PCR) to analyze spatiotemporal expressions of two P450arom genes (
CYP19A
and
CYP19B
) and their responses to temperature stress in female yellow catfish (
Pelteobagrus fulvidraco
). Tissue distribution pattern of
CYP19
showed that
CYP19B
was abundantly expressed in fish brain and ovary (brain > ovary), but weakly in intestines, whereas
CYP19A
was exclusively expressed in ovary. Semi-quantitative RT-PCR analyses showed high transcript abundance of both
CYP19A
and
CYP19B
in the ovarian reproductive cycle, corresponding with serum estradiol-17
β
(E
2
) levels. Increases in aromatases, serum E
2
and testosterone (T) levels in fish exposed to higher temperature indicated stimulation of ovarian maturation and recrudescence by heat stress in stages II and V during the ovarian cycle, whereas associated decreases in stage III suggested vitellogenesis inhibition by heat stress. Gene expression of
CYP19
was closely related to levels of serum E
2
. Results demonstrated
CYP19
played a crucial role in the reproductive cycle of female yellow catfish. Different temperature stress affected
CYP19
gene expression in the fish ovarian reproductive cycle. Associated P450arom genes could be useful for studying physiological aspects of yellow catfish.</abstract><cop>Heidelberg</cop><pub>SP Science Press</pub><doi>10.1007/s11802-011-1835-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-5182 |
| ispartof | Journal of Ocean University of China, 2011-12, Vol.10 (4), p.409-416 |
| issn | 1672-5182 1993-5021 1672-5174 |
| language | eng |
| recordid | cdi_wanfang_journals_qdhydxxb_e201104016 |
| source | SpringerLink Journals; Alma/SFX Local Collection |
| subjects | Animal reproduction Catfish Cytochrome Distribution patterns Earth and Environmental Science Earth Sciences Females Gene expression Heat tolerance High temperature Marine Meteorology Oceanography Pelteobagrus fulvidraco Polymerase chain reaction Reproductive cycle Stress response |
| title | Expression patterns of cytochrome P450 aromatase genes during ovary development and their responses to temperature stress in female yellow catfish (Pelteobagrus fulvidraco) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T06%3A33%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wanfang_jour_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20patterns%20of%20cytochrome%20P450%20aromatase%20genes%20during%20ovary%20development%20and%20their%20responses%20to%20temperature%20stress%20in%20female%20yellow%20catfish%20(Pelteobagrus%20fulvidraco)&rft.jtitle=Journal%20of%20Ocean%20University%20of%20China&rft.au=Liu,%20Miao&rft.date=2011-12&rft.volume=10&rft.issue=4&rft.spage=409&rft.epage=416&rft.pages=409-416&rft.issn=1672-5182&rft.eissn=1993-5021&rft_id=info:doi/10.1007/s11802-011-1835-0&rft_dat=%3Cwanfang_jour_proqu%3Eqdhydxxb_e201104016%3C/wanfang_jour_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=902270168&rft_id=info:pmid/&rft_wanfj_id=qdhydxxb_e201104016&rfr_iscdi=true |